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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nedd8 Nedd-8
Sequence Length (AA) Molecular Weight (Da)
81 8972
Protein Name
NEDD8
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM
60 70 80 81
NDEKTAADYK ILGGSVLHLV LALRGGGGLG Q

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4051 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nedd8
Peptide Sequence
EIEIDIEPTDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
12
Peptide End
22
CPTAC ID
non-CPTAC-4051
Peptide Molecular Mass
1,300.6398
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 17.4 3.6 2.5 21.4 5.3 6.4 27.6 6.4 6.9 15 15 15
y7 (1+) 5.5 3.7 3 11.9 3.5 4.9 13.1 5.1 5.7 15 15 15
y5 (1+) 8.5 3.2 2.6 12.8 4.7 3.8 15.4 5.7 4.6 15 15 15
y4 (1+) 9.9 3.7 3.2 10.2 5 5.8 14.2 6.2 6.6 15 15 15
b2 (1+) 21.7 2.8 4.1 22.9 5.3 4.9 31.5 6 6.4 15 15 15
sum 7.2 2.1 1.4 10.2 4 4.4 12.5 4.5 4.6 15 15 15


Additional Resources and Comments