Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

APOH

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
APOH B2G1
Sequence Length (AA) Molecular Weight (Da)
345 38298
Protein Name
Beta-2-glycoprotein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MISPVLILFS SFLCHVAIAG RTCPKPDDLP FSTVVPLKTF YEPGEEITYS
60 70 80 90 100
CKPGYVSRGG MRKFICPLTG LWPINTLKCT PRVCPFAGIL ENGAVRYTTF
110 120 130 140 150
EYPNTISFSC NTGFYLNGAD SAKCTEEGKW SPELPVCAPI ICPPPSIPTF
160 170 180 190 200
ATLRVYKPSA GNNSLYRDTA VFECLPQHAM FGNDTITCTT HGNWTKLPEC
210 220 230 240 250
REVKCPFPSR PDNGFVNYPA KPTLYYKDKA TFGCHDGYSL DGPEEIECTK
260 270 280 290 300
LGNWSAMPSC KASCKVPVKK ATVVYQGERV KIQEKFKNGM LHGDKVSFFC
310 320 330 340 345
KNKEKKCSYT EDAQCIDGTI EVPKCFKEHS SLAFWKTDAS DVKPC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-654 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOH
Peptide Modified Sequence
VYKPSAGN[+1.0]NSLYR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
162
Peptide - Site of Modification
8
Peptide Start
155
Peptide End
167
CPTAC ID
CPTAC-654
Peptide Molecular Mass
1,468.7311
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 2.2 3.4 2.7 2.5 3.9 5.1 3.3 5.2 5.8 15 15 15
y9 (1+) 6.6 3.4 3.2 7.4 4.8 4.6 9.9 5.9 5.6 15 15 15
y8 (1+) 12.2 4.5 6 14.6 4.1 7.3 19 6.1 9.4 15 15 15
sum 2.5 3.3 2.7 2.7 3.9 5 3.7 5.1 5.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-1090 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOH
Peptide Sequence
ATVVYQGER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
271
Peptide End
279
CPTAC ID
non-CPTAC-1090
Peptide Molecular Mass
1,021.5193
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 15.7 8.2 5.8 16 8.3 5.8 22.4 11.7 8.2 15 15 15
y3 (1+) 12 6.2 6 8.2 7.6 7.9 14.5 9.8 9.9 15 15 15
y5 (1+) 7.7 7.3 8.2 8.8 8.5 6.7 11.7 11.2 10.6 15 15 15
y6 (1+) 10.6 8.8 4.8 12.9 11.5 6.4 16.7 14.5 8 15 15 15
y7 (1+) 12.6 7.4 4.6 15.4 7.3 6.5 19.9 10.4 8 15 15 15
sum 7 3.9 2.7 9.7 6.2 3.5 12 7.3 4.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3894 Collapse assay details

Data source: Panorama

Official Gene Symbol
Apoh
Peptide Sequence
YTSFEYPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
97
Peptide End
104
CPTAC ID
non-CPTAC-3894
Peptide Molecular Mass
1,033.4757
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.5 5.9 4.5 7.7 7.5 5.1 9.5 9.5 6.8 15 15 15
y6 (1+) 3.6 4 4.1 6.2 6.3 4.4 7.2 7.5 6 15 15 15
y3 (1+) 6.9 4.9 3.9 16.6 9.4 3.6 18 10.6 5.3 15 15 15
y2 (1+) 5.2 3 3.7 11 7.7 3.6 12.2 8.3 5.2 15 15 15
b2 (1+) 3.8 3.4 1.7 5.8 4.9 2.3 6.9 6 2.9 15 15 15
sum 2.7 1.7 2.2 9.4 5 2.3 9.8 5.3 3.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments