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Overview Data source: UniProt

Official Gene Symbol Other Aliases
KLKB1 KLK3
Sequence Length (AA) Molecular Weight (Da)
638 71370
Protein Name
Plasma kallikrein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MILFKQATYF ISLFATVSCG CLTQLYENAF FRGGDVASMY TPNAQYCQMR
60 70 80 90 100
CTFHPRCLLF SFLPASSIND MEKRFGCFLK DSVTGTLPKV HRTGAVSGHS
110 120 130 140 150
LKQCGHQISA CHRDIYKGVD MRGVNFNVSK VSSVEECQKR CTNNIRCQFF
160 170 180 190 200
SYATQTFHKA EYRNNCLLKY SPGGTPTAIK VLSNVESGFS LKPCALSEIG
210 220 230 240 250
CHMNIFQHLA FSDVDVARVL TPDAFVCRTI CTYHPNCLFF TFYTNVWKIE
260 270 280 290 300
SQRNVCLLKT SESGTPSSST PQENTISGYS LLTCKRTLPE PCHSKIYPGV
310 320 330 340 350
DFGGEELNVT FVKGVNVCQE TCTKMIRCQF FTYSLLPEDC KEEKCKCFLR
360 370 380 390 400
LSMDGSPTRI AYGTQGSSGY SLRLCNTGDN SVCTTKTSTR IVGGTNSSWG
410 420 430 440 450
EWPWQVSLQV KLTAQRHLCG GSLIGHQWVL TAAHCFDGLP LQDVWRIYSG
460 470 480 490 500
ILNLSDITKD TPFSQIKEII IHQNYKVSEG NHDIALIKLQ APLNYTEFQK
510 520 530 540 550
PICLPSKGDT STIYTNCWVT GWGFSKEKGE IQNILQKVNI PLVTNEECQK
560 570 580 590 600
RYQDYKITQR MVCAGYKEGG KDACKGDSGG PLVCKHNGMW RLVGITSWGE
610 620 630 638
GCARREQPGV YTKVAEYMDW ILEKTQSSDG KAQMQSPA

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-682 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLKB1
Peptide Modified Sequence
IYPGVDFGGEELN[+1.0]VTFVK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
308
Peptide - Site of Modification
13
Peptide Start
296
Peptide End
313
CPTAC ID
CPTAC-682
Peptide Molecular Mass
1,983.9829
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK
CPTAC-649:
AFM.
DIENFN[+1.0]STQK
CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK
CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR
CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR
CPTAC-655:
BTD.
FN[+1.0]DTEVLQR
CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK
CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR
CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR
CPTAC-661:
CLU.
EDALN[+1.0]ETR
CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK
CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR
CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR
CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R
CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR
CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR
CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK
CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR
CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR
CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK
CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR
CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK
CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR
CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK
CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR
CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK
CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK
CPTAC-688:
LAMP2.
LN[+1.0]SSTIK
CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (1+) 9.6 3.3 2.7 15.6 3 2.3 18.3 4.5 3.5 15 15 15
y11 (1+) 5.1 2.8 2.8 13.3 3.3 2.7 14.2 4.3 3.9 15 15 15
y16 (2+) 6.2 2.6 2.8 14.6 2.6 2.9 15.9 3.7 4 15 15 15
sum 5.3 2 2.3 13.8 2.2 2.2 14.8 3 3.2 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-683 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLKB1
Peptide Modified Sequence
IYSGILN[+1.0]LSDITK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
453
Peptide - Site of Modification
7
Peptide Start
447
Peptide End
459
CPTAC ID
CPTAC-683
Peptide Molecular Mass
1,436.7762
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N-linked glycopeptide solid phase extraction
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK
CPTAC-649:
AFM.
DIENFN[+1.0]STQK
CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK
CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR
CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR
CPTAC-655:
BTD.
FN[+1.0]DTEVLQR
CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK
CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR
CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR
CPTAC-661:
CLU.
EDALN[+1.0]ETR
CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK
CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR
CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR
CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R
CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR
CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR
CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK
CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR
CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR
CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK
CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR
CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK
CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR
CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK
CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR
CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK
CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK
CPTAC-688:
LAMP2.
LN[+1.0]SSTIK
CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 3.6 2.9 4.6 3.9 2.7 4.3 5.3 4 6.3 15 15 15
y8 (1+) 5.5 4.5 4.7 5.3 3.3 4.1 7.6 5.6 6.2 15 15 15
y7 (1+) 4.9 4.6 5.6 5.2 4.1 5.3 7.1 6.2 7.7 15 15 15
sum 3.1 3.7 4.5 2.8 3 4.2 4.2 4.8 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5603 Collapse assay details

Data source: Panorama

Official Gene Symbol
Klkb1
Peptide Sequence
TGAISGHSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
93
Peptide End
102
CPTAC ID
non-CPTAC-5603
Peptide Molecular Mass
969.5243
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Assay Multiplexing Expand assay panel

Johns Hopkins University-A

CPTAC-648:
APMAP.
AGPN[+1.0]GTLFVADAYK
CPTAC-649:
AFM.
DIENFN[+1.0]STQK
CPTAC-650:
AFM.
FN[+1.0]ETTEK
CPTAC-651:
SERPINA1.
YLGN[+1.0]ATAIFFLPDEGK
CPTAC-652:
APOB.
FN[+1.0]SSYLQGTNQITGR
CPTAC-653:
APOB.
YDFN[+1.0]SSM[+16.0]LYSTAK
CPTAC-654:
APOH.
VYKPSAGN[+1.0]NSLYR
CPTAC-655:
BTD.
FN[+1.0]DTEVLQR
CPTAC-656:
CDH13.
IN[+1.0]NTHALVSLLQNLNK
CPTAC-657:
CPB2.
QVHFFVN[+1.0]ASDVDNVK
CPTAC-658:
CTSD.
GSLSYLN[+1.0]VTR
CPTAC-659:
JCHAIN.
EN[+1.0]ISDPTSPLR
CPTAC-660:
CP.
EHEGAIYPDN[+1.0]TTDFQR
CPTAC-661:
CLU.
EDALN[+1.0]ETR
CPTAC-662:
CLU.
LAN[+1.0]LTQGEDQYYLR
CPTAC-663:
DKFZp779M0311.
QSVPAHFVALN[+1.0]GSK
CPTAC-664:
ATRN.
IDSTGN[+1.0]VTNELR
CPTAC-665:
C4A.
FSDGLESN[+1.0]SSTQFEVK
CPTAC-666:
C4A.
GLN[+1.0]VTLSSTGR
CPTAC-667:
C8A.
GGSSGWSGGLAQN[+1.0]R
CPTAC-668:
CD44.
AFN[+1.0]STLPTM[+16.0]AQM[+16.0]EK
CPTAC-669:
IGHG1.
EEQYN[+1.0]STYR
CPTAC-670:
LGALS3BP.
ALGFEN[+1.0]ATQALGR
CPTAC-671:
HP.
VVLHPN[+1.0]YSQVDIGLIK
CPTAC-672:
SERPINA4.
FLN[+1.0]DTM[+16.0]AVYEAK
CPTAC-673:
IGHG4.
EEQFN[+1.0]STYR
CPTAC-674:
IGHA2.
TPLTAN[+1.0]ITK
CPTAC-675:
IGHG2.
EEQFN[+1.0]STFR
CPTAC-676:
ITIH2.
GAFISN[+1.0]FSM[+16.0]TVDGK
CPTAC-677:
ICAM2.
AAPAPQEATATFN[+1.0]STADR
CPTAC-678:
LRG1.
LPPGLLAN[+1.0]FTLLR
CPTAC-679:
LYVE1.
ANQQLN[+1.0]FTEAK
CPTAC-680:
TIMP1.
FVGTPEVN[+1.0]QTTLYQR
CPTAC-681:
LCN2.
SYN[+1.0]VTSVLFR
CPTAC-682:
KLKB1.
IYPGVDFGGEELN[+1.0]VTFVK
CPTAC-683:
KLKB1.
IYSGILN[+1.0]LSDITK
CPTAC-684:
SERPING1.
DTFVN[+1.0]ASR
CPTAC-685:
PIGR.
VPGN[+1.0]VTAVLGETLK
CPTAC-686:
PTGDS.
SVVAPATDGGLN[+1.0]LTSTFLR
CPTAC-687:
KNG1.
LNAENN[+1.0]ATFYFK
CPTAC-688:
LAMP2.
LN[+1.0]SSTIK
CPTAC-689:
LAMP2.
VQPFN[+1.0]VTQGK
CPTAC-690:
VTN.
N[+1.0]GSLFAFR

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 15 8.2 5.7 19 10.2 6.4 24.2 13.1 8.6 15 15 15
y7 (2+) 18.6 6.2 5.7 20.5 12 8.2 27.7 13.5 10 15 15 15
y8 (2+) 9.7 8.7 7.3 10.9 10.4 6.8 14.6 13.6 10 15 15 15
y9 (2+) 7.5 8.3 5.6 10.2 10.2 6.5 12.7 13.2 8.6 15 15 15
y6 (1+) 7.6 4.8 6.2 8.3 6.7 6.8 11.3 8.2 9.2 15 15 15
sum 6.7 4.2 3.2 8.5 7.3 4.8 10.8 8.4 5.8 15 15 15

Additional Resources and Comments