SPARC, CPTAC-2699 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
SPARC	ON

Sequence Length (AA)	Molecular Weight (Da)
303	34632

Protein Name
SPARC

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MRAWIFFLLC	LAGRALAAPQ	QEALPDETEV	VEETVAEVTE	VSVGANPVQV
60	70	80	90	100
EVGEFDDGAE	ETEEEVVAEN	PCQNHHCKHG	KVCELDENNT	PMCVCQDPTS
110	120	130	140	150
CPAPIGEFEK	VCSNDNKTFD	SSCHFFATKC	TLEGTKKGHK	LHLDYIGPCK
160	170	180	190	200
YIPPCLDSEL	TEFPLRMRDW	LKNVLVTLYE	RDEDNNLLTE	KQKLRVKKIH
210	220	230	240	250
ENEKRLEAGD	HPVELLARDF	EKNYNMYIFP	VHWQFGQLDQ	HPIDGYLSHT
260	270	280	290	300
ELAPLRAPLI	PMEHCTTRFF	ETCDLDNDKY	IALDEWAGCF	GIKQKDIDKD
303
LVI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2699 Collapse assay details

Data source: Panorama

Official Gene Symbol: SPARC
Peptide Sequence: LEAGDHPVELLAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 205
Peptide End: 217

CPTAC ID: non-CPTAC-2699
Peptide Molecular Mass: 1,418.7518
Species: Homo sapiens (Human)
Assay Type: Direct MRM
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y12 (2+)	9.5	8.4	11.1	13.1	11.4	11.8	16.2	14.2	16.2	15	15	15
y7 (1+)	8.3	12.6	9.6	11.8	12	14	14.4	17.4	17	15	15	15
y8 (2+)	20.6	8.9	14.4	19	7.6	13.9	28	11.7	20	15	15	15
y10 (2+)	21.6	11.5	9.5	28.7	19.4	16.5	35.9	22.6	19	15	15	15
y11 (2+)	7.1	5.3	9.6	12.4	8.5	10	14.3	10	13.9	15	15	15
sum	5.1	3.6	7.1	10.7	8.3	9.7	11.9	9	12	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3394 Collapse assay details

Data source: Panorama

Official Gene Symbol: Sparc
Peptide Sequence: LEAGDHPVELLAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 205
Peptide End: 217

CPTAC ID: non-CPTAC-3394
Peptide Molecular Mass: 1,418.7518
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.