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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C8A

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C8A N/A
Sequence Length (AA) Molecular Weight (Da)
584 65163
Protein Name
Complement component C8 alpha chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFAVVFFILS LMTCQPGVTA QEKVNQRVRR AATPAAVTCQ LSNWSEWTDC
60 70 80 90 100
FPCQDKKYRH RSLLQPNKFG GTICSGDIWD QASCSSSTTC VRQAQCGQDF
110 120 130 140 150
QCKETGRCLK RHLVCNGDQD CLDGSDEDDC EDVRAIDEDC SQYEPIPGSQ
160 170 180 190 200
KAALGYNILT QEDAQSVYDA SYYGGQCETV YNGEWRELRY DSTCERLYYG
210 220 230 240 250
DDEKYFRKPY NFLKYHFEAL ADTGISSEFY DNANDLLSKV KKDKSDSFGV
260 270 280 290 300
TIGIGPAGSP LLVGVGVSHS QDTSFLNELN KYNEKKFIFT RIFTKVQTAH
310 320 330 340 350
FKMRKDDIML DEGMLQSLME LPDQYNYGMY AKFINDYGTH YITSGSMGGI
360 370 380 390 400
YEYILVIDKA KMESLGITSR DITTCFGGSL GIQYEDKINV GGGLSGDHCK
410 420 430 440 450
KFGGGKTERA RKAMAVEDII SRVRGGSSGW SGGLAQNRST ITYRSWGRSL
460 470 480 490 500
KYNPVVIDFE MQPIHEVLRH TSLGPLEAKR QNLRRALDQY LMEFNACRCG
510 520 530 540 550
PCFNNGVPIL EGTSCRCQCR LGSLGAACEQ TQTEGAKADG SWSCWSSWSV
560 570 580 584
CRAGIQERRR ECDNPAPQNG GASCPGRKVQ TQAC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-667 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8A
Peptide Modified Sequence
GGSSGWSGGLAQN[+1.0]R
Modification Type
Deamidated (NQ)
Protein - Site of Modification
437
Peptide - Site of Modification
13
Peptide Start
425
Peptide End
438
CPTAC ID
CPTAC-667
Peptide Molecular Mass
1,333.6011
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 3.9 5.8 4.9 5.3 6.3 5.1 6.6 8.6 7.1 15 15 15
y8 (1+) 3.3 3.3 2.8 4.6 4.7 2.7 5.7 5.7 3.9 15 15 15
y7 (1+) 4.8 4.1 4.2 4.9 6 3.6 6.9 7.3 5.5 15 15 15
sum 3.6 3.6 3.1 4.3 5 2.6 5.6 6.2 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3570 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8a
Peptide Sequence
HTNLGPLETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
470
Peptide End
479
CPTAC ID
non-CPTAC-3570
Peptide Molecular Mass
1,108.5877
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK 		CPTAC-649:
AFM.
DIENFN[+1.0]STQK 		CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK 		CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR 		CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR 		CPTAC-655:
BTD.
FN[+1.0]DTEVLQR 		CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK 		CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR 		CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR 		CPTAC-661:
CLU.
EDALN[+1.0]ETR 		CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK 		CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR 		CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR 		CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R 		CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR 		CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR 		CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK 		CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR 		CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR 		CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK 		CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR 		CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK 		CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR 		CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK 		CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR 		CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK 		CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK 		CPTAC-688:
LAMP2.
LN[+1.0]SSTIK 		CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 14.5 5.8 4.8 16.2 9.2 6.3 21.7 10.9 7.9 15 15 15
b3 (1+) 14.4 5.8 3.7 15.5 11.1 6.9 21.2 12.5 7.8 15 15 15
y5 (2+) 14 4.5 4.1 16.4 12.3 5.5 21.6 13.1 6.9 15 15 15
y5 (1+) 7.4 4.9 4.4 6.3 7.5 10.7 9.7 9 11.6 15 15 15
b5 (2+) 11.7 6.4 3.9 10.6 11.9 8.1 15.8 13.5 9 15 15 15
sum 5.8 3 1.6 6 9.7 6.6 8.3 10.2 6.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments