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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MMP2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MMP2 CLG4A
Sequence Length (AA) Molecular Weight (Da)
660 73882
Protein Name
72 kDa type IV collagenase
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEALMARGAL TGPLRALCLL GCLLSHAAAA PSPIIKFPGD VAPKTDKELA
60 70 80 90 100
VQYLNTFYGC PKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP
110 120 130 140 150
RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV
160 170 180 190 200
WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG
210 220 230 240 250
VGGDSHFDDD ELWTLGEGQV VRVKYGNADG EYCKFPFLFN GKEYNSCTDT
260 270 280 290 300
GRSDGFLWCS TTYNFEKDGK YGFCPHEALF TMGGNAEGQP CKFPFRFQGT
310 320 330 340 350
SYDSCTTEGR TDGYRWCGTT EDYDRDKKYG FCPETAMSTV GGNSEGAPCV
360 370 380 390 400
FPFTFLGNKY ESCTSAGRSD GKMWCATTAN YDDDRKWGFC PDQGYSLFLV
410 420 430 440 450
AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSQDDIKG IQELYGASPD
460 470 480 490 500
IDLGTGPTPT LGPVTPEICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR
510 520 530 540 550
DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER
560 570 580 590 600
GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP
610 620 630 640 650
GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF
660
GSIKSDWLGC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2615 Collapse assay details

Data source: Panorama

Official Gene Symbol
MMP2
Peptide Sequence
AFQVWSDVTPLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
147
Peptide End
158
CPTAC ID
non-CPTAC-2615
Peptide Molecular Mass
1,417.7354
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 7.2 7.1 10.9 13.7 8 14.2 15.5 10.7 17.9 15 15 15
y7 (1+) 7 7.1 10.7 11.2 9 12.1 13.2 11.5 16.2 15 15 15
b3 (1+) 8.2 6.5 9.5 15.8 8.9 11 17.8 11 14.5 15 15 15
y5 (1+) 12.9 8.4 14.5 14.3 9.7 16.7 19.3 12.8 22.1 15 15 15
y4 (1+) 9.5 4.9 8.4 14.5 6.7 14.8 17.3 8.3 17 15 15 15
sum 3.5 5.5 8.5 11.2 7.5 12 11.7 9.3 14.7 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-3729 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mmp2
Peptide Sequence
IDAVYEAPQEEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
522
Peptide End
533
CPTAC ID
non-CPTAC-3729
Peptide Molecular Mass
1,390.6616
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 11.1 6.9 5.7 14.2 8.7 8.4 18 11.1 10.2 15 15 15
b3 (1+) 11 5 5.4 7.8 5.4 7.6 13.5 7.4 9.3 15 15 15
b2 (1+) 10.2 6.4 5.6 9.4 7.6 7.1 13.9 9.9 9 15 15 15
y5 (1+) 10.9 6.2 6 10.8 7.6 7.6 15.3 9.8 9.7 15 15 15
y8 (1+) 6.4 3.5 4.7 6 7.7 7.3 8.8 8.5 8.7 15 15 15
sum 4.1 2.1 2.4 3.9 4.7 5.7 5.7 5.1 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments