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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Igfbp3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Igfbp3 Igfbp-3
Sequence Length (AA) Molecular Weight (Da)
292 31687
Protein Name
Insulin-like growth factor-binding protein 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MHPARPALWA AALTALTLLR GPPVARAGAG AVGAGPVVRC EPCDARALSQ
60 70 80 90 100
CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP
110 120 130 140 150
AEQYPLRALL NGRGFCANAS AAGSLSTYLP SQPAPGNISE SEEEHNAGSV
160 170 180 190 200
ESQVVPSTHR VTDSKFHPLH AKMDVIKKGH ARDSQRYKVD YESQSTDTQN
210 220 230 240 250
FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK
260 270 280 290 292
QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3300 Collapse assay details

Data source: Panorama

Official Gene Symbol
Igfbp3
Peptide Sequence
FHPLHAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
166
Peptide End
172
CPTAC ID
non-CPTAC-3300
Peptide Molecular Mass
848.4657
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 11.7 11.1 13.3 22.9 28.3 12.6 25.7 30.4 18.3 15 15 15
y6 (2+) 15.6 8.7 7.2 15.8 10.9 8.9 22.2 13.9 11.4 15 15 15
b2 (1+) 12.5 11.5 11.5 15.1 13.2 13.2 19.6 17.5 17.5 15 15 15
b6 (3+) 12 10.9 15 15.3 22.3 26.8 19.4 24.8 30.7 15 15 15
y2 (1+) 7.9 9.6 14 16.5 13.2 13.5 18.3 16.3 19.4 15 15 15
sum 5.9 5 5.2 10.9 9.5 7.3 12.4 10.7 9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments