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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

MARK3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MARK3 CTAK1, EMK2
Sequence Length (AA) Molecular Weight (Da)
753 84489
Protein Name
MAP/microtubule affinity-regulating kinase 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTRTPLPTV NERDTENHTS HGDGRQEVTS RTSRSGARCR NSIASCADEQ
60 70 80 90 100
PHIGNYRLLK TIGKGNFAKV KLARHILTGR EVAIKIIDKT QLNPTSLQKL
110 120 130 140 150
FREVRIMKIL NHPNIVKLFE VIETEKTLYL IMEYASGGEV FDYLVAHGRM
160 170 180 190 200
KEKEARSKFR QIVSAVQYCH QKRIVHRDLK AENLLLDADM NIKIADFGFS
210 220 230 240 250
NEFTVGGKLD TFCGSPPYAA PELFQGKKYD GPEVDVWSLG VILYTLVSGS
260 270 280 290 300
LPFDGQNLKE LRERVLRGKY RIPFYMSTDC ENLLKRFLVL NPIKRGTLEQ
310 320 330 340 350
IMKDRWINAG HEEDELKPFV EPELDISDQK RIDIMVGMGY SQEEIQESLS
360 370 380 390 400
KMKYDEITAT YLLLGRKSSE LDASDSSSSS NLSLAKVRPS SDLNNSTGQS
410 420 430 440 450
PHHKVQRSVF SSQKQRRYSD HAGPAIPSVV AYPKRSQTST ADSDLKEDGI
460 470 480 490 500
SSRKSSGSAV GGKGIAPASP MLGNASNPNK ADIPERKKSS TVPSSNTASG
510 520 530 540 550
GMTRRNTYVC SERTTADRHS VIQNGKENST IPDQRTPVAS THSISSAATP
560 570 580 590 600
DRIRFPRGTA SRSTFHGQPR ERRTATYNGP PASPSLSHEA TPLSQTRSRG
610 620 630 640 650
STNLFSKLTS KLTRRNMSFR FIKRLPTEYE RNGRYEGSSR NVSAEQKDEN
660 670 680 690 700
KEAKPRSLRF TWSMKTTSSM DPGDMMREIR KVLDANNCDY EQRERFLLFC
710 720 730 740 750
VHGDGHAENL VQWEMEVCKL PRLSLNGVRF KRISGTSIAF KNIASKIANE
753
LKL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3197 Collapse assay details

Data source: Panorama

Official Gene Symbol
MARK3
Peptide Sequence
TPVASTHSISSAATPDR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
536
Peptide End
552
CPTAC ID
non-CPTAC-3197
Peptide Molecular Mass
1,696.8380
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.3 3.4 4.2 6.8 6.3 4.6 9.3 7.2 6.2 15 15 15
y8 (1+) 5.1 4.4 3.4 5.1 6 4.2 7.2 7.4 5.4 15 15 15
y7 (1+) 7.2 3.5 3.9 6.3 6.4 6.4 9.6 7.3 7.5 15 15 15
sum 5.5 3.6 3 5.1 5.8 4.4 7.5 6.8 5.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3196 Collapse assay details

Data source: Panorama

Official Gene Symbol
MARK3
Peptide Sequence
YDEITATYLLLGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
354
Peptide End
366
CPTAC ID
non-CPTAC-3196
Peptide Molecular Mass
1,526.7981
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 3.9 2.6 3.2 7.6 7.5 9.7 8.5 7.9 10.2 15 15 15
y8 (1+) 4.6 3.2 5 6.6 9.5 12.1 8 10 13.1 15 15 15
y9 (1+) 6 2.8 4.3 7.3 9.6 11.8 9.4 10 12.6 15 15 15
sum 4.7 2.8 4 7.1 8.9 11.2 8.5 9.3 11.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments