BTD, CPTAC-2635 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BTD	N/A

Sequence Length (AA)	Molecular Weight (Da)
543	61133

Protein Name
Biotinidase

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAHAHIQGGR	RAKSRFVVCI	MSGARSKLAL	FLCGCYVVAL	GAHTGEESVA
60	70	80	90	100
DHHEAEYYVA	AVYEHPSILS	LNPLALISRQ	EALELMNQNL	DIYEQQVMTA
110	120	130	140	150
AQKDVQIIVF	PEDGIHGFNF	TRTSIYPFLD	FMPSPQVVRW	NPCLEPHRFN
160	170	180	190	200
DTEVLQRLSC	MAIRGDMFLV	ANLGTKEPCH	SSDPRCPKDG	RYQFNTNVVF
210	220	230	240	250
SNNGTLVDRY	RKHNLYFEAA	FDVPLKVDLI	TFDTPFAGRF	GIFTCFDILF
260	270	280	290	300
FDPAIRVLRD	YKVKHVVYPT	AWMNQLPLLA	AIEIQKAFAV	AFGINVLAAN
310	320	330	340	350
VHHPVLGMTG	SGIHTPLESF	WYHDMENPKS	HLIIAQVAKN	PVGLIGAENA
360	370	380	390	400
TGETDPSHSK	FLKILSGDPY	CEKDAQEVHC	DEATKWNVNA	PPTFHSEMMY
410	420	430	440	450
DNFTLVPVWG	KEGYLHVCSN	GLCCYLLYER	PTLSKELYAL	GVFDGLHTVH
460	470	480	490	500
GTYYIQVCAL	VRCGGLGFDT	CGQEITEATG	IFEFHLWGNF	STSYIFPLFL
510	520	530	540	543
TSGMTLEVPD	QLGWENDHYF	LRKSRLSSGL	VTAALYGRLY	ERD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-655 Collapse assay details

Data source: Panorama

Official Gene Symbol: BTD
Peptide Modified Sequence: FN[+1.0]DTEVLQR
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 150
Peptide - Site of Modification: 2
Peptide Start: 149
Peptide End: 157

CPTAC ID: CPTAC-655
Peptide Molecular Mass: 1,121.5353
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	8.2	6.6	8.9	8.8	7.4	9.9	12	9.9	13.3	15	15	15
y7 (1+)	11.8	7.3	10.4	11.5	7.1	9.9	16.5	10.2	14.4	15	15	15
y6 (1+)	11.6	10.6	8.3	9.6	9.7	9.7	15.1	14.4	12.8	15	15	15
sum	7.4	6.9	8.2	6.9	6.7	8.5	10.1	9.6	11.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2635 Collapse assay details

Data source: Panorama

Official Gene Symbol: BTD
Peptide Sequence: LSSGLVTAALYGR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 526
Peptide End: 538

CPTAC ID: non-CPTAC-2635
Peptide Molecular Mass: 1,306.7245
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers