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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpine1 Mr1, Pai1, Planh1
Sequence Length (AA) Molecular Weight (Da)
402 45170
Protein Name
Plasminogen activator inhibitor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQMSSALACL ILGLVLVSGK GFTLPLRESH TAHQATDFGV KVFQQVVQAS
60 70 80 90 100
KDRNVVFSPY GVSSVLAMLQ MTTAGKTRRQ IQDAMGFKVN EKGTAHALRQ
110 120 130 140 150
LSKELMGPWN KNEISTADAI FVQRDLELVQ GFMPHFFKLF QTMVKQVDFS
160 170 180 190 200
EVERARFIIN DWVERHTKGM INDLLAKGAV DELTRLVLVN ALYFSGQWKT
210 220 230 240 250
PFLEASTHQR LFHKSDGSTV SVPMMAQSNK FNYTEFTTPD GLEYDVVELP
260 270 280 290 300
YQRDTLSMFI AAPFEKDVHL SALTNILDAE LIRQWKGNMT RLPRLLILPK
310 320 330 340 350
FSLETEVDLR GPLEKLGMPD MFSATLADFT SLSDQEQLSV AQALQKVRIE
360 370 380 390 400
VNESGTVASS STAFVISARM APTEMVIDRS FLFVVRHNPT ETILFMGQVM
402
EP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4049 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpine1
Peptide Sequence
FSLETEVDLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
301
Peptide End
310
CPTAC ID
non-CPTAC-4049
Peptide Molecular Mass
1,207.6085
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 11.3 9.4 5.3 10.4 10 7 15.4 13.7 8.8 15 15 15
y7 (1+) 11.9 9.4 7.3 12.3 10 8.2 17.1 13.7 11 15 15 15
y6 (1+) 16.5 20.4 19 21 23.2 17.6 26.7 30.9 25.9 15 15 15
y2 (1+) 9.9 6.7 6.1 10 7.6 5.3 14.1 10.1 8.1 15 15 15
y3 (2+) 104.5 43.4 75.4 116.9 93.9 75.2 156.8 103.4 106.5 15 15 15
sum 9.1 8.7 7.4 10.4 8.8 7 13.8 12.4 10.2 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-1150 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINE1
Peptide Sequence
VFQQVAQASK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
42
Peptide End
51
CPTAC ID
non-CPTAC-1150
Peptide Molecular Mass
1,104.5928
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 12.1 5.1 8.5 15.6 8.3 11 19.7 9.7 13.9 14 15 15
y8 (2+) 17.5 11.7 9.6 17.1 10.6 12.7 24.5 15.8 15.9 15 15 15
y5 (1+) 9.7 10.6 7.4 8.9 13.6 8.2 13.2 17.2 11 15 15 15
y7 (1+) 28.7 10.3 7.5 23.1 12.4 8.8 36.8 16.1 11.6 15 15 15
y8 (1+) 16.9 11 7 16.9 10.5 7.4 23.9 15.2 10.2 15 15 15
sum 8.6 5.3 4.8 7.6 5.9 4.5 11.5 7.9 6.6 15 15 15


Additional Resources and Comments