HABP2, CPTAC-2675 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
HABP2	HGFAL, PHBP

Sequence Length (AA)	Molecular Weight (Da)
560	62672

Protein Name
Hyaluronan-binding protein 2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MFARMSDLHV	LLLMALVGKT	ACGFSLMSLL	ESLDPDWTPD	QYDYSYEDYN
60	70	80	90	100
QEENTSSTLT	HAENPDWYYT	EDQADPCQPN	PCEHGGDCLV	HGSTFTCSCL
110	120	130	140	150
APFSGNKCQK	VQNTCKDNPC	GRGQCLITQS	PPYYRCVCKH	PYTGPSCSQV
160	170	180	190	200
VPVCRPNPCQ	NGATCSRHKR	RSKFTCACPD	QFKGKFCEIG	SDDCYVGDGY
210	220	230	240	250
SYRGKMNRTV	NQHACLYWNS	HLLLQENYNM	FMEDAETHGI	GEHNFCRNPD
260	270	280	290	300
ADEKPWCFIK	VTNDKVKWEY	CDVSACSAQD	VAYPEESPTE	PSTKLPGFDS
310	320	330	340	350
CGKTEIAERK	IKRIYGGFKS	TAGKHPWQAS	LQSSLPLTIS	MPQGHFCGGA
360	370	380	390	400
LIHPCWVLTA	AHCTDIKTRH	LKVVLGDQDL	KKEEFHEQSF	RVEKIFKYSH
410	420	430	440	450
YNERDEIPHN	DIALLKLKPV	DGHCALESKY	VKTVCLPDGS	FPSGSECHIS
460	470	480	490	500
GWGVTETGKG	SRQLLDAKVK	LIANTLCNSR	QLYDHMIDDS	MICAGNLQKP
510	520	530	540	550
GQDTCQGDSG	GPLTCEKDGT	YYVYGIVSWG	LECGKRPGVY	TQVTKFLNWI
560
KATIKSESGF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3295 Collapse assay details

Data source: Panorama

Official Gene Symbol: Habp2
Peptide Sequence: DEIPHNDIALLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 403
Peptide End: 414

CPTAC ID: non-CPTAC-3295
Peptide Molecular Mass: 1,376.7300
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

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Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	5.7	3	4.5	14.9	10.1	5.8	16	10.5	7.3	15	15	15
y8 (2+)	7.3	3.7	2.9	13.7	8.5	2.8	15.5	9.3	4	15	15	15
y2 (1+)	8.6	6.3	2.8	13.8	9.4	7.4	16.3	11.3	7.9	15	15	15
y10 (2+)	5.1	4.8	4.2	12.3	7.8	9.9	13.3	9.2	10.8	15	15	15
y9 (2+)	4.2	2.9	5	14.1	8.1	5.5	14.7	8.6	7.4	15	15	15
sum	1.6	2.6	2.2	12.9	7.7	5	13	8.1	5.5	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2675 Collapse assay details

Data source: Panorama

Official Gene Symbol: HABP2
Peptide Sequence: VVLGDQDLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 373
Peptide End: 381

CPTAC ID: non-CPTAC-2675
Peptide Molecular Mass: 985.5444
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers