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Overview Data source: UniProt

Official Gene Symbol Other Aliases
BTD N/A
Sequence Length (AA) Molecular Weight (Da)
543 61133
Protein Name
Biotinidase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAHAHIQGGR RAKSRFVVCI MSGARSKLAL FLCGCYVVAL GAHTGEESVA
60 70 80 90 100
DHHEAEYYVA AVYEHPSILS LNPLALISRQ EALELMNQNL DIYEQQVMTA
110 120 130 140 150
AQKDVQIIVF PEDGIHGFNF TRTSIYPFLD FMPSPQVVRW NPCLEPHRFN
160 170 180 190 200
DTEVLQRLSC MAIRGDMFLV ANLGTKEPCH SSDPRCPKDG RYQFNTNVVF
210 220 230 240 250
SNNGTLVDRY RKHNLYFEAA FDVPLKVDLI TFDTPFAGRF GIFTCFDILF
260 270 280 290 300
FDPAIRVLRD YKVKHVVYPT AWMNQLPLLA AIEIQKAFAV AFGINVLAAN
310 320 330 340 350
VHHPVLGMTG SGIHTPLESF WYHDMENPKS HLIIAQVAKN PVGLIGAENA
360 370 380 390 400
TGETDPSHSK FLKILSGDPY CEKDAQEVHC DEATKWNVNA PPTFHSEMMY
410 420 430 440 450
DNFTLVPVWG KEGYLHVCSN GLCCYLLYER PTLSKELYAL GVFDGLHTVH
460 470 480 490 500
GTYYIQVCAL VRCGGLGFDT CGQEITEATG IFEFHLWGNF STSYIFPLFL
510 520 530 540 543
TSGMTLEVPD QLGWENDHYF LRKSRLSSGL VTAALYGRLY ERD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-655 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Modified Sequence
FN[+1.0]DTEVLQR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
150
Peptide - Site of Modification
2
Peptide Start
149
Peptide End
157
CPTAC ID
CPTAC-655
Peptide Molecular Mass
1,121.5353
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 8.2 6.6 8.9 8.8 7.4 9.9 12 9.9 13.3 15 15 15
y7 (1+) 11.8 7.3 10.4 11.5 7.1 9.9 16.5 10.2 14.4 15 15 15
y6 (1+) 11.6 10.6 8.3 9.6 9.7 9.7 15.1 14.4 12.8 15 15 15
sum 7.4 6.9 8.2 6.9 6.7 8.5 10.1 9.6 11.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2635 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Sequence
LSSGLVTAALYGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
526
Peptide End
538
CPTAC ID
non-CPTAC-2635
Peptide Molecular Mass
1,306.7245
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.5 7.5 11.3 17 10.3 11.2 21.1 12.7 15.9 15 15 15
y8 (1+) 9.3 7.1 8.8 10.1 10.6 13.2 13.7 12.8 15.9 15 15 15
b4 (1+) 10.3 8.1 11.4 13.5 8.9 10.8 17 12 15.7 15 15 15
y7 (1+) 6.9 8 7.3 8.7 7.7 8.1 11.1 11.1 10.9 15 15 15
y5 (1+) 12.1 8.8 7.5 14.5 8.8 6.9 18.9 12.4 10.2 15 15 15
sum 6.1 5.7 5.9 8.9 6.8 7.5 10.8 8.9 9.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2634 Collapse assay details

Data source: Panorama

Official Gene Symbol
BTD
Peptide Sequence
SHLIIAQVAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
330
Peptide End
339
CPTAC ID
non-CPTAC-2634
Peptide Molecular Mass
1,078.6499
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 7.5 4.8 6.8 13 8.8 12.2 15 10 14 15 15 15
b5 (1+) 12.3 5.6 7.2 18.7 8 8.3 22.4 9.8 11 15 15 15
y3 (1+) 15.8 5.3 6.8 18.1 8.8 10.7 24 10.3 12.7 15 15 15
b3 (1+) 10.9 5.8 7.4 11.6 10.4 11.9 15.9 11.9 14 15 15 15
b4 (1+) 8.5 6.7 7.6 16 11.3 9.7 18.1 13.1 12.3 15 15 15
sum 6 3.9 5.8 12 8.7 9.6 13.4 9.5 11.2 15 15 15


Additional Resources and Comments