C8a, CPTAC-3570 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
C8a	N/A

Sequence Length (AA)	Molecular Weight (Da)
587	66080

Protein Name
Complement component C8 alpha chain

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MFVVAFFGLS	LVAWHPGVTA	QEKVNQRVTR	AVTPQAVSCQ	LSDWYKWTDC
60	70	80	90	100
FPCQDKKYRY	RSLLQPSKFG	GTICSGDIWD	EASCDSPTPC	LRQAQCGQDF
110	120	130	140	150
QCRETGRCLK	RHLVCNGDND	CLDGSDESDC	EDVRVTEDDC	HQYEPIPGSE
160	170	180	190	200
RAALGYNILT	QEEAQSVYDA	KYYGGQCETV	YNGDWRKLRY	DPTCERLYYG
210	220	230	240	250
EDEKYFRKPY	NFLKYHFEAL	ADTSISSEFY	DDANDLFFHI	KNGKSHSAGV
260	270	280	290	300
TVGVAPVKSP	VSIEVTGSGS	KASSFLNKLN	KYNEKRYGFM	RVSTKIQTAQ
310	320	330	340	350
FKMRRNNIVL	DEGMLQSLME	LPEQFNYGMY	AKFINDYGTH	YITSGTMGGI
360	370	380	390	400
YEYVMVLDKE	KMKTEGTTVD	EVQKCIGGGI	GIGIKDSTIE	GVGISGEFCE
410	420	430	440	450
NSGDGDRDIR	KKITGVEDII	SRVQGGSSVW	GSVLTHNSSA	ITYQSWGRSL
460	470	480	490	500
KYNPVVIDFE	MQPIYQLLRH	TNLGPLETKR	QNLRRALDQY	LMEFNACRCG
510	520	530	540	550
PCFNNGEPIL	DGTNCRCQCS	MGRQGLACER	TVIEGLKDFK	AAGHWSCWSS
560	570	580	587
WSECRGGSQE	RRRQCNNPPP	KNGGTPCLGR	NLQTQAC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-667 Collapse assay details

Data source: Panorama

Official Gene Symbol: C8A
Peptide Modified Sequence: GGSSGWSGGLAQN[+1.0]R
Modification Type: Deamidated (NQ)
Protein - Site of Modification: 437
Peptide - Site of Modification: 13
Peptide Start: 425
Peptide End: 438

CPTAC ID: CPTAC-667
Peptide Molecular Mass: 1,333.6011
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive (ThermoFisher)
Internal Standard: synthetic peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing: Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions: 300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate: 0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	3.9	5.8	4.9	5.3	6.3	5.1	6.6	8.6	7.1	15	15	15
y8 (1+)	3.3	3.3	2.8	4.6	4.7	2.7	5.7	5.7	3.9	15	15	15
y7 (1+)	4.8	4.1	4.2	4.9	6	3.6	6.9	7.3	5.5	15	15	15
sum	3.6	3.6	3.1	4.3	5	2.6	5.6	6.2	4	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3570 Collapse assay details

Data source: Panorama

Official Gene Symbol: C8a
Peptide Sequence: HTNLGPLETK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 470
Peptide End: 479

CPTAC ID: non-CPTAC-3570
Peptide Molecular Mass: 1,108.5877
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.