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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cpn1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cpn1 N/A
Sequence Length (AA) Molecular Weight (Da)
457 51845
Protein Name
Carboxypeptidase N catalytic chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPDLPSAFLP LLLLSKFVTP VTFRHHRYDD LVRTLYKVHN QCPDITRLYN
60 70 80 90 100
IGRSVKGRYL YVLEFSDYPG IHEPLEPEVK YVGNMHGNEV LGRELLLQLS
110 120 130 140 150
EFLCEEFRNR NQRILRLIQD TRIHILPSMN PDGYEVAAAQ GPNMSGYLVG
160 170 180 190 200
RNNANGVDLN RNFPDLNTYF YYNSKNGGPN HHLPLPDNWK SQVEPETRAV
210 220 230 240 250
IQWIRSLNFV LSANMHGGAV VANYPYDKSL EHRFRGPHRT SNSPTPDDEL
260 270 280 290 300
FQTLAKVYSY AHGWMHQGWN CGDYFPDGIT NGASWYSLSK GMQDFNYLHT
310 320 330 340 350
NCFEITLELS CDKFPRQEEL QREWLGNREA LIQFLEQVHQ GIKGMVLDEN
360 370 380 390 400
SNNLTGAVIS VTGINHDVTS GEHGDYFRLL LPGTYSVTAK APGYDPKTVT
410 420 430 440 450
VTVGPAGPTV VDFQLKRSSS QVYPVQRAPG RGQGGRAKQP RTSRKKDPAT
457
KRHRGPA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3344 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cpn1
Peptide Sequence
EALIQFLEQVHQGIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
329
Peptide End
343
CPTAC ID
non-CPTAC-3344
Peptide Molecular Mass
1,751.9570
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (3+) 6.2 7.6 6.6 17.9 13.6 12.3 18.9 15.6 14 15 15 15
y7 (2+) 10 13.8 7.2 15.6 17.1 11.3 18.5 22 13.4 15 15 15
y10 (2+) 2.9 3.6 4.6 15.5 5.5 8.1 15.8 6.6 9.3 15 15 15
y11 (2+) 6.6 5.1 4 10.5 8.6 9.3 12.4 10 10.1 15 15 15
y12 (2+) 8.9 8.2 4.5 15.4 9.2 8.7 17.8 12.3 9.8 15 15 15
sum 3.1 2.4 2.1 12.9 6.7 8 13.3 7.1 8.3 15 15 15

Additional Resources and Comments

Resources
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View Peptide in Panorama
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Comments

Assay Details for non-CPTAC-3696 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cpn1
Peptide Sequence
NNANGVDLNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
152
Peptide End
161
CPTAC ID
non-CPTAC-3696
Peptide Molecular Mass
1,085.5214
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.4 10.8 11.6 14.1 18.1 13.7 17.5 21.1 18 15 15 15
b3 (1+) 13.2 14.4 7.6 13 12.2 10.7 18.5 18.9 13.1 15 15 15
b2 (1+) 15.8 10.5 9.3 12.4 11.5 10.3 20.1 15.6 13.9 15 15 15
y3 (1+) 16.3 7.5 7.7 13.9 11.8 9.9 21.4 14 12.5 15 15 15
y4 (1+) 19.2 17.8 13.9 25.1 17.3 16.6 31.6 24.8 21.7 15 15 15
sum 11.1 6.4 4.9 11.3 7.9 7.7 15.8 10.2 9.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments