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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

BTD

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
BTD N/A
Sequence Length (AA) Molecular Weight (Da)
543 61133
Protein Name
Biotinidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAHAHIQGGR RAKSRFVVCI MSGARSKLAL FLCGCYVVAL GAHTGEESVA
60 70 80 90 100
DHHEAEYYVA AVYEHPSILS LNPLALISRQ EALELMNQNL DIYEQQVMTA
110 120 130 140 150
AQKDVQIIVF PEDGIHGFNF TRTSIYPFLD FMPSPQVVRW NPCLEPHRFN
160 170 180 190 200
DTEVLQRLSC MAIRGDMFLV ANLGTKEPCH SSDPRCPKDG RYQFNTNVVF
210 220 230 240 250
SNNGTLVDRY RKHNLYFEAA FDVPLKVDLI TFDTPFAGRF GIFTCFDILF
260 270 280 290 300
FDPAIRVLRD YKVKHVVYPT AWMNQLPLLA AIEIQKAFAV AFGINVLAAN
310 320 330 340 350
VHHPVLGMTG SGIHTPLESF WYHDMENPKS HLIIAQVAKN PVGLIGAENA
360 370 380 390 400
TGETDPSHSK FLKILSGDPY CEKDAQEVHC DEATKWNVNA PPTFHSEMMY
410 420 430 440 450
DNFTLVPVWG KEGYLHVCSN GLCCYLLYER PTLSKELYAL GVFDGLHTVH
460 470 480 490 500
GTYYIQVCAL VRCGGLGFDT CGQEITEATG IFEFHLWGNF STSYIFPLFL
510 520 530 540 543
TSGMTLEVPD QLGWENDHYF LRKSRLSSGL VTAALYGRLY ERD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-655 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Modified Sequence
FN[+1.0]DTEVLQR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
150
Peptide - Site of Modification
2
Peptide Start
149
Peptide End
157
CPTAC ID
CPTAC-655
Peptide Molecular Mass
1,121.5353
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.2 6.6 8.9 8.8 7.4 9.9 12 9.9 13.3 15 15 15
y7 (1+) 11.8 7.3 10.4 11.5 7.1 9.9 16.5 10.2 14.4 15 15 15
y6 (1+) 11.6 10.6 8.3 9.6 9.7 9.7 15.1 14.4 12.8 15 15 15
sum 7.4 6.9 8.2 6.9 6.7 8.5 10.1 9.6 11.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Assay Details for non-CPTAC-2635 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Sequence
LSSGLVTAALYGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
526
Peptide End
538
CPTAC ID
non-CPTAC-2635
Peptide Molecular Mass
1,306.7245
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.5 7.5 11.3 17 10.3 11.2 21.1 12.7 15.9 15 15 15
y8 (1+) 9.3 7.1 8.8 10.1 10.6 13.2 13.7 12.8 15.9 15 15 15
b4 (1+) 10.3 8.1 11.4 13.5 8.9 10.8 17 12 15.7 15 15 15
y7 (1+) 6.9 8 7.3 8.7 7.7 8.1 11.1 11.1 10.9 15 15 15
y5 (1+) 12.1 8.8 7.5 14.5 8.8 6.9 18.9 12.4 10.2 15 15 15
sum 6.1 5.7 5.9 8.9 6.8 7.5 10.8 8.9 9.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-2634 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Sequence
SHLIIAQVAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
330
Peptide End
339
CPTAC ID
non-CPTAC-2634
Peptide Molecular Mass
1,078.6499
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.5 4.8 6.8 13 8.8 12.2 15 10 14 15 15 15
b5 (1+) 12.3 5.6 7.2 18.7 8 8.3 22.4 9.8 11 15 15 15
y3 (1+) 15.8 5.3 6.8 18.1 8.8 10.7 24 10.3 12.7 15 15 15
b3 (1+) 10.9 5.8 7.4 11.6 10.4 11.9 15.9 11.9 14 15 15 15
b4 (1+) 8.5 6.7 7.6 16 11.3 9.7 18.1 13.1 12.3 15 15 15
sum 6 3.9 5.8 12 8.7 9.6 13.4 9.5 11.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments