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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C8a N/A
Sequence Length (AA) Molecular Weight (Da)
587 66080
Protein Name
Complement component C8 alpha chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MFVVAFFGLS LVAWHPGVTA QEKVNQRVTR AVTPQAVSCQ LSDWYKWTDC
60 70 80 90 100
FPCQDKKYRY RSLLQPSKFG GTICSGDIWD EASCDSPTPC LRQAQCGQDF
110 120 130 140 150
QCRETGRCLK RHLVCNGDND CLDGSDESDC EDVRVTEDDC HQYEPIPGSE
160 170 180 190 200
RAALGYNILT QEEAQSVYDA KYYGGQCETV YNGDWRKLRY DPTCERLYYG
210 220 230 240 250
EDEKYFRKPY NFLKYHFEAL ADTSISSEFY DDANDLFFHI KNGKSHSAGV
260 270 280 290 300
TVGVAPVKSP VSIEVTGSGS KASSFLNKLN KYNEKRYGFM RVSTKIQTAQ
310 320 330 340 350
FKMRRNNIVL DEGMLQSLME LPEQFNYGMY AKFINDYGTH YITSGTMGGI
360 370 380 390 400
YEYVMVLDKE KMKTEGTTVD EVQKCIGGGI GIGIKDSTIE GVGISGEFCE
410 420 430 440 450
NSGDGDRDIR KKITGVEDII SRVQGGSSVW GSVLTHNSSA ITYQSWGRSL
460 470 480 490 500
KYNPVVIDFE MQPIYQLLRH TNLGPLETKR QNLRRALDQY LMEFNACRCG
510 520 530 540 550
PCFNNGEPIL DGTNCRCQCS MGRQGLACER TVIEGLKDFK AAGHWSCWSS
560 570 580 587
WSECRGGSQE RRRQCNNPPP KNGGTPCLGR NLQTQAC

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-667 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8A
Peptide Modified Sequence
GGSSGWSGGLAQN[+1.0]R
Modification Type
Deamidated (NQ)
Protein - Site of Modification
437
Peptide - Site of Modification
13
Peptide Start
425
Peptide End
438
CPTAC ID
CPTAC-667
Peptide Molecular Mass
1,333.6011
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 3.9 5.8 4.9 5.3 6.3 5.1 6.6 8.6 7.1 15 15 15
y8 (1+) 3.3 3.3 2.8 4.6 4.7 2.7 5.7 5.7 3.9 15 15 15
y7 (1+) 4.8 4.1 4.2 4.9 6 3.6 6.9 7.3 5.5 15 15 15
sum 3.6 3.6 3.1 4.3 5 2.6 5.6 6.2 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3570 Collapse assay details

Data source: Panorama

Official Gene Symbol
C8a
Peptide Sequence
HTNLGPLETK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
470
Peptide End
479
CPTAC ID
non-CPTAC-3570
Peptide Molecular Mass
1,108.5877
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 14.5 5.8 4.8 16.2 9.2 6.3 21.7 10.9 7.9 15 15 15
b3 (1+) 14.4 5.8 3.7 15.5 11.1 6.9 21.2 12.5 7.8 15 15 15
y5 (2+) 14 4.5 4.1 16.4 12.3 5.5 21.6 13.1 6.9 15 15 15
y5 (1+) 7.4 4.9 4.4 6.3 7.5 10.7 9.7 9 11.6 15 15 15
b5 (2+) 11.7 6.4 3.9 10.6 11.9 8.1 15.8 13.5 9 15 15 15
sum 5.8 3 1.6 6 9.7 6.6 8.3 10.2 6.8 15 15 15


Additional Resources and Comments