PAK5, CPTAC-2960 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
PAK5	KIAA1264, PAK7

Sequence Length (AA)	Molecular Weight (Da)
719	80745

Protein Name
Serine/threonine-protein kinase PAK 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MFGKKKKKIE	ISGPSNFEHR	VHTGFDPQEQ	KFTGLPQQWH	SLLADTANRP
60	70	80	90	100
KPMVDPSCIT	PIQLAPMKTI	VRGNKPCKET	SINGLLEDFD	NISVTRSNSL
110	120	130	140	150
RKESPPTPDQ	GASSHGPGHA	EENGFITFSQ	YSSESDTTAD	YTTEKYREKS
160	170	180	190	200
LYGDDLDPYY	RGSHAAKQNG	HVMKMKHGEA	YYSEVKPLKS	DFARFSADYH
210	220	230	240	250
SHLDSLSKPS	EYSDLKWEYQ	RASSSSPLDY	SFQFTPSRTA	GTSGCSKESL
260	270	280	290	300
AYSESEWGPS	LDDYDRRPKS	SYLNQTSPQP	TMRQRSRSGS	GLQEPMMPFG
310	320	330	340	350
ASAFKTHPQG	HSYNSYTYPR	LSEPTMCIPK	VDYDRAQMVL	SPPLSGSDTY
360	370	380	390	400
PRGPAKLPQS	QSKSGYSSSS	HQYPSGYHKA	TLYHHPSLQS	SSQYISTASY
410	420	430	440	450
LSSLSLSSST	YPPPSWGSSS	DQQPSRVSHE	QFRAALQLVV	SPGDPREYLA
460	470	480	490	500
NFIKIGEGST	GIVCIATEKH	TGKQVAVKKM	DLRKQQRREL	LFNEVVIMRD
510	520	530	540	550
YHHDNVVDMY	SSYLVGDELW	VVMEFLEGGA	LTDIVTHTRM	NEEQIATVCL
560	570	580	590	600
SVLRALSYLH	NQGVIHRDIK	SDSILLTSDG	RIKLSDFGFC	AQVSKEVPKR
610	620	630	640	650
KSLVGTPYWM	APEVISRLPY	GTEVDIWSLG	IMVIEMIDGE	PPYFNEPPLQ
660	670	680	690	700
AMRRIRDSLP	PRVKDLHKVS	SVLRGFLDLM	LVREPSQRAT	AQELLGHPFL
710	719
KLAGPPSCIV	PLMRQYRHH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2961 Collapse assay details

Data source: Panorama

Official Gene Symbol: PAK5
Peptide Sequence: SLYGDDLDPYYR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 150
Peptide End: 161

CPTAC ID: non-CPTAC-2961
Peptide Molecular Mass: 1,475.6569
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	10.3	10.3	20.7	11.5	12.1	55.5	15.4	15.9	59.2	15	15	15
y9 (1+)	5.3	5.1	14.1	5.6	9.6	15.1	7.7	10.9	20.7	15	15	15
y4 (1+)	3.4	3.8	9.7	5.6	6.2	10.3	6.6	7.3	14.1	15	15	15
sum	4.2	4.9	6.4	5.7	5.8	10.9	7.1	7.6	12.6	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-2960 Collapse assay details

Data source: Panorama

Official Gene Symbol: PAK5
Peptide Sequence: THPQGHSYNSYTYPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 306
Peptide End: 320

CPTAC ID: non-CPTAC-2960
Peptide Molecular Mass: 1,806.8074
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend