COQ8A, CPTAC-3019 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
COQ8A	ADCK3, CABC1

Sequence Length (AA)	Molecular Weight (Da)
647	71950

Protein Name
Atypical kinase COQ8A, mitochondrial

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAILGDTIM	VAKGLVKLTQ	AAVETHLQHL	GIGGELIMAA	RALQSTAVEQ
60	70	80	90	100
IGMFLGKVQG	QDKHEEYFAE	NFGGPEGEFH	FSVPHAAGAS	TDFSSASAPD
110	120	130	140	150
QSAPPSLGHA	HSEGPAPAYV	ASGPFREAGF	PGQASSPLGR	ANGRLFANPR
160	170	180	190	200
DSFSAMGFQR	RFFHQDQSPV	GGLTAEDIEK	ARQAKARPEN	KQHKQTLSEH
210	220	230	240	250
ARERKVPVTR	IGRLANFGGL	AVGLGFGALA	EVAKKSLRSE	DPSGKKAVLG
260	270	280	290	300
SSPFLSEANA	ERIVRTLCKV	RGAALKLGQM	LSIQDDAFIN	PHLAKIFERV
310	320	330	340	350
RQSADFMPLK	QMMKTLNNDL	GPNWRDKLEY	FEERPFAAAS	IGQVHLARMK
360	370	380	390	400
GGREVAMKIQ	YPGVAQSINS	DVNNLMAVLN	MSNMLPEGLF	PEHLIDVLRR
410	420	430	440	450
ELALECDYQR	EAACARKFRD	LLKGHPFFYV	PEIVDELCSP	HVLTTELVSG
460	470	480	490	500
FPLDQAEGLS	QEIRNEICYN	ILVLCLRELF	EFHFMQTDPN	WSNFFYDPQQ
510	520	530	540	550
HKVALLDFGA	TREYDRSFTD	LYIQIIRAAA	DRDRETVRAK	SIEMKFLTGY
560	570	580	590	600
EVKVMEDAHL	DAILILGEAF	ASDEPFDFGT	QSTTEKIHNL	IPVMLRHRLV
610	620	630	640	647
PPPEETYSLH	RKMGGSFLIC	SKLKARFPCK	AMFEEAYSNY	CKRQAQQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3020 Collapse assay details

Data source: Panorama

Official Gene Symbol: COQ8A
Peptide Sequence: AVLGSSPFLSEANAER
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 247
Peptide End: 262

CPTAC ID: non-CPTAC-3020
Peptide Molecular Mass: 1,646.8264
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y10 (1+)	5.3	3.9	4.6	6.9	4.1	5.3	8.7	5.7	7	15	15	15
y7 (1+)	4.5	3	4.4	5.6	3.7	4.1	7.2	4.8	6	15	15	15
y13 (1+)	4.7	3.9	4.2	8.6	5	5.6	9.8	6.3	7	15	15	15
sum	4.8	3.7	4.4	6.7	4.1	5	8.2	5.5	6.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3019 Collapse assay details

Data source: Panorama

Official Gene Symbol: COQ8A
Peptide Sequence: VALLDFGATR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 503
Peptide End: 512

CPTAC ID: non-CPTAC-3019
Peptide Molecular Mass: 1,061.5869
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend