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Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD44 LHR,MDU2,MDU3,MIC4
Sequence Length (AA) Molecular Weight (Da)
742 81538
Protein Name
CD44 antigen
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDKFWWHAAW GLCLVPLSLA QIDLNITCRF AGVFHVEKNG RYSISRTEAA
60 70 80 90 100
DLCKAFNSTL PTMAQMEKAL SIGFETCRYG FIEGHVVIPR IHPNSICAAN
110 120 130 140 150
NTGVYILTSN TSQYDTYCFN ASAPPEEDCT SVTDLPNAFD GPITITIVNR
160 170 180 190 200
DGTRYVQKGE YRTNPEDIYP SNPTDDDVSS GSSSERSSTS GGYIFYTFST
210 220 230 240 250
VHPIPDEDSP WITDSTDRIP ATTLMSTSAT ATETATKRQE TWDWFSWLFL
260 270 280 290 300
PSESKNHLHT TTQMAGTSSN TISAGWEPNE ENEDERDRHL SFSGSGIDDD
310 320 330 340 350
EDFISSTIST TPRAFDHTKQ NQDWTQWNPS HSNPEVLLQT TTRMTDVDRN
360 370 380 390 400
GTTAYEGNWN PEAHPPLIHH EHHEEEETPH STSTIQATPS STTEETATQK
410 420 430 440 450
EQWFGNRWHE GYRQTPKEDS HSTTGTAAAS AHTSHPMQGR TTPSPEDSSW
460 470 480 490 500
TDFFNPISHP MGRGHQAGRR MDMDSSHSIT LQPTANPNTG LVEDLDRTGP
510 520 530 540 550
LSMTTQQSNS QSFSTSHEGL EEDKDHPTTS TLTSSNRNDV TGGRRDPNHS
560 570 580 590 600
EGSTTLLEGY TSHYPHTKES RTFIPVTSAK TGSFGVTAVT VGDSNSNVNR
610 620 630 640 650
SLSGDQDTFH PSGGSHTTHG SESDGHSHGS QEGGANTTSG PIRTPQIPEW
660 670 680 690 700
LIILASLLAL ALILAVCIAV NSRRRCGQKK KLVINSGNGA VEDRKPSGLN
710 720 730 740 742
GEASKSQEMV HLVNKESSET PDQFMTADET RNLQNVDMKI GV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-692 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD44
Peptide Sequence
TFIPVTSAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
572
Peptide End
580
CPTAC ID
CPTAC-692
Peptide Molecular Mass
962.5437
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614


Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK
CPTAC-649:
AFM.
DIENFN[+1.0]STQK
CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK
CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR
CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR
CPTAC-655:
BTD.
FN[+1.0]DTEVLQR
CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK
CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR
CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR
CPTAC-661:
CLU.
EDALN[+1.0]ETR
CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK
CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR
CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR
CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R
CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR
CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR
CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK
CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR
CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR
CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK
CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR
CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK
CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR
CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK
CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR
CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK
CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK
CPTAC-688:
LAMP2.
LN[+1.0]SSTIK
CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 82.4 22.5 12.7 64.3 29.4 13.6 104.5 37 18.6 15 15 15
y6 (1+) 22.3 4.2 2.2 29 4.9 3.6 36.6 6.5 4.2 15 15 15
y7 (1+) 24.1 4.8 2.7 29.4 6 3.9 38 7.7 4.7 15 15 15
sum 17.5 3.3 1.9 21.1 4.7 3.4 27.4 5.7 3.9 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-668 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD44
Peptide Modified Sequence
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
Modification Type
Deamidated (NQ), Oxidation (M), Oxidation (M)
Protein - Site of Modification
57, 63, 66
Peptide - Site of Modification
3, 9, 12
Peptide Start
55
Peptide End
68
CPTAC ID
CPTAC-668
Peptide Molecular Mass
1,600.7113
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK
CPTAC-649:
AFM.
DIENFN[+1.0]STQK
CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK
CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR
CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR
CPTAC-655:
BTD.
FN[+1.0]DTEVLQR
CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK
CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR
CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR
CPTAC-661:
CLU.
EDALN[+1.0]ETR
CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK
CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR
CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR
CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R
CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR
CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR
CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK
CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR
CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR
CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK
CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR
CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK
CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR
CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK
CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR
CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK
CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK
CPTAC-688:
LAMP2.
LN[+1.0]SSTIK
CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.5 14.1 18.7 11.8 15.3 20.2 17.2 20.8 27.5 15 15 15
y8 (1+) 4.8 9.3 5.6 6.2 8.4 5.5 7.8 12.5 7.8 15 15 15
y8 (2+) 19.6 27 15.2 18 27.9 13.1 26.6 38.8 20.1 15 15 15
sum 4.7 9.6 7.2 5.7 8.5 6.4 7.4 12.8 9.6 15 15 15

Additional Resources and Comments