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Overview Data source: UniProt

Official Gene Symbol Other Aliases
FGG N/A
Sequence Length (AA) Molecular Weight (Da)
453 51512
Protein Name
Fibrinogen gamma chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSWSLHPRNL ILYFYALLFL SSTCVAYVAT RDNCCILDER FGSYCPTTCG
60 70 80 90 100
IADFLSTYQT KVDKDLQSLE DILHQVENKT SEVKQLIKAI QLTYNPDESS
110 120 130 140 150
KPNMIDAATL KSRKMLEEIM KYEASILTHD SSIRYLQEIY NSNNQKIVNL
160 170 180 190 200
KEKVAQLEAQ CQEPCKDTVQ IHDITGKDCQ DIANKGAKQS GLYFIKPLKA
210 220 230 240 250
NQQFLVYCEI DGSGNGWTVF QKRLDGSVDF KKNWIQYKEG FGHLSPTGTT
260 270 280 290 300
EFWLGNEKIH LISTQSAIPY ALRVELEDWN GRTSTADYAM FKVGPEADKY
310 320 330 340 350
RLTYAYFAGG DAGDAFDGFD FGDDPSDKFF TSHNGMQFST WDNDNDKFEG
360 370 380 390 400
NCAEQDGSGW WMNKCHAGHL NGVYYQGGTY SKASTPNGYD NGIIWATWKT
410 420 430 440 450
RWYSMKKTTM KIIPFNRLTI GEGQQHHLGG AKQVRPEHPA ETEYDSLYPE
453
DDL

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3323 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fgg
Peptide Sequence
YEALLLTHETSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
120
Peptide End
133
CPTAC ID
non-CPTAC-3323
Peptide Molecular Mass
1,544.8199
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 5.1 5.8 5 12.3 9.2 5.6 13.3 10.9 7.5 15 15 15
y12 (2+) 6.3 5.4 1.9 15.5 7.1 5 16.7 8.9 5.3 15 15 15
y11 (2+) 6.1 4.2 4 13.6 6.7 10.6 14.9 7.9 11.3 15 15 15
y4 (1+) 6.1 3.1 3.3 15.2 5.5 8.2 16.4 6.3 8.8 15 15 15
b2 (1+) 6.3 3.7 5.4 12.9 5.3 7.1 14.4 6.5 8.9 15 15 15
sum 3.8 3.7 2.9 12.9 5.8 6.9 13.4 6.9 7.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1121 Collapse assay details

Data source: Panorama

Official Gene Symbol
FGG
Peptide Sequence
YEASILTHDSSIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
122
Peptide End
134
CPTAC ID
non-CPTAC-1121
Peptide Molecular Mass
1,490.7365
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.1 7.1 7.8 12.7 7.6 7.8 13.3 10.4 11 15 15 15
y10 (2+) 10.1 5.2 7.1 12.2 6.9 8.1 15.8 8.6 10.8 15 15 15
y11 (2+) 5.9 4.6 5.3 11.4 5.7 6.6 12.8 7.3 8.5 15 15 15
y12 (2+) 7.6 5.4 4.6 11.1 6.2 4.9 13.5 8.2 6.7 15 15 15
y7 (1+) 6.3 3.9 8.1 15.7 9.6 8.7 16.9 10.4 11.9 15 15 15
sum 2.7 3.6 2.8 10.9 5.1 3.8 11.2 6.2 4.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3431 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fgg
Peptide Sequence
YLQEIYNSNNQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
134
Peptide End
145
CPTAC ID
non-CPTAC-3431
Peptide Molecular Mass
1,512.7209
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 7.5 3.4 5 19.4 3.6 8.5 20.8 5 9.9 15 14 15
y10 (2+) 5.4 6.4 4.4 13.8 6.3 8 14.8 9 9.1 15 14 15
y6 (1+) 4.5 4.3 4.2 15.2 5.8 4.4 15.9 7.2 6.1 15 14 15
y7 (1+) 5.2 4.9 2.7 13.9 7 6 14.8 8.5 6.6 15 14 15
y8 (1+) 5.7 5.4 3.4 9.9 4.7 7.3 11.4 7.2 8.1 15 14 15
sum 2 2.2 1.9 13.2 3.5 5.1 13.4 4.1 5.4 15 14 15

Additional Resources and Comments