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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Apoh B2gp1
Sequence Length (AA) Molecular Weight (Da)
345 38619
Protein Name
Beta-2-glycoprotein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVSPVLALFS AFLCHVAIAG RICPKPDDLP FATVVPLKTS YDPGEQIVYS
60 70 80 90 100
CKPGYVSRGG MRRFTCPLTG MWPINTLRCV PRVCPFAGIL ENGIVRYTSF
110 120 130 140 150
EYPKNISFAC NPGFFLNGTS SSKCTEEGKW SPDIPACARI TCPPPPVPKF
160 170 180 190 200
ALLKDYRPSA GNNSLYQDTV VFKCLPHFAM IGNDTVMCTE QGNWTRLPEC
210 220 230 240 250
LEVKCPFPPR PENGYVNYPA KPVLLYKDKA TFGCHETYKL DGPEEAECTK
260 270 280 290 300
TGTWSFLPTC RESCKLPVKK ATVLYQGMRV KIQEQFKNGM MHGDKIHFYC
310 320 330 340 345
KNKEKKCSYT VEAHCRDGTI EIPSCFKEHS SLAFWKTDAS ELTPC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-654 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOH
Peptide Modified Sequence
VYKPSAGN[+1.0]NSLYR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
162
Peptide - Site of Modification
8
Peptide Start
155
Peptide End
167
CPTAC ID
CPTAC-654
Peptide Molecular Mass
1,468.7311
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 2.2 3.4 2.7 2.5 3.9 5.1 3.3 5.2 5.8 15 15 15
y9 (1+) 6.6 3.4 3.2 7.4 4.8 4.6 9.9 5.9 5.6 15 15 15
y8 (1+) 12.2 4.5 6 14.6 4.1 7.3 19 6.1 9.4 15 15 15
sum 2.5 3.3 2.7 2.7 3.9 5 3.7 5.1 5.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1090 Collapse assay details

Data source: Panorama

Official Gene Symbol
APOH
Peptide Sequence
ATVVYQGER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
271
Peptide End
279
CPTAC ID
non-CPTAC-1090
Peptide Molecular Mass
1,021.5193
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 15.7 8.2 5.8 16 8.3 5.8 22.4 11.7 8.2 15 15 15
y3 (1+) 12 6.2 6 8.2 7.6 7.9 14.5 9.8 9.9 15 15 15
y5 (1+) 7.7 7.3 8.2 8.8 8.5 6.7 11.7 11.2 10.6 15 15 15
y6 (1+) 10.6 8.8 4.8 12.9 11.5 6.4 16.7 14.5 8 15 15 15
y7 (1+) 12.6 7.4 4.6 15.4 7.3 6.5 19.9 10.4 8 15 15 15
sum 7 3.9 2.7 9.7 6.2 3.5 12 7.3 4.4 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3894 Collapse assay details

Data source: Panorama

Official Gene Symbol
Apoh
Peptide Sequence
YTSFEYPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
97
Peptide End
104
CPTAC ID
non-CPTAC-3894
Peptide Molecular Mass
1,033.4757
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.5 5.9 4.5 7.7 7.5 5.1 9.5 9.5 6.8 15 15 15
y6 (1+) 3.6 4 4.1 6.2 6.3 4.4 7.2 7.5 6 15 15 15
y3 (1+) 6.9 4.9 3.9 16.6 9.4 3.6 18 10.6 5.3 15 15 15
y2 (1+) 5.2 3 3.7 11 7.7 3.6 12.2 8.3 5.2 15 15 15
b2 (1+) 3.8 3.4 1.7 5.8 4.9 2.3 6.9 6 2.9 15 15 15
sum 2.7 1.7 2.2 9.4 5 2.3 9.8 5.3 3.2 15 15 15

Additional Resources and Comments