ATRN, CPTAC-1089 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ATRN	KIAA0548, MGCA

Sequence Length (AA)	Molecular Weight (Da)
1429	158537

Protein Name
Attractin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MVAAAAATEA	RLRRRTAATA	ALAGRSGGPH	WDWDVTRAGR	PGLGAGLRLP
60	70	80	90	100
RLLSPPLRPR	LLLLLLLLSP	PLLLLLLPCE	AEAAAAAAAV	SGSAAAEAKE
110	120	130	140	150
CDRPCVNGGR	CNPGTGQCVC	PAGWVGEQCQ	HCGGRFRLTG	SSGFVTDGPG
160	170	180	190	200
NYKYKTKCTW	LIEGQPNRIM	RLRFNHFATE	CSWDHLYVYD	GDSIYAPLVA
210	220	230	240	250
AFSGLIVPER	DGNETVPEVV	ATSGYALLHF	FSDAAYNLTG	FNITYSFDMC
260	270	280	290	300
PNNCSGRGEC	KISNSSDTVE	CECSENWKGE	ACDIPHCTDN	CGFPHRGICN
310	320	330	340	350
SSDVRGCSCF	SDWQGPGCSV	PVPANQSFWT	REEYSNLKLP	RASHKAVVNG
360	370	380	390	400
NIMWVVGGYM	FNHSDYNMVL	AYDLASREWL	PLNRSVNNVV	VRYGHSLALY
410	420	430	440	450
KDKIYMYGGK	IDSTGNVTNE	LRVFHIHNES	WVLLTPKAKE	QYAVVGHSAH
460	470	480	490	500
IVTLKNGRVV	MLVIFGHCPL	YGYISNVQEY	DLDKNTWSIL	HTQGALVQGG
510	520	530	540	550
YGHSSVYDHR	TRALYVHGGY	KAFSANKYRL	ADDLYRYDVD	TQMWTILKDS
560	570	580	590	600
RFFRYLHTAV	IVSGTMLVFG	GNTHNDTSMS	HGAKCFSSDF	MAYDIACDRW
610	620	630	640	650
SVLPRPDLHH	DVNRFGHSAV	LHNSTMYVFG	GFNSLLLSDI	LVFTSEQCDA
660	670	680	690	700
HRSEAACLAA	GPGIRCVWNT	GSSQCISWAL	ATDEQEEKLK	SECFSKRTLD
710	720	730	740	750
HDRCDQHTDC	YSCTANTNDC	HWCNDHCVPR	NHSCSEGQIS	IFRYENCPKD
760	770	780	790	800
NPMYYCNKKT	SCRSCALDQN	CQWEPRNQEC	IALPENICGI	GWHLVGNSCL
810	820	830	840	850
KITTAKENYD	NAKLFCRNHN	ALLASLTTQK	KVEFVLKQLR	IMQSSQSMSK
860	870	880	890	900
LTLTPWVGLR	KINVSYWCWE	DMSPFTNSLL	QWMPSEPSDA	GFCGILSEPS
910	920	930	940	950
TRGLKAATCI	NPLNGSVCER	PANHSAKQCR	TPCALRTACG	DCTSGSSECM
960	970	980	990	1000
WCSNMKQCVD	SNAYVASFPF	GQCMEWYTMS	TCPPENCSGY	CTCSHCLEQP
1010	1020	1030	1040	1050
GCGWCTDPSN	TGKGKCIEGS	YKGPVKMPSQ	APTGNFYPQP	LLNSSMCLED
1060	1070	1080	1090	1100
SRYNWSFIHC	PACQCNGHSK	CINQSICEKC	ENLTTGKHCE	TCISGFYGDP
1110	1120	1130	1140	1150
TNGGKCQPCK	CNGHASLCNT	NTGKCFCTTK	GVKGDECQLC	EVENRYQGNP
1160	1170	1180	1190	1200
LRGTCYYTLL	IDYQFTFSLS	QEDDRYYTAI	NFVATPDEQN	RDLDMFINAS
1210	1220	1230	1240	1250
KNFNLNITWA	ASFSAGTQAG	EEMPVVSKTN	IKEYKDSFSN	EKFDFRNHPN
1260	1270	1280	1290	1300
ITFFVYVSNF	TWPIKIQIAF	SQHSNFMDLV	QFFVTFFSCF	LSLLLVAAVV
1310	1320	1330	1340	1350
WKIKQSCWAS	RRREQLLREM	QQMASRPFAS	VNVALETDEE	PPDLIGGSIK
1360	1370	1380	1390	1400
TVPKPIALEP	CFGNKAAVLS	VFVRLPRGLG	GIPPPGQSGL	AVASALVDIS
1410	1420	1429
QQMPIVYKEK	SGAVRNRKQQ	PPAQPGTCI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-664 Collapse assay details

Data source: Panorama

Official Gene Symbol: ATRN
Peptide Modified Sequence: IDSTGN[+1.0]VTNELR
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 416
Peptide - Site of Modification: 6
Peptide Start: 411
Peptide End: 422

CPTAC ID: CPTAC-664
Peptide Molecular Mass: 1,318.6365
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	11.3	3.8	3.6	16.4	5.1	3.8	19.9	6.4	5.2	15	15	15
y8 (1+)	10.9	4.2	5.1	11.8	4.4	5.4	16.1	6.1	7.4	15	15	15
y6 (1+)	12.2	4.3	4.8	12.7	4.2	6.2	17.6	6	7.8	15	15	15
sum	11	3.6	4.1	12.3	3.5	4.9	16.5	5	6.4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1089 Collapse assay details

Data source: Panorama

Official Gene Symbol: ATRN
Peptide Sequence: SVNNVVVR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 385
Peptide End: 392

CPTAC ID: non-CPTAC-1089
Peptide Molecular Mass: 885.5032
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers