SERPINE1, CPTAC-1150 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SERPINE1	PAI1, PLANH1

Sequence Length (AA)	Molecular Weight (Da)
402	45060

Protein Name
Plasminogen activator inhibitor 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MQMSPALTCL	VLGLALVFGE	GSAVHHPPSY	VAHLASDFGV	RVFQQVAQAS
60	70	80	90	100
KDRNVVFSPY	GVASVLAMLQ	LTTGGETQQQ	IQAAMGFKID	DKGMAPALRH
110	120	130	140	150
LYKELMGPWN	KDEISTTDAI	FVQRDLKLVQ	GFMPHFFRLF	RSTVKQVDFS
160	170	180	190	200
EVERARFIIN	DWVKTHTKGM	ISNLLGKGAV	DQLTRLVLVN	ALYFNGQWKT
210	220	230	240	250
PFPDSSTHRR	LFHKSDGSTV	SVPMMAQTNK	FNYTEFTTPD	GHYYDILELP
260	270	280	290	300
YHGDTLSMFI	AAPYEKEVPL	SALTNILSAQ	LISHWKGNMT	RLPRLLVLPK
310	320	330	340	350
FSLETEVDLR	KPLENLGMTD	MFRQFQADFT	SLSDQEPLHV	AQALQKVKIE
360	370	380	390	400
VNESGTVASS	STAVIVSARM	APEEIIMDRP	FLFVVRHNPT	GTVLFMGQVM
402
EP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-4049 Collapse assay details

Data source: Panorama

Official Gene Symbol: Serpine1
Peptide Sequence: FSLETEVDLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 301
Peptide End: 310

CPTAC ID: non-CPTAC-4049
Peptide Molecular Mass: 1,207.6085
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	11.3	9.4	5.3	10.4	10	7	15.4	13.7	8.8	15	15	15
y7 (1+)	11.9	9.4	7.3	12.3	10	8.2	17.1	13.7	11	15	15	15
y6 (1+)	16.5	20.4	19	21	23.2	17.6	26.7	30.9	25.9	15	15	15
y2 (1+)	9.9	6.7	6.1	10	7.6	5.3	14.1	10.1	8.1	15	15	15
y3 (2+)	104.5	43.4	75.4	116.9	93.9	75.2	156.8	103.4	106.5	15	15	15
sum	9.1	8.7	7.4	10.4	8.8	7	13.8	12.4	10.2	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1150 Collapse assay details

Data source: Panorama

Official Gene Symbol: SERPINE1
Peptide Sequence: VFQQVAQASK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 42
Peptide End: 51

CPTAC ID: non-CPTAC-1150
Peptide Molecular Mass: 1,104.5928
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers