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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
PIP4K2C PIP5K2C
Sequence Length (AA) Molecular Weight (Da)
421 47300
Protein Name
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASSSVPPAT VSAATAGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV
60 70 80 90 100
FLWGVAHSIN ELSQVPPPVM LLPDDFKASS KIKVNNHLFH RENLPSHFKF
110 120 130 140 150
KEYCPQVFRN LRDRFGIDDQ DYLVSLTRNP PSESEGSDGR FLISYDRTLV
160 170 180 190 200
IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT LLPQFLGMYR VSVDNEDSYM
210 220 230 240 250
LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL KDMDFLNKNQ
260 270 280 290 300
KVYIGEEEKK IFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEA
310 320 330 340 350
PVREDESEVD GDCSLTGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF
360 370 380 390 400
IDVYAIRSAE GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI
410 420 421
STVHPEQYAK RFLDFITNIF A

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3021 Collapse assay details

Data source: Panorama

Official Gene Symbol
PIP4K2C
Peptide Sequence
FGIDDQDYLVSLTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
115
Peptide End
128
CPTAC ID
non-CPTAC-3021
Peptide Molecular Mass
1,640.8046
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 5.1 5.3 7.1 5.8 5.3 8.6 7.7 7.5 11.2 15 15 15
y6 (1+) 4.4 4.8 5.4 5.1 4.7 7 6.7 6.7 8.8 15 15 15
y4 (1+) 3.8 4.1 5.1 4.2 4.3 6.2 5.7 5.9 8 15 15 15
sum 3.9 4.5 5.6 4.5 4.6 6.9 6 6.4 8.9 15 15 15


Additional Resources and Comments