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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Tnc

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Tnc Hxb
Sequence Length (AA) Molecular Weight (Da)
2110 231807
Protein Name
Tenascin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGAVTWLLPG IFLALFALTP EGGVLKKIIR HKRESGLNMT LPEENQPVVF
60 70 80 90 100
NHIYNIKLPM GSQCSVDLES ASGEKDLTPT PESSGSFQEH TVDGENQIVF
110 120 130 140 150
THRINIPRRA CGCAAAPDVK ELLSRLEELE LLVSSLREQC TMGTGCCLQP
160 170 180 190 200
AEGRLDTRPF CSGRGNFSAE GCGCVCEPGW KGPNCSEPDC PGNCNLRGQC
210 220 230 240 250
LDGQCICDEG FTGEDCSQLA CPNDCNDQGR CVNGVCVCFE GYAGPDCGLE
260 270 280 290 300
VCPVPCSEEH GMCVDGRCVC KDGFAGEDCN EPLCLNNCYN RGRCVENECV
310 320 330 340 350
CDEGFTGEDC SELICPNDCF DRGRCINGTC YCEEGFTGED CGELTCPNDC
360 370 380 390 400
QGRGQCEEGQ CVCNEGFAGA DCSEKRCPAD CHHRGRCLNG QCECDDGFTG
410 420 430 440 450
ADCGDLQCPN GCSGHGRCVN GQCVCDEGYT GEDCSQRRCP NDCHNRGLCV
460 470 480 490 500
QGKCICEQGF KGFDCSEMSC PNDCHQHGRC VNGMCICDDD YTGEDCRDRR
510 520 530 540 550
CPRDCSQRGR CVDGQCICED GFTGPDCAEL SCPSDCHGHG RCVNGQCICH
560 570 580 590 600
EGFTGKDCKE QRCPSDCHGQ GRCEDGQCIC HEGFTGLDCG QRSCPNDCSN
610 620 630 640 650
QGQCVSGRCI CNEGYTGIDC SEVSPPKDLI VTEVTEETVN LAWDNEMRVT
660 670 680 690 700
EYLIMYTPTH ADGLEMQFRV PGDQTSTTIR ELEPGVEYFI RVFAILENKR
710 720 730 740 750
SIPVSARVAT YLPAPEGLKF KSIKETSVEV EWDPLDIAFE TWEIIFRNMN
760 770 780 790 800
KEDEGEITKS LRRPETSYRQ TGLAPGQEYE ISLHIVKNNT RGPGLKKVTT
810 820 830 840 850
TRLDAPSHIE VKDVTDTTAL ITWFKPLAEI DSIELSYGIK DVPGDRTTID
860 870 880 890 900
LTHEDNQYSI GNLRPDTEYE VSLISRRVDM ASNPAKETFI TGLDAPRNLR
910 920 930 940 950
RVSQTDNSIT LEWRNVKADI DSYRIKYAPI SGGDHAEIDV PKSQQATTKT
960 970 980 990 1000
TLTGLRPGTE YGIGVSAVKG DKESDPATIN AATEIDAPKD LRVSETTQDS
1010 1020 1030 1040 1050
LTFFWTTPLA KFDRYRLNYS LPTGQSMEVQ LPKDATSHVL TDLEPGQEYT
1060 1070 1080 1090 1100
VLLIAEKGRH KSKPARVKAS TEEVPSLENL TVTEAGWDGL RLNWTADDLA
1110 1120 1130 1140 1150
YEYFVIQVQE ANNVETAHNF TVPGNLRAAD IPGLKVATSY RVSIYGVARG
1160 1170 1180 1190 1200
YRTPVLSAET STGTTPNLGE VTVAEVGWDA LTLNWTAPEG AYKNFFIQVL
1210 1220 1230 1240 1250
EADTTQTVQN LTVPGGLRSV DLPGLKAATR YYITLRGVTQ DFGTAPLSVE
1260 1270 1280 1290 1300
VLTEDLPQLG GLSVTEVSWD GLTLNWTTDD LAYKHFVVQV QEANNVEAAQ
1310 1320 1330 1340 1350
NLTVPGSLRA VDIPGLKADT PYRVSIYGVI QGYRTPMLST DVSTAREPEI
1360 1370 1380 1390 1400
GNLNVSDVTP KSFNLSWTAT DGIFDMFTIE IIDSNRLLQT AEHNISGAER
1410 1420 1430 1440 1450
TAHISGLPPS TDFIVYLSGI APSIRTKTIS TTATTEALPL LENLTISDTN
1460 1470 1480 1490 1500
PYGFTVSWTA SENAFDSFLV TVVDSGKLLD PQEFTLSGTQ RKLELRGLIT
1510 1520 1530 1540 1550
GIGYEVLVSG FTQGHQTKPL RAETITEAEP EVDNLLVSDA TPDGFRLSWT
1560 1570 1580 1590 1600
ADEGIFDSFV IRIRDTKKQS EPQEISLPSP ERTRDITGLR EATEYEIELY
1610 1620 1630 1640 1650
GISRGRRSQP VSAIATTAMG SPKEIMFSDI TENAATVSWR APTAQVESFR
1660 1670 1680 1690 1700
ITYVPMTGGA PSMVTVDGTD TETRLVKLTP GVEYRVSVIA MKGFEESDPV
1710 1720 1730 1740 1750
SGTLITALDG PSGLLIANIT DSEALAMWQP AIATVDSYVI SYTGERVPEV
1760 1770 1780 1790 1800
TRTVSGNTVE YELHDLEPAT EYILSIFAEK GQQKSSTIAT KFTTDLDSPR
1810 1820 1830 1840 1850
EFTATEVQSE TALLTWRPPR ASVTGYLLVY ESVDGTVKEV IVGPDTTSYS
1860 1870 1880 1890 1900
LADLSPSTHY SARIQALSGS LRSKLIQTIF TTIGLLYPFP RDCSQAMLNG
1910 1920 1930 1940 1950
DTTSGLYTIY INGDKTQALE VYCDMTSDGG GWIVFLRRKN GREDFYRNWK
1960 1970 1980 1990 2000
AYAAGFGDRR EEFWLGLDNL SKITAQGQYE LRVDLQDHGE SAYAVYDRFS
2010 2020 2030 2040 2050
VGDAKSRYKL KVEGYSGTAG DSMNYHNGRS FSTYDKDTDS AITNCALSYK
2060 2070 2080 2090 2100
GAFWYKNCHR VNLMGRYGDN NHSQGVNWFH WKGHEYSIQF AEMKLRPSNF
2110
RNLEGRRKRA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-2700 Collapse assay details

Data source: Panorama

Official Gene Symbol
TNC
Peptide Sequence
FTTDLDSPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1792
Peptide End
1800
CPTAC ID
non-CPTAC-2700
Peptide Molecular Mass
1,050.4982
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 13.2 6 6.5 17.6 11.1 10.3 22 12.6 12.2 15 15 15
y4 (1+) 15.5 5.9 8.4 21.9 10.1 10 26.8 11.7 13.1 15 15 15
y7 (1+) 8.8 5.6 5.6 14.5 7.5 7.5 17 9.4 9.4 15 15 15
y3 (1+) 15.3 7.4 5.7 18.3 9 7.3 23.9 11.7 9.3 15 15 15
b2 (1+) 14.2 4.3 5.7 21.8 6.2 7.2 26 7.5 9.2 15 15 15
sum 5.6 3.7 5.2 12.2 7.5 7.5 13.4 8.4 9.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3359 Collapse assay details

Data source: Panorama

Official Gene Symbol
Tnc
Peptide Sequence
FTTDLDSPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1792
Peptide End
1800
CPTAC ID
non-CPTAC-3359
Peptide Molecular Mass
1,050.4982
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 23.3 11 7.6 24.7 12 9.3 34 16.3 12 15 15 15
y3 (1+) 7.3 7.4 13.6 14.4 11 9.8 16.1 13.3 16.8 15 15 15
y4 (1+) 12.4 10.8 12.1 19.4 13.1 12.8 23 17 17.6 15 15 15
y5 (1+) 10.1 8.4 8.8 22.5 8.8 10 24.7 12.2 13.3 15 15 15
y7 (1+) 9.8 6.4 8.8 13.6 10.5 8.7 16.8 12.3 12.4 15 15 15
sum 3.9 4.7 4.5 13 7.4 5.7 13.6 8.8 7.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments