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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
SERPINA5 PCI, PLANH3, PROCI
Sequence Length (AA) Molecular Weight (Da)
406 45675
Protein Name
Plasma serine protease inhibitor
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQLFLLLCLV LLSPQGASLH RHHPREMKKR VEDLHVGATV APSSRRDFTF
60 70 80 90 100
DLYRALASAA PSQSIFFSPV SISMSLAMLS LGAGSSTKMQ ILEGLGLNLQ
110 120 130 140 150
KSSEKELHRG FQQLLQELNQ PRDGFQLSLG NALFTDLVVD LQDTFVSAMK
160 170 180 190 200
TLYLADTFPT NFRDSAGAMK QINDYVAKQT KGKIVDLLKN LDSNAVVIMV
210 220 230 240 250
NYIFFKAKWE TSFNHKGTQE QDFYVTSETV VRVPMMSRED QYHYLLDRNL
260 270 280 290 300
SCRVVGVPYQ GNATALFILP SEGKMQQVEN GLSEKTLRKW LKMFKKRQLE
310 320 330 340 350
LYLPKFSIEG SYQLEKVLPS LGISNVFTSH ADLSGISNHS NIQVSEMVHK
360 370 380 390 400
AVVEVDESGT RAAAATGTIF TFRSARLNSQ RLVFNRPFLM FIVDNNILFL
406
GKVNRP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1149 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINA5
Peptide Sequence
AVVEVDESGTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
351
Peptide End
361
CPTAC ID
non-CPTAC-1149
Peptide Molecular Mass
1,160.5673
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 31.2 12.3 8.3 32.8 13.6 5.6 45.3 18.3 10 15 15 15
b3 (1+) 25.3 9.3 10.7 23.2 8.3 9.4 34.3 12.5 14.2 15 15 15
y9 (2+) 15.4 13.6 6.7 17.6 15.2 6.9 23.4 20.4 9.6 15 15 15
y6 (1+) 20.8 8.6 9.4 20.1 10.8 9.1 28.9 13.8 13.1 15 15 15
y7 (1+) 15.6 10.1 5.3 16.9 12.6 6.8 23 16.1 8.6 15 15 15
sum 5.7 7.7 4.4 8.3 10 4.6 10.1 12.6 6.4 15 15 15


Additional Resources and Comments