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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Gp5

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Gp5 N/A
Sequence Length (AA) Molecular Weight (Da)
567 63468
Protein Name
Platelet glycoprotein V
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLRSALLSAV LPLLRAQPFP CPKTCKCVVR DAAQCSGGSV AHIAELGLPT
60 70 80 90 100
NLTHILLFRM DQGILRNHSF SGMTVLQRQM LSDSHISAID PGTFNDLVKL
110 120 130 140 150
KTLRLTRNKI SRLPRAILDK MVLLEQLFLD HNALRDLDQN LFQQLRNLQE
160 170 180 190 200
LGLNQNQLSF LPANLFSSLR ELKLLDLSRN NLTHLPKGLL GAQVKLEKLL
210 220 230 240 250
LYSNQLTSVD SGLLSNLGAL TELRLERNHL RSVAPGAFDR LGNLSSLTLS
260 270 280 290 300
GNLLESLPPA LFLHVSSVSR LTLFENPLEE LPDVLFGEMA GLRELWLNGT
310 320 330 340 350
HLSTLPAAAF RNLSGLQTLG LTRNPRLSAL PRGVFQGLRE LRVLGLHTNA
360 370 380 390 400
LAELRDDALR GLGHLRQVSL RHNRLRALPR TLFRNLSSLE SVQLEHNQLE
410 420 430 440 450
TLPGDVFAAL PQLTQVLLGH NPWLCDCGLW RFLQWLRHHP DILGRDEPPQ
460 470 480 490 500
CRGPEPRASL SFWELLQGDP WCPDPRSLPL DPPTENALEA PVPSWLPNSW
510 520 530 540 550
QSQTWAQLVA RGESPNNRLY WGLYILLLVA QAIIAAFIVF AMIKIGQLFR
560 567
TLIREKLLLE AMGKSCN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3306 Collapse assay details

Data source: Panorama

Official Gene Symbol
Gp5
Peptide Sequence
HHPDILGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
438
Peptide End
445
CPTAC ID
non-CPTAC-3306
Peptide Molecular Mass
943.4988
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 11.3 4.8 6.9 17.3 8.3 5.9 20.7 9.6 9.1 15 15 15
y2 (1+) 18.6 6.1 7 21.4 13.5 8.6 28.4 14.8 11.1 15 15 15
b4 (2+) 12.2 4 6.6 17 8.2 8.2 20.9 9.1 10.5 15 15 15
y6 (2+) 8.6 7.1 7.3 11.8 9.1 7.9 14.6 11.5 10.8 15 15 15
y3 (1+) 13 3.7 3.1 14.4 11.8 3.1 19.4 12.4 4.4 15 15 15
sum 6 1.8 3.1 13.1 8.8 3.8 14.4 9 4.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments