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Overview Data source: UniProt

Official Gene Symbol Other Aliases
SPARC ON
Sequence Length (AA) Molecular Weight (Da)
303 34632
Protein Name
SPARC
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRAWIFFLLC LAGRALAAPQ QEALPDETEV VEETVAEVTE VSVGANPVQV
60 70 80 90 100
EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS
110 120 130 140 150
CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK
160 170 180 190 200
YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH
210 220 230 240 250
ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT
260 270 280 290 300
ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKQKDIDKD
303
LVI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2699 Collapse assay details

Data source: Panorama

Official Gene Symbol
SPARC
Peptide Sequence
LEAGDHPVELLAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
205
Peptide End
217
CPTAC ID
non-CPTAC-2699
Peptide Molecular Mass
1,418.7518
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12 (2+) 9.5 8.4 11.1 13.1 11.4 11.8 16.2 14.2 16.2 15 15 15
y7 (1+) 8.3 12.6 9.6 11.8 12 14 14.4 17.4 17 15 15 15
y8 (2+) 20.6 8.9 14.4 19 7.6 13.9 28 11.7 20 15 15 15
y10 (2+) 21.6 11.5 9.5 28.7 19.4 16.5 35.9 22.6 19 15 15 15
y11 (2+) 7.1 5.3 9.6 12.4 8.5 10 14.3 10 13.9 15 15 15
sum 5.1 3.6 7.1 10.7 8.3 9.7 11.9 9 12 15 15 15

Additional Resources and Comments