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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Klkb1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Klkb1 Klk3, Pk
Sequence Length (AA) Molecular Weight (Da)
638 71383
Protein Name
Plasma kallikrein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MILFNRVGYF VSLFATVSCG CMTQLYKNTF FRGGDLAAIY TPDAQYCQKM
60 70 80 90 100
CTFHPRCLLF SFLAVTPPKE TNKRFGCFMK ESITGTLPRI HRTGAISGHS
110 120 130 140 150
LKQCGHQISA CHRDIYKGLD MRGSNFNISK TDNIEECQKL CTNNFHCQFF
160 170 180 190 200
TYATSAFYRP EYRKKCLLKH SASGTPTSIK SADNLVSGFS LKSCALSEIG
210 220 230 240 250
CPMDIFQHSA FADLNVSQVI TPDAFVCRTI CTFHPNCLFF TFYTNEWETE
260 270 280 290 300
SQRNVCFLKT SKSGRPSPPI PQENAISGYS LLTCRKTRPE PCHSKIYSGV
310 320 330 340 350
DFEGEELNVT FVQGADVCQE TCTKTIRCQF FIYSLLPQDC KEEGCKCSLR
360 370 380 390 400
LSTDGSPTRI TYGMQGSSGY SLRLCKLVDS PDCTTKINAR IVGGTNASLG
410 420 430 440 450
EWPWQVSLQV KLVSQTHLCG GSIIGRQWVL TAAHCFDGIP YPDVWRIYGG
460 470 480 490 500
ILSLSEITKE TPSSRIKELI IHQEYKVSEG NYDIALIKLQ TPLNYTEFQK
510 520 530 540 550
PICLPSKADT NTIYTNCWVT GWGYTKEQGE TQNILQKATI PLVPNEECQK
560 570 580 590 600
KYRDYVINKQ MICAGYKEGG TDACKGDSGG PLVCKHSGRW QLVGITSWGE
610 620 630 638
GCARKDQPGV YTKVSEYMDW ILEKTQSSDV RALETSSA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-682 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLKB1
Peptide Modified Sequence
IYPGVDFGGEELN[+1.0]VTFVK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
308
Peptide - Site of Modification
13
Peptide Start
296
Peptide End
313
CPTAC ID
CPTAC-682
Peptide Molecular Mass
1,983.9829
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (1+) 9.6 3.3 2.7 15.6 3 2.3 18.3 4.5 3.5 15 15 15
y11 (1+) 5.1 2.8 2.8 13.3 3.3 2.7 14.2 4.3 3.9 15 15 15
y16 (2+) 6.2 2.6 2.8 14.6 2.6 2.9 15.9 3.7 4 15 15 15
sum 5.3 2 2.3 13.8 2.2 2.2 14.8 3 3.2 15 15 15

Additional Resources and Comments

Resources
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Assay Details for CPTAC-683 Collapse assay details

Data source: Panorama

Official Gene Symbol
KLKB1
Peptide Modified Sequence
IYSGILN[+1.0]LSDITK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
453
Peptide - Site of Modification
7
Peptide Start
447
Peptide End
459
CPTAC ID
CPTAC-683
Peptide Molecular Mass
1,436.7762
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 3.6 2.9 4.6 3.9 2.7 4.3 5.3 4 6.3 15 15 15
y8 (1+) 5.5 4.5 4.7 5.3 3.3 4.1 7.6 5.6 6.2 15 15 15
y7 (1+) 4.9 4.6 5.6 5.2 4.1 5.3 7.1 6.2 7.7 15 15 15
sum 3.1 3.7 4.5 2.8 3 4.2 4.2 4.8 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-5603 Collapse assay details

Data source: Panorama

Official Gene Symbol
Klkb1
Peptide Sequence
TGAISGHSLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
93
Peptide End
102
CPTAC ID
non-CPTAC-5603
Peptide Molecular Mass
969.5243
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 15 8.2 5.7 19 10.2 6.4 24.2 13.1 8.6 15 15 15
y7 (2+) 18.6 6.2 5.7 20.5 12 8.2 27.7 13.5 10 15 15 15
y8 (2+) 9.7 8.7 7.3 10.9 10.4 6.8 14.6 13.6 10 15 15 15
y9 (2+) 7.5 8.3 5.6 10.2 10.2 6.5 12.7 13.2 8.6 15 15 15
y6 (1+) 7.6 4.8 6.2 8.3 6.7 6.8 11.3 8.2 9.2 15 15 15
sum 6.7 4.2 3.2 8.5 7.3 4.8 10.8 8.4 5.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments