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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nqo1 Dia4, Nmo1, Nmor1
Sequence Length (AA) Molecular Weight (Da)
274 30960
Protein Name
NAD(P)H dehydrogenase [quinone] 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAARRALIVL AHSEKTSFNY AMKEAAVEAL KKRGWEVLES DLYAMNFNPI
60 70 80 90 100
ISRNDITGEL KDSKNFQYPS ESSLAYKEGR LSPDIVAEHK KLEAADLVIF
110 120 130 140 150
QFPLQWFGVP AILKGWFERV LVAGFAYTYA AMYDNGPFQN KKTLLSITTG
160 170 180 190 200
GSGSMYSLQG VHGDMNVILW PIQSGILRFC GFQVLEPQLV YSIGHTPPDA
210 220 230 240 250
RMQILEGWKK RLETVWEETP LYFAPSSLFD LNFQAGFLMK KEVQEEQKKN
260 270 274
KFGLSVGHHL GKSIPADNQI KARK

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4052 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nqo1
Peptide Sequence
FGLSVGHHLGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
252
Peptide End
262
CPTAC ID
non-CPTAC-4052
Peptide Molecular Mass
1,150.6247
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 90.2 84.3 56.8 118.2 66.5 103.3 148.7 107.4 117.9 15 15 15
y10 (2+) 22.3 8.7 10.8 25.9 14 9.7 34.2 16.5 14.5 15 15 15
y9 (2+) 12.5 13.5 9.3 22.9 16 10.1 26.1 20.9 13.7 15 15 15
y8 (2+) 8.7 3.7 5.7 18.7 7.6 8 20.6 8.5 9.8 15 15 15
b2 (1+) 9.3 5.5 7.1 9.2 6.1 7.7 13.1 8.2 10.5 15 15 15
sum 8.1 3.6 4.7 13.9 6 5.4 16.1 7 7.2 15 15 15


Additional Resources and Comments