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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

CD44

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CD44 LHR, MDU2, MDU3, MIC4
Sequence Length (AA) Molecular Weight (Da)
742 81538
Protein Name
CD44 antigen
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDKFWWHAAW GLCLVPLSLA QIDLNITCRF AGVFHVEKNG RYSISRTEAA
60 70 80 90 100
DLCKAFNSTL PTMAQMEKAL SIGFETCRYG FIEGHVVIPR IHPNSICAAN
110 120 130 140 150
NTGVYILTSN TSQYDTYCFN ASAPPEEDCT SVTDLPNAFD GPITITIVNR
160 170 180 190 200
DGTRYVQKGE YRTNPEDIYP SNPTDDDVSS GSSSERSSTS GGYIFYTFST
210 220 230 240 250
VHPIPDEDSP WITDSTDRIP ATTLMSTSAT ATETATKRQE TWDWFSWLFL
260 270 280 290 300
PSESKNHLHT TTQMAGTSSN TISAGWEPNE ENEDERDRHL SFSGSGIDDD
310 320 330 340 350
EDFISSTIST TPRAFDHTKQ NQDWTQWNPS HSNPEVLLQT TTRMTDVDRN
360 370 380 390 400
GTTAYEGNWN PEAHPPLIHH EHHEEEETPH STSTIQATPS STTEETATQK
410 420 430 440 450
EQWFGNRWHE GYRQTPKEDS HSTTGTAAAS AHTSHPMQGR TTPSPEDSSW
460 470 480 490 500
TDFFNPISHP MGRGHQAGRR MDMDSSHSIT LQPTANPNTG LVEDLDRTGP
510 520 530 540 550
LSMTTQQSNS QSFSTSHEGL EEDKDHPTTS TLTSSNRNDV TGGRRDPNHS
560 570 580 590 600
EGSTTLLEGY TSHYPHTKES RTFIPVTSAK TGSFGVTAVT VGDSNSNVNR
610 620 630 640 650
SLSGDQDTFH PSGGSHTTHG SESDGHSHGS QEGGANTTSG PIRTPQIPEW
660 670 680 690 700
LIILASLLAL ALILAVCIAV NSRRRCGQKK KLVINSGNGA VEDRKPSGLN
710 720 730 740 742
GEASKSQEMV HLVNKESSET PDQFMTADET RNLQNVDMKI GV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-692 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD44
Peptide Sequence
TFIPVTSAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
572
Peptide End
580
CPTAC ID
CPTAC-692
Peptide Molecular Mass
962.5437
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
plasma
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Schoenherr RM, Saul RG, Whiteaker JR, Yan P, Whiteley GR, Paulovich AG. Mol Cell Proteomics. 2015 Feb;14(2):382-98. doi: 10.1074/mcp.O114.043133. Epub 2014 Dec 15. PMID: 25512614

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 Qtrap
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Eksigent NanoLC 2D
Column Packing
ReproSil-Pur C18-AQ 3 um
Column Dimensions
100 mm x 75 um ID
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 82.4 22.5 12.7 64.3 29.4 13.6 104.5 37 18.6 15 15 15
y6 (1+) 22.3 4.2 2.2 29 4.9 3.6 36.6 6.5 4.2 15 15 15
y7 (1+) 24.1 4.8 2.7 29.4 6 3.9 38 7.7 4.7 15 15 15
sum 17.5 3.3 1.9 21.1 4.7 3.4 27.4 5.7 3.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-6214 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD44
Peptide Sequence
YGFIEGHVVIPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
79
Peptide End
90
CPTAC ID
CPTAC-6214
Peptide Molecular Mass
1,385.7456
Species
Homo Sapiens
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
Frozen tissue
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 5500
Internal Standard
Extended heavy peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent
Column Packing
Reprosil C18, 3um
Column Dimensions
75µm x 15cm
Flow Rate
0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 4.6 3.8 2.7 9.1 8.1 3.7 10.2 8.9 4.6 15 15 15
y5 (1+) 4.6 3.3 3.7 6.4 5.7 6.8 7.9 6.6 7.7 15 15 15
y4 (1+) 4.3 3.3 2.9 8.5 5.5 6.5 9.5 6.4 7.1 15 15 15
y9 (2+) 4.9 3.5 4.2 7.9 6.3 10.1 9.3 7.2 10.9 15 15 15
sum 4.3 2.7 3.1 6.9 5.8 6.7 8.1 6.4 7.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
View in CPTC Antibody Portal
Comments

Assay Details for CPTAC-668 Collapse assay details

Data source: Panorama

Official Gene Symbol
CD44
Peptide Modified Sequence
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
Modification Type
Deamidated (NQ), Oxidation (M), Oxidation (M)
Protein - Site of Modification
57, 63, 66
Peptide - Site of Modification
3, 9, 12
Peptide Start
55
Peptide End
68
CPTAC ID
CPTAC-668
Peptide Molecular Mass
1,600.7113
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
Peptide Immunoaffinity
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.5 14.1 18.7 11.8 15.3 20.2 17.2 20.8 27.5 15 15 15
y8 (1+) 4.8 9.3 5.6 6.2 8.4 5.5 7.8 12.5 7.8 15 15 15
y8 (2+) 19.6 27 15.2 18 27.9 13.1 26.6 38.8 20.1 15 15 15
sum 4.7 9.6 7.2 5.7 8.5 6.4 7.4 12.8 9.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments