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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ATRN

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ATRN KIAA0548, MGCA
Sequence Length (AA) Molecular Weight (Da)
1429 158537
Protein Name
Attractin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVAAAAATEA RLRRRTAATA ALAGRSGGPH WDWDVTRAGR PGLGAGLRLP
60 70 80 90 100
RLLSPPLRPR LLLLLLLLSP PLLLLLLPCE AEAAAAAAAV SGSAAAEAKE
110 120 130 140 150
CDRPCVNGGR CNPGTGQCVC PAGWVGEQCQ HCGGRFRLTG SSGFVTDGPG
160 170 180 190 200
NYKYKTKCTW LIEGQPNRIM RLRFNHFATE CSWDHLYVYD GDSIYAPLVA
210 220 230 240 250
AFSGLIVPER DGNETVPEVV ATSGYALLHF FSDAAYNLTG FNITYSFDMC
260 270 280 290 300
PNNCSGRGEC KISNSSDTVE CECSENWKGE ACDIPHCTDN CGFPHRGICN
310 320 330 340 350
SSDVRGCSCF SDWQGPGCSV PVPANQSFWT REEYSNLKLP RASHKAVVNG
360 370 380 390 400
NIMWVVGGYM FNHSDYNMVL AYDLASREWL PLNRSVNNVV VRYGHSLALY
410 420 430 440 450
KDKIYMYGGK IDSTGNVTNE LRVFHIHNES WVLLTPKAKE QYAVVGHSAH
460 470 480 490 500
IVTLKNGRVV MLVIFGHCPL YGYISNVQEY DLDKNTWSIL HTQGALVQGG
510 520 530 540 550
YGHSSVYDHR TRALYVHGGY KAFSANKYRL ADDLYRYDVD TQMWTILKDS
560 570 580 590 600
RFFRYLHTAV IVSGTMLVFG GNTHNDTSMS HGAKCFSSDF MAYDIACDRW
610 620 630 640 650
SVLPRPDLHH DVNRFGHSAV LHNSTMYVFG GFNSLLLSDI LVFTSEQCDA
660 670 680 690 700
HRSEAACLAA GPGIRCVWNT GSSQCISWAL ATDEQEEKLK SECFSKRTLD
710 720 730 740 750
HDRCDQHTDC YSCTANTNDC HWCNDHCVPR NHSCSEGQIS IFRYENCPKD
760 770 780 790 800
NPMYYCNKKT SCRSCALDQN CQWEPRNQEC IALPENICGI GWHLVGNSCL
810 820 830 840 850
KITTAKENYD NAKLFCRNHN ALLASLTTQK KVEFVLKQLR IMQSSQSMSK
860 870 880 890 900
LTLTPWVGLR KINVSYWCWE DMSPFTNSLL QWMPSEPSDA GFCGILSEPS
910 920 930 940 950
TRGLKAATCI NPLNGSVCER PANHSAKQCR TPCALRTACG DCTSGSSECM
960 970 980 990 1000
WCSNMKQCVD SNAYVASFPF GQCMEWYTMS TCPPENCSGY CTCSHCLEQP
1010 1020 1030 1040 1050
GCGWCTDPSN TGKGKCIEGS YKGPVKMPSQ APTGNFYPQP LLNSSMCLED
1060 1070 1080 1090 1100
SRYNWSFIHC PACQCNGHSK CINQSICEKC ENLTTGKHCE TCISGFYGDP
1110 1120 1130 1140 1150
TNGGKCQPCK CNGHASLCNT NTGKCFCTTK GVKGDECQLC EVENRYQGNP
1160 1170 1180 1190 1200
LRGTCYYTLL IDYQFTFSLS QEDDRYYTAI NFVATPDEQN RDLDMFINAS
1210 1220 1230 1240 1250
KNFNLNITWA ASFSAGTQAG EEMPVVSKTN IKEYKDSFSN EKFDFRNHPN
1260 1270 1280 1290 1300
ITFFVYVSNF TWPIKIQIAF SQHSNFMDLV QFFVTFFSCF LSLLLVAAVV
1310 1320 1330 1340 1350
WKIKQSCWAS RRREQLLREM QQMASRPFAS VNVALETDEE PPDLIGGSIK
1360 1370 1380 1390 1400
TVPKPIALEP CFGNKAAVLS VFVRLPRGLG GIPPPGQSGL AVASALVDIS
1410 1420 1429
QQMPIVYKEK SGAVRNRKQQ PPAQPGTCI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-664 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRN
Peptide Modified Sequence
IDSTGN[+1.0]VTNELR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
416
Peptide - Site of Modification
6
Peptide Start
411
Peptide End
422
CPTAC ID
CPTAC-664
Peptide Molecular Mass
1,318.6365
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 11.3 3.8 3.6 16.4 5.1 3.8 19.9 6.4 5.2 15 15 15
y8 (1+) 10.9 4.2 5.1 11.8 4.4 5.4 16.1 6.1 7.4 15 15 15
y6 (1+) 12.2 4.3 4.8 12.7 4.2 6.2 17.6 6 7.8 15 15 15
sum 11 3.6 4.1 12.3 3.5 4.9 16.5 5 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-1089 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATRN
Peptide Sequence
SVNNVVVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
385
Peptide End
392
CPTAC ID
non-CPTAC-1089
Peptide Molecular Mass
885.5032
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 21 12.6 7.3 32.5 11 8.5 38.7 16.7 11.2 14 15 15
y3 (1+) 17.5 6.2 5.2 25.6 8.1 6.5 31 10.2 8.3 15 15 15
y4 (1+) 19.8 5.9 4.5 22.4 6.3 4.6 29.9 8.6 6.4 15 15 15
y5 (1+) 19.3 8 6.1 24.1 8.1 6.8 30.9 11.4 9.1 14 15 15
y6 (1+) 9.6 7.7 7.6 12.4 7.1 6.9 15.7 10.5 10.3 15 15 15
sum 5.1 5.1 4.6 7.7 4.5 4.1 9.2 6.8 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments