MAPK15, CPTAC-3023 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
MAPK15	ERK7, ERK8

Sequence Length (AA)	Molecular Weight (Da)
544	59832

Protein Name
Mitogen-activated protein kinase 15

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MCTVVDPRIV	RRYLLRRQLG	QGAYGIVWKA	VDRRTGEVVA	IKKIFDAFRD
60	70	80	90	100
KTDAQRTFRE	ITLLQEFGDH	PNIISLLDVI	RAENDRDIYL	VFEFMDTDLN
110	120	130	140	150
AVIRKGGLLQ	DVHVRSIFYQ	LLRATRFLHS	GHVVHRDQKP	SNVLLDANCT
160	170	180	190	200
VKLCDFGLAR	SLGDLPEGPE	DQAVTEYVAT	RWYRAPEVLL	SSHRYTLGVD
210	220	230	240	250
MWSLGCILGE	MLRGRPLFPG	TSTLHQLELI	LETIPPPSEE	DLLALGSGCR
260	270	280	290	300
ASVLHQLGSR	PRQTLDALLP	PDTSPEALDL	LRRLLVFAPD	KRLSATQALQ
310	320	330	340	350
HPYVQRFHCP	SDEWAREADV	RPRAHEGVQL	SVPEYRSRVY	QMILECGGSS
360	370	380	390	400
GTSREKGPEG	VSPSQAHLHK	PRADPQLPSR	TPVQGPRPRP	QSSPGHDPAE
410	420	430	440	450
HESPRAAKNV	PRQNSAPLLQ	TALLGNGERP	PGAKEAPPLT	LSLVKPSGRG
460	470	480	490	500
AAPSLTSQAA	AQVANQALIR	GDWNRGGGVR	VASVQQVPPR	LPPEARPGRR
510	520	530	540	544
MFSTSALQGA	QGGARALLGG	YSQAYGTVCH	SALGHLPLLE	GHHV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for non-CPTAC-3023 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK15
Peptide Sequence: APEVLLSSHR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 185
Peptide End: 194

CPTAC ID: non-CPTAC-3023
Peptide Molecular Mass: 1,107.6037
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: ThermoFisher, Q-Exactive
Internal Standard: 25 fmol on column
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Easy-nLC 1000 Thermo Scientific
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	7.5	7.5	5.9	9.3	8.2	7.4	11.9	11.1	9.5	15	15	15
y5 (1+)	7.5	6.7	6	8.7	7.5	6.5	11.5	10.1	8.8	15	15	15
y6 (1+)	8.2	6.5	5.5	8.8	7.8	7.5	12	10.2	9.3	15	15	15
sum	7.2	6.6	5.4	8.2	7.6	6.8	10.9	10.1	8.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3022 Collapse assay details

Data source: Panorama

Official Gene Symbol: MAPK15
Peptide Sequence: MFSTSALQGAQGGAR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 501
Peptide End: 515

CPTAC ID: non-CPTAC-3022
Peptide Molecular Mass: 1,480.7093
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend