CDK16, CPTAC-2936 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK16	PCTAIRE1, PCTK1

Sequence Length (AA)	Molecular Weight (Da)
496	55716

Protein Name
Cyclin-dependent kinase 16

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MDRMKKIKRQ	LSMTLRGGRG	IDKTNGAPEQ	IGLDESGGGG	GSDPGEAPTR
60	70	80	90	100
AAPGELRSAR	GPLSSAPEIV	HEDLKMGSDG	ESDQASATSS	DEVQSPVRVR
110	120	130	140	150
MRNHPPRKIS	TEDINKRLSL	PADIRLPEGY	LEKLTLNSPI	FDKPLSRRLR
160	170	180	190	200
RVSLSEIGFG	KLETYIKLDK	LGEGTYATVY	KGKSKLTDNL	VALKEIRLEH
210	220	230	240	250
EEGAPCTAIR	EVSLLKDLKH	ANIVTLHDII	HTEKSLTLVF	EYLDKDLKQY
260	270	280	290	300
LDDCGNIINM	HNVKLFLFQL	LRGLAYCHRQ	KVLHRDLKPQ	NLLINERGEL
310	320	330	340	350
KLADFGLARA	KSIPTKTYSN	EVVTLWYRPP	DILLGSTDYS	TQIDMWGVGC
360	370	380	390	400
IFYEMATGRP	LFPGSTVEEQ	LHFIFRILGT	PTEETWPGIL	SNEEFKTYNY
410	420	430	440	450
PKYRAEALLS	HAPRLDSDGA	DLLTKLLQFE	GRNRISAEDA	MKHPFFLSLG
460	470	480	490	496
ERIHKLPDTT	SIFALKEIQL	QKEASLRSSS	MPDSGRPAFR	VVDTEF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2936 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK16
Peptide Sequence: LDSDGADLLTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 415
Peptide End: 425

CPTAC ID: non-CPTAC-2936
Peptide Molecular Mass: 1,146.5768
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	13.1	6.5	7.8	20.1	7	8.4	24	9.6	11.5	15	15	15
y7 (1+)	10.1	4.6	8.1	9.6	5.3	9.9	13.9	7	12.8	15	15	15
y4 (1+)	16.1	9.7	10.2	15.7	10.1	10.9	22.5	14	14.9	15	15	15
sum	9.5	5.5	7.8	9.7	6.2	9.3	13.6	8.3	12.1	15	15	15

CDK16

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2936 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CDK16

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2936 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments