RBP4, CPTAC-1156 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RBP4	N/A

Sequence Length (AA)	Molecular Weight (Da)
201	23010

Protein Name
Retinol-binding protein 4

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKWVWALLLL	AALGSGRAER	DCRVSSFRVK	ENFDKARFSG	TWYAMAKKDP
60	70	80	90	100
EGLFLQDNIV	AEFSVDETGQ	MSATAKGRVR	LLNNWDVCAD	MVGTFTDTED
110	120	130	140	150
PAKFKMKYWG	VASFLQKGND	DHWIVDTDYD	TYAVQYSCRL	LNLDGTCADS
160	170	180	190	200
YSFVFSRDPN	GLPPEAQKIV	RQRQEELCLA	RQYRLIVHNG	YCDGRSERNL
201
L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3736 Collapse assay details

Data source: Panorama

Official Gene Symbol: Rbp4
Peptide Sequence: DPNGLSPETR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 158
Peptide End: 167

CPTAC ID: non-CPTAC-3736
Peptide Molecular Mass: 1,084.5149
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	9.8	8.3	7.6	11.5	15	14.4	15.1	17.1	16.3	15	15	15
b2 (1+)	17.1	5.4	7.8	20.6	5.6	8.5	26.8	7.8	11.5	15	15	15
y2 (1+)	17.5	6.4	6.8	21	11.8	14.1	27.3	13.4	15.7	15	15	15
y4 (1+)	11.1	8.3	5	17.4	8.6	6.7	20.6	12	8.4	15	15	15
y5 (1+)	12	5.5	6.4	11.7	4.7	8.1	16.8	7.2	10.3	15	15	15
sum	6.7	4.4	2.5	9	6.1	7.4	11.2	7.5	7.8	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-1156 Collapse assay details

Data source: Panorama

Official Gene Symbol: RBP4
Peptide Sequence: YWGVASFLQK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 108
Peptide End: 117

CPTAC ID: non-CPTAC-1156
Peptide Molecular Mass: 1,197.6182
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers