Grin2a, CPTAC-3342 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Grin2a	N/A

Sequence Length (AA)	Molecular Weight (Da)
1464	165421

Protein Name
Glutamate receptor ionotropic, NMDA 2A

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGRLGYWTLL	VLPALLVWHG	PAQNAAAEKG	TPALNIAVLL	GHSHDVTERE
60	70	80	90	100
LRNLWGPEQA	TGLPLDVNVV	ALLMNRTDPK	SLITHVCDLM	SGARIHGLVF
110	120	130	140	150
GDDTDQEAVA	QMLDFISSQT	FIPILGIHGG	ASMIMADKDP	TSTFFQFGAS
160	170	180	190	200
IQQQATVMLK	IMQDYDWHVF	SLVTTIFPGY	RDFISFIKTT	VDNSFVGWDM
210	220	230	240	250
QNVITLDTSF	EDAKTQVQLK	KIHSSVILLY	CSKDEAVLIL	SEARSLGLTG
260	270	280	290	300
YDFFWIVPSL	VSGNTELIPK	EFPSGLISVS	YDDWDYSLEA	RVRDGLGILT
310	320	330	340	350
TAASSMLEKF	SYIPEAKASC	YGQTEKPETP	LHTLHQFMVN	VTWDGKDLSF
360	370	380	390	400
TEEGYQVHPR	LVVIVLNKDR	EWEKVGKWEN	QTLSLRHAVW	PRYKSFSDCE
410	420	430	440	450
PDDNHLSIVT	LEEAPFVIVE	DIDPLTETCV	RNTVPCRKFV	KINNSTNEGM
460	470	480	490	500
NVKKCCKGFC	IDILKKLSRT	VKFTYDLYLV	TNGKHGKKVN	NVWNGMIGEV
510	520	530	540	550
VYQRAVMAVG	SLTINEERSE	VVDFSVPFVE	TGISVMVSRS	NGTVSPSAFL
560	570	580	590	600
EPFSASVWVM	MFVMLLIVSA	IAVFVFEYFS	PVGYNRNLAK	GKAPHGPSFT
610	620	630	640	650
IGKAIWLLWG	LVFNNSVPVQ	NPKGTTSKIM	VSVWAFFAVI	FLASYTANLA
660	670	680	690	700
AFMIQEEFVD	QVTGLSDKKF	QRPHDYSPPF	RFGTVPNGST	ERNIRNNYPY
710	720	730	740	750
MHQYMTKFNQ	RGVEDALVSL	KTGKLDAFIY	DAAVLNYKAG	RDEGCKLVTI
760	770	780	790	800
GSGYIFATTG	YGIALQKGSP	WKRQIDLALL	QFVGDGEMEE	LETLWLTGIC
810	820	830	840	850
HNEKNEVMSS	QLDIDNMAGV	FYMLAAAMAL	SLITFIWEHL	FYWKLRFCFT
860	870	880	890	900
GVCSDRPGLL	FSISRGIYSC	IHGVHIEEKK	KSPDFNLTGS	QSNMLKLLRS
910	920	930	940	950
AKNISNMSNM	NSSRMDSPKR	AADFIQRGSL	IVDMVSDKGN	LIYSDNRSFQ
960	970	980	990	1000
GKDSIFGENM	NELQTFVANR	HKDSLSNYVF	QGQHPLTLNE	SNPNTVEVAV
1010	1020	1030	1040	1050
STESKGNSRP	RQLWKKSMES	LRQDSLNQNP	VSQRDEKTAE	NRTHSLKSPR
1060	1070	1080	1090	1100
YLPEEVAHSD	ISETSSRATC	HREPDNNKNH	KTKDNFKRSM	ASKYPKDCSE
1110	1120	1130	1140	1150
VERTYVKTKA	SSPRDKIYTI	DGEKEPSFHL	DPPQFIENIV	LPENVDFPDT
1160	1170	1180	1190	1200
YQDHNENFRK	GDSTLPMNRN	PLHNEDGLPN	NDQYKLYAKH	FTLKDKGSPH
1210	1220	1230	1240	1250
SEGSDRYRQN	STHCRSCLSN	LPTYSGHFTM	RSPFKCDACL	RMGNLYDIDE
1260	1270	1280	1290	1300
DQMLQETGNP	ATREEAYQQD	WSQNNALQFQ	KNKLKINRQH	SYDNILDKPR
1310	1320	1330	1340	1350
EIDLSRPSRS	ISLKDRERLL	EGNLYGSLFS	VPSSKLLGNK	SSLFPQGLED
1360	1370	1380	1390	1400
SKRSKSLLPD	HTSDNPFLHT	YGDDQRLVIG	RCPSDPYKHS	LPSQAVNDSY
1410	1420	1430	1440	1450
LRSSLRSTAS	YCSRDSRGHS	DVYISEHVMP	YAANKNNMYS	TPRVLNSCSN
1460	1464
RRVYKKMPSI	ESDV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3342 Collapse assay details

Data source: Panorama

Official Gene Symbol: Grin2a
Peptide Sequence: DLSFTEEGYQVHPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 347
Peptide End: 360

CPTAC ID: non-CPTAC-3342
Peptide Molecular Mass: 1,676.7795
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	4.4	5.5	4.3	15.1	12.2	5.9	15.7	13.4	7.3	15	15	15
y10 (2+)	4.5	8	5.4	16.5	11.7	5.8	17.1	14.2	7.9	15	15	15
y11 (2+)	7.1	9.2	6.9	11	9	7.5	13.1	12.9	10.2	15	15	15
y12 (2+)	5.6	5.9	6.2	16.5	10.8	6.1	17.4	12.3	8.7	15	15	15
y2 (1+)	3.6	5.7	7.6	17.4	18.5	5.7	17.8	19.4	9.5	15	15	15
sum	2.2	3.8	3.1	15.3	10.6	3.7	15.5	11.3	4.8	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3341 Collapse assay details

Data source: Panorama

Official Gene Symbol: Grin2a
Peptide Sequence: FSYIPEAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 310
Peptide End: 317

CPTAC ID: non-CPTAC-3341
Peptide Molecular Mass: 953.4858
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers