RIOK2, CPTAC-2990 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
RIOK2	N/A

Sequence Length (AA)	Molecular Weight (Da)
552	63283

Protein Name
Serine/threonine-protein kinase RIO2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGKVNVAKLR	YMSRDDFRVL	TAVEMGMKNH	EIVPGSLIAS	IASLKHGGCN
60	70	80	90	100
KVLRELVKHK	LIAWERTKTV	QGYRLTNAGY	DYLALKTLSS	RQVVESVGNQ
110	120	130	140	150
MGVGKESDIY	IVANEEGQQF	ALKLHRLGRT	SFRNLKNKRD	YHKHRHNVSW
160	170	180	190	200
LYLSRLSAMK	EFAYMKALYE	RKFPVPKPID	YNRHAVVMEL	INGYPLCQIH
210	220	230	240	250
HVEDPASVYD	EAMELIVKLA	NHGLIHGDFN	EFNLILDESD	HITMIDFPQM
260	270	280	290	300
VSTSHPNAEW	YFDRDVKCIK	DFFMKRFSYE	SELFPTFKDI	RREDTLDVEV
310	320	330	340	350
SASGYTKEMQ	ADDELLHPLG	PDDKNIETKE	GSEFSFSDGE	VAEKAEVYGS
360	370	380	390	400
ENESERNCLE	ESEGCYCRSS	GDPEQIKEDS	LSEESADARS	FEMTEFNQAL
410	420	430	440	450
EEIKGQVVEN	NSVTEFSEEK	NRTENYNRQD	GQRVQGGVPA	GSDEYEDECP
460	470	480	490	500
HLIALSSLNR	EFRPFRDEEN	VGAMNQYRTR	TLSITSSGSA	VSCSTIPPEL
510	520	530	540	550
VKQKVKRQLT	KQQKSAVRRR	LQKGEANIFT	KQRRENMQNI	KSSLEAASFW
552
GE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-2990 Collapse assay details

Data source: Panorama

Official Gene Symbol: RIOK2
Peptide Sequence: FSYESELFPTFK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 277
Peptide End: 288

CPTAC ID: non-CPTAC-2990
Peptide Molecular Mass: 1,493.7078
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	5.2	4	4.8	5.2	6.5	7.2	7.4	7.6	8.7	15	15	15
y8 (1+)	3.5	3.4	6.3	4.1	8.3	8.4	5.4	9	10.5	15	15	15
y4 (1+)	4.5	3	5.1	5.1	6.3	7	6.8	7	8.7	15	15	15
sum	3.9	3.1	5.2	4.5	6.7	7.3	6	7.4	9	15	15	15

RIOK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2990 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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RIOK2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-2990 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments