SERPINA5, CPTAC-1149 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SERPINA5	PCI, PLANH3, PROCI

Sequence Length (AA)	Molecular Weight (Da)
406	45675

Protein Name
Plasma serine protease inhibitor

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQLFLLLCLV	LLSPQGASLH	RHHPREMKKR	VEDLHVGATV	APSSRRDFTF
60	70	80	90	100
DLYRALASAA	PSQSIFFSPV	SISMSLAMLS	LGAGSSTKMQ	ILEGLGLNLQ
110	120	130	140	150
KSSEKELHRG	FQQLLQELNQ	PRDGFQLSLG	NALFTDLVVD	LQDTFVSAMK
160	170	180	190	200
TLYLADTFPT	NFRDSAGAMK	QINDYVAKQT	KGKIVDLLKN	LDSNAVVIMV
210	220	230	240	250
NYIFFKAKWE	TSFNHKGTQE	QDFYVTSETV	VRVPMMSRED	QYHYLLDRNL
260	270	280	290	300
SCRVVGVPYQ	GNATALFILP	SEGKMQQVEN	GLSEKTLRKW	LKMFKKRQLE
310	320	330	340	350
LYLPKFSIEG	SYQLEKVLPS	LGISNVFTSH	ADLSGISNHS	NIQVSEMVHK
360	370	380	390	400
AVVEVDESGT	RAAAATGTIF	TFRSARLNSQ	RLVFNRPFLM	FIVDNNILFL
406
GKVNRP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-1149 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINA5
Peptide Sequence: AVVEVDESGTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 351
Peptide End: 361

CPTAC ID: non-CPTAC-1149
Peptide Molecular Mass: 1,160.5673
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (1+)	31.2	12.3	8.3	32.8	13.6	5.6	45.3	18.3	10	15	15	15
b3 (1+)	25.3	9.3	10.7	23.2	8.3	9.4	34.3	12.5	14.2	15	15	15
y9 (2+)	15.4	13.6	6.7	17.6	15.2	6.9	23.4	20.4	9.6	15	15	15
y6 (1+)	20.8	8.6	9.4	20.1	10.8	9.1	28.9	13.8	13.1	15	15	15
y7 (1+)	15.6	10.1	5.3	16.9	12.6	6.8	23	16.1	8.6	15	15	15
sum	5.7	7.7	4.4	8.3	10	4.6	10.1	12.6	6.4	15	15	15

SERPINA5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-1149 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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SERPINA5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-1149 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments