Angptl3, CPTAC-4016 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Angptl3	N/A

Sequence Length (AA)	Molecular Weight (Da)
455	52543

Protein Name
Angiopoietin-related protein 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MHTIKLFLFV	VPLVIASRVD	PDLSSFDSAP	SEPKSRFAML	DDVKILANGL
60	70	80	90	100
LQLGHGLKDF	VHKTKGQIND	IFQKLNIFDQ	SFYDLSLRTN	EIKEEEKELR
110	120	130	140	150
RTTSTLQVKN	EEVKNMSVEL	NSKLESLLEE	KTALQHKVRA	LEEQLTNLIL
160	170	180	190	200
SPAGAQEHPE	VTSLKSFVEQ	QDNSIRELLQ	SVEEQYKQLS	QQHMQIKEIE
210	220	230	240	250
KQLRKTGIQE	PSENSLSSKS	RAPRTTPPLQ	LNETENTEQD	DLPADCSAVY
260	270	280	290	300
NRGEHTSGVY	TIKPRNSQGF	NVYCDTQSGS	PWTLIQHRKD	GSQDFNETWE
310	320	330	340	350
NYEKGFGRLD	GEFWLGLEKI	YAIVQQSNYI	LRLELQDWKD	SKHYVEYSFH
360	370	380	390	400
LGSHETNYTL	HVAEIAGNIP	GALPEHTDLM	FSTWNHRAKG	QLYCPESYSG
410	420	430	440	450
GWWWNDICGE	NNLNGKYNKP	RTKSRPERRR	GIYWRPQSRK	LYAIKSSKMM
455
LQPTT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node

to view detailed assay information below

All other points link out to UniProt

Assay Details for non-CPTAC-4016 Collapse assay details

Data source: Panorama

Official Gene Symbol: Angptl3
Peptide Sequence: GQINDIFQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 74

CPTAC ID: non-CPTAC-4016
Peptide Molecular Mass: 1,061.5506
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	5.1	3.6	1.9	9.5	4.6	2.1	10.8	5.8	2.8	15	15	15
y3 (1+)	9	4	2.9	8.2	7	4.5	12.2	8.1	5.4	15	15	15
y5 (1+)	14.3	2.7	4.9	15	7.6	6.6	20.7	8.1	8.2	15	15	15
y6 (1+)	4.8	2.5	2.7	9	3.9	3.4	10.2	4.6	4.3	15	15	15
y7 (1+)	4.5	2.3	2.4	8.8	5.3	4.3	9.9	5.8	4.9	15	15	15
sum	3.3	1.3	1.3	7.5	4.4	2.6	8.2	4.6	2.9	15	15	15

Angptl3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4016 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Search form

Angptl3

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4016 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments