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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AZGP1 ZAG, ZNGP1
Sequence Length (AA) Molecular Weight (Da)
298 34259
Protein Name
Zinc-alpha-2-glycoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVRMVPVLLS LLLLLGPAVP QENQDGRYSL TYIYTGLSKH VEDVPAFQAL
60 70 80 90 100
GSLNDLQFFR YNSKDRKSQP MGLWRQVEGM EDWKQDSQLQ KAREDIFMET
110 120 130 140 150
LKDIVEYYND SNGSHVLQGR FGCEIENNRS SGAFWKYYYD GKDYIEFNKE
160 170 180 190 200
IPAWVPFDPA AQITKQKWEA EPVYVQRAKA YLEEECPATL RKYLKYSKNI
210 220 230 240 250
LDRQDPPSVV VTSHQAPGEK KKLKCLAYDF YPGKIDVHWT RAGEVQEPEL
260 270 280 290 298
RGDVLHNGNG TYQSWVVVAV PPQDTAPYSC HVQHSSLAQP LVVPWEAS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2709 Collapse assay details

Data source: Panorama

Official Gene Symbol
AZGP1
Peptide Sequence
EIPAWVPFDPAAQITK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
150
Peptide End
165
CPTAC ID
non-CPTAC-2709
Peptide Molecular Mass
1,781.9352
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.9 4.3 7 10.9 9.8 9.7 12.9 10.7 12 15 15 15
y7 (1+) 7.4 3.4 7.1 11.7 9.5 9.5 13.8 10.1 11.9 15 15 15
y14 (2+) 7.2 3.9 8.7 13.1 8.4 11 14.9 9.3 14 15 15 15
y8 (2+) 61.6 49.8 17.8 78 57.1 61.1 99.4 75.8 63.6 15 15 15
b6 (1+) 6.1 5 9.3 11.4 8.1 11.1 12.9 9.5 14.5 15 15 15
sum 5 3.6 7.7 11.1 8.6 10.1 12.2 9.3 12.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3392 Collapse assay details

Data source: Panorama

Official Gene Symbol
Azgp1
Peptide Sequence
LAFEPER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
241
Peptide End
247
CPTAC ID
non-CPTAC-3392
Peptide Molecular Mass
860.4392
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (2+) 6.8 5.5 7.8 13.9 8.2 9.5 15.5 9.9 12.3 15 15 15
y3 (1+) 7 3 11.8 14.7 8 11 16.3 8.5 16.1 15 15 15
y4 (1+) 3.8 4.7 5.4 11.8 6.5 6.1 12.4 8 8.1 15 15 15
y5 (1+) 4.2 5.8 8.9 15.2 9.6 8.8 15.8 11.2 12.5 15 15 15
y6 (1+) 4.2 4 8.6 14 6.8 11.5 14.6 7.9 14.4 15 15 15
sum 2.6 2.1 4.3 13.5 7 4.4 13.7 7.3 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3391 Collapse assay details

Data source: Panorama

Official Gene Symbol
Azgp1
Peptide Sequence
VIPGGNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
210
Peptide End
216
CPTAC ID
non-CPTAC-3391
Peptide Molecular Mass
711.4028
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (2+) 96.7 79.8 75.3 84.9 116.3 82.7 128.7 141 111.8 15 15 15
y5 (2+) 6.3 6.9 4 12.2 8.8 10.7 13.7 11.2 11.4 15 15 15
y3 (1+) 22.4 9.1 9 28.5 6.4 13.3 36.2 11.1 16.1 15 15 15
y4 (1+) 6.8 6.7 7.1 16.8 9.9 11.1 18.1 12 13.2 15 15 15
y5 (1+) 4.5 4.9 7.1 14.7 7.1 9.6 15.4 8.6 11.9 15 15 15
sum 3.7 4.1 3.3 12.2 7 7.6 12.7 8.1 8.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1167 Collapse assay details

Data source: Panorama

Official Gene Symbol
AZGP1
Peptide Sequence
YSLTYIYTGLSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
28
Peptide End
39
CPTAC ID
non-CPTAC-1167
Peptide Molecular Mass
1,407.7286
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 7.7 5.6 7.7 7.6 10.6 8.8 10.8 12 11.7 15 15 15
y6 (1+) 12.6 7.9 5.4 11.1 11 9.4 16.8 13.5 10.8 15 15 15
y7 (1+) 9.1 5.4 7.9 9.2 9.2 7.8 12.9 10.7 11.1 15 15 15
y8 (1+) 9.2 7.2 6.8 10.4 14 8.9 13.9 15.7 11.2 15 15 15
y9 (1+) 13 8 4.4 11 11.1 4.6 17 13.7 6.4 15 15 15
sum 7.2 4.6 4.5 5.3 9.6 5.6 8.9 10.6 7.2 15 15 15

Additional Resources and Comments