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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Pcdh18 Kiaa1562
Sequence Length (AA) Molecular Weight (Da)
1134 125420
Protein Name
Protocadherin 18
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHQMNTKMHF RFALALLMAF FSHDVLAKNL KYRIYEEQRV GSVIARLSED
60 70 80 90 100
VADVLLKLPN PSAVRFRAMP RGNSPLLVVN ENTGEISIGA KIDREQLCQK
110 120 130 140 150
NLNCSIEFDV LTLPTEHLQL FHIEVDVLDI NDNSPQFSRP VIPIEISESA
160 170 180 190 200
AVGTRIPLDS AFDPDVGENS LHTYSLSAND YFNIEVRTRT DGAKYAELIV
210 220 230 240 250
VKELDRELKA SYELQLTASD MGVPQRSGSS ILKISISDSN DNSPAFEQPS
260 270 280 290 300
YTIQLLENSP VGTLLLDLNA TDPDEGANGR IVYSFSSHVS PKIIETFKID
310 320 330 340 350
SEKGHLTLFK PVDYEITKSY EIDVQAQDLG PNSIPAHCKI IIKVVDVNDN
360 370 380 390 400
KPEISINLMS PGKEEVSYVF EGDPIDTFVA IVRVQDKDSG LNGEIICKLH
410 420 430 440 450
GHGHFKLQKT YENNYLILTN ATLDREKRSE YSLTVIAEDK GTPSLSSVRH
460 470 480 490 500
FTVQINDIND NPPRFQRSRY EFVISENNSP GAYITTVTAT DPDLGENGHV
510 520 530 540 550
TYTILESFVL GSSITTYVTI DPSNGAIYAL RIFDHEEVSQ ITFVVEARDG
560 570 580 590 600
GSQKQLSSNT TVVLTIIDEN DNVPVVIGPA MHNNTAEISI PKGAESGFHV
610 620 630 640 650
TRIRVVDRDS GANAEFSCSI VSGNEENIFI MDPRSCDIHT NVSMESIPSA
660 670 680 690 700
EWALSVIIQD KGSPPLHTKV LLRCMVFDYA ESVTSTAMTS VSRASLDVSM
710 720 730 740 750
IIIISLGAIC AVLLVIMVLF ATRCNREKKD TRSYNCRVAE STYQHHPKRP
760 770 780 790 800
SRQIHKGDIT LVPTINGTLP IRSHHRSSPS SSPTLERGQM GSRQSHNSHQ
810 820 830 840 850
SLNSLVTISS NHVPENFSLE LTHATPAVEV SQLLSMLHQG QYQPRPSFRG
860 870 880 890 900
NKYSRSYRYA LQDMDKFSLK DSGRGDSEAG DSDYDLGRDS PIDRLLGEGF
910 920 930 940 950
SDLFLTDGRI PAAMRLCTEE CRVLGHSDQC WMPPLPSPSS DYRSNMFIPG
960 970 980 990 1000
EEFPAQPQQQ HSHQGLDDDS QPAENGEKKK SFSTFGKDSP SDEDSGDSST
1010 1020 1030 1040 1050
SSLLSEMSSV FQRLLPASLD TFSECNEGDR SNSLERRKGP AQGKTGGYPQ
1060 1070 1080 1090 1100
GVAAWAASTH FQNPTSSSGT PLGTHSSVQP SSKWLPAMEE IPENYEEDDF
1110 1120 1130 1134
DNVLNHLSDG KHELMDASEL VAEINKLLQD VRQS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4050 Collapse assay details

Data source: Panorama

Official Gene Symbol
Pcdh18
Peptide Sequence
IVYSFSSHVSPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
281
Peptide End
292
CPTAC ID
non-CPTAC-4050
Peptide Molecular Mass
1,349.6980
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.6 2.8 4.4 8.7 4.8 3.9 10.9 5.6 5.9 15 15 15
y8 (2+) 22.4 4.4 7.7 34.9 14.5 5.8 41.5 15.2 9.6 15 15 15
y9 (2+) 17.2 6.3 2.8 22.4 7.4 3.5 28.2 9.7 4.5 15 15 15
y10 (2+) 3.1 2.6 3 7.4 3.8 4.2 8 4.6 5.2 15 15 15
y11 (2+) 13.4 2.9 5.3 10.2 3.2 5.8 16.8 4.3 7.9 15 15 15
sum 3.7 2.1 2.3 8.5 3.4 3.3 9.3 4 4 15 15 15


Additional Resources and Comments