CDK13, CPTAC-2883 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK13	CDC2L, CDC2L5, CHED, KIAA1791

Sequence Length (AA)	Molecular Weight (Da)
1512	164923

Protein Name
Cyclin-dependent kinase 13

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPSSSDTALG	GGGGLSWAEK	KLEERRKRRR	FLSPQQPPLL	LPLLQPQLLQ
60	70	80	90	100
PPPPPPPLLF	LAAPGTAAAA	AAAAAASSSC	FSPGPPLEVK	RLARGKRRAG
110	120	130	140	150
GRQKRRRGPR	AGQEAEKRRV	FSLPQPQQDG	GGGASSGGGV	TPLVEYEDVS
160	170	180	190	200
SQSEQGLLLG	GASAATAATA	AGGTGGSGGS	PASSSGTQRR	GEGSERRPRR
210	220	230	240	250
DRRSSSGRSK	ERHREHRRRD	GQRGGSEASK	SRSRHSHSGE	ERAEVAKSGS
260	270	280	290	300
SSSSGGRRKS	ASATSSSSSS	RKDRDSKAHR	SRTKSSKEPP	SAYKEPPKAY
310	320	330	340	350
REDKTEPKAY	RRRRSLSPLG	GRDDSPVSHR	ASQSLRSRKS	PSPAGGGSSP
360	370	380	390	400
YSRRLPRSPS	PYSRRRSPSY	SRHSSYERGG	DVSPSPYSSS	SWRRSRSPYS
410	420	430	440	450
PVLRRSGKSR	SRSPYSSRHS	RSRSRHRLSR	SRSRHSSISP	STLTLKSSLA
460	470	480	490	500
AELNKNKKAR	AAEAARAAEA	AKAAEATKAA	EAAAKAAKAS	NTSTPTKGNT
510	520	530	540	550
ETSASASQTN	HVKDVKKIKI	EHAPSPSSGG	TLKNDKAKTK	PPLQVTKVEN
560	570	580	590	600
NLIVDKATKK	AVIVGKESKS	AATKEESVSL	KEKTKPLTPS	IGAKEKEQHV
610	620	630	640	650
ALVTSTLPPL	PLPPMLPEDK	EADSLRGNIS	VKAVKKEVEK	KLRCLLADLP
660	670	680	690	700
LPPELPGGDD	LSKSPEEKKT	ATQLHSKRRP	KICGPRYGET	KEKDIDWGKR
710	720	730	740	750
CVDKFDIIGI	IGEGTYGQVY	KARDKDTGEM	VALKKVRLDN	EKEGFPITAI
760	770	780	790	800
REIKILRQLT	HQSIINMKEI	VTDKEDALDF	KKDKGAFYLV	FEYMDHDLMG
810	820	830	840	850
LLESGLVHFN	ENHIKSFMRQ	LMEGLDYCHK	KNFLHRDIKC	SNILLNNRGQ
860	870	880	890	900
IKLADFGLAR	LYSSEESRPY	TNKVITLWYR	PPELLLGEER	YTPAIDVWSC
910	920	930	940	950
GCILGELFTK	KPIFQANQEL	AQLELISRIC	GSPCPAVWPD	VIKLPYFNTM
960	970	980	990	1000
KPKKQYRRKL	REEFVFIPAA	ALDLFDYMLA	LDPSKRCTAE	QALQCEFLRD
1010	1020	1030	1040	1050
VEPSKMPPPD	LPLWQDCHEL	WSKKRRRQKQ	MGMTDDVSTI	KAPRKDLSLG
1060	1070	1080	1090	1100
LDDSRTNTPQ	GVLPSSQLKS	QGSSNVAPVK	TGPGQHLNHS	ELAILLNLLQ
1110	1120	1130	1140	1150
SKTSVNMADF	VQVLNIKVNS	ETQQQLNKIN	LPAGILATGE	KQTDPSTPQQ
1160	1170	1180	1190	1200
ESSKPLGGIQ	PSSQTIQPKV	ETDAAQAAVQ	SAFAVLLTQL	IKAQQSKQKD
1210	1220	1230	1240	1250
VLLEERENGS	GHEASLQLRP	PPEPSTPVSG	QDDLIQHQDM	RILELTPEPD
1260	1270	1280	1290	1300
RPRILPPDQR	PPEPPEPPPV	TEEDLDYRTE	NQHVPTTSSS	LTDPHAGVKA
1310	1320	1330	1340	1350
ALLQLLAQHQ	PQDDPKREGG	IDYQAGDTYV	STSDYKDNFG	SSSFSSAPYV
1360	1370	1380	1390	1400
SNDGLGSSSA	PPLERRSFIG	NSDIQSLDNY	STASSHSGGP	PQPSAFSESF
1410	1420	1430	1440	1450
PSSVAGYGDI	YLNAGPMLFS	GDKDHRFEYS	HGPIAVLANS	SDPSTGPEST
1460	1470	1480	1490	1500
HPLPAKMHNY	NYGGNLQENP	SGPSLMHGQT	WTSPAQGPGY	SQGYRGHIST
1510	1512
STGRGRGRGL	PY

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

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Assay Details for non-CPTAC-2883 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK13
Peptide Modified Sequence: IC[+57.0]GSPC[+57.0]PAVWPDVIK
Modification Type: Carbamidomethyl (C), Carbamidomethyl (C)
Protein - Site of Modification: 931, 935
Peptide - Site of Modification: 2, 6
Peptide Start: 929
Peptide End: 943

CPTAC ID: non-CPTAC-2883
Peptide Molecular Mass: 1,697.8269
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	6	4.3	4.7	7.3	5.2	5.5	9.4	6.7	7.2	15	15	15
y5 (1+)	6	4	4	6.4	4.3	5.1	8.8	5.9	6.5	15	15	15
y6 (1+)	5.7	4	4	9.2	4.3	4.7	10.8	5.9	6.2	15	15	15
sum	5.4	4	4.2	7.3	4.6	5.1	9.1	6.1	6.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-2884 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK13
Peptide Sequence: VENNLIVDK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 548
Peptide End: 556

CPTAC ID: non-CPTAC-2884
Peptide Molecular Mass: 1,042.5659
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend