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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Fetub N/A
Sequence Length (AA) Molecular Weight (Da)
388 42713
Protein Name
Fetuin-B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGLLRLLVLC TLAACCMARS PPAPPLPQRP LSPLHPLGCN DSEVLAVAGF
60 70 80 90 100
ALQNINRDQK DGYMLSLNRV HDVREHYQED MGSLFYLTLD VLETDCHVLS
110 120 130 140 150
RKAQKDCKPR MFYESVYGQC KAMFHINKPR RVLYLPAYNC TLRPVSKRKT
160 170 180 190 200
HTTCPDCPSP IDLSNPSALE AATESLAKFN SKSPSKKYEL VKVTKAMNQW
210 220 230 240 250
VSGPAYYVEY LIKEAPCTKS QASCSLQHSD SEPVGICQGS TVQSSLRHVP
260 270 280 290 300
LIQPVEKSVT VTCEFFESQA QVPGDENPAV TQGPQKLPQK NTAPTSSPSV
310 320 330 340 350
TAPRGSIQHL PELDDEKPEE SKGGSPEEAF PVQLDLTTNP QGDTLDVSFL
360 370 380 388
YLEPGDKKLV VLPFPGKEQR SAECPGPEKE NNPLVLPP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3371 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fetub
Peptide Sequence
HVPLIQPVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
248
Peptide End
257
CPTAC ID
non-CPTAC-3371
Peptide Molecular Mass
1,158.6761
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 3.8 4.4 3.3 15 6.9 7.8 15.5 8.2 8.5 15 15 15
b4 (1+) 3.8 3.2 4.1 12.9 3.8 9.6 13.4 5 10.4 15 15 15
b2 (1+) 2.9 2.6 3.7 14.4 5.3 7.4 14.7 5.9 8.3 15 15 15
y7 (3+) NA NA NA NA NA NA NA NA NA 0 0 0
y4 (1+) 3.5 3.2 2.7 13.2 6.3 5.7 13.7 7.1 6.3 15 15 15
y2 (1+) NA NA NA NA NA NA NA NA NA 0 0 0
sum 1.9 1.8 2.1 13.3 4.8 6.7 13.4 5.1 7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1116 Collapse assay details

Data source: Panorama

Official Gene Symbol
FETUB
Peptide Sequence
LVVLPFPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
353
Peptide End
360
CPTAC ID
non-CPTAC-1116
Peptide Molecular Mass
911.5844
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.4 5.5 4 7 7.5 5.7 9.5 9.3 7 15 15 15
b2 (1+) 3 7 5.6 5.6 6.2 6.8 6.4 9.4 8.8 15 15 15
y2 (1+) 5.8 4.8 4.5 6.3 6.1 5.1 8.6 7.8 6.8 15 15 15
y4 (1+) 5.8 6.9 5.9 6.9 7.4 5.4 9 10.1 8 15 15 15
y6 (1+) 4.4 7.9 4.4 5.7 8.2 4.9 7.2 11.4 6.6 15 15 15
sum 2.6 5.3 2.4 5 5.6 3.1 5.6 7.7 3.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3372 Collapse assay details

Data source: Panorama

Official Gene Symbol
Fetub
Peptide Sequence
NTAPTSSPSVTAPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
291
Peptide End
304
CPTAC ID
non-CPTAC-3372
Peptide Molecular Mass
1,384.6947
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6 8.6 7.4 13.7 13.5 9.3 15 16 11.9 15 15 15
y11 (2+) 12.5 10.2 8.2 16.5 17.6 9.5 20.7 20.3 12.5 15 15 15
y12 (2+) 15.7 6.7 10.5 23 9.4 12.9 27.8 11.5 16.6 15 15 15
y7 (1+) 17.2 7.7 7.7 31 12.1 12.8 35.5 14.3 14.9 15 15 15
y11 (1+) 10 11.7 7.9 17.8 12.1 7 20.4 16.8 10.6 15 15 15
sum 4.2 5.2 5 15.1 10 6 15.7 11.3 7.8 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1115 Collapse assay details

Data source: Panorama

Official Gene Symbol
FETUB
Peptide Sequence
VNDAQEYR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
67
Peptide End
74
CPTAC ID
non-CPTAC-1115
Peptide Molecular Mass
993.4516
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 18.8 6.4 5.1 23.7 6.3 4.4 30.3 9 6.7 12 13 15
y2 (1+) 12.7 9.4 6.7 12.1 10.8 7 17.5 14.3 9.7 14 15 15
y5 (1+) 12.5 5.4 6.7 13.6 7.7 6.9 18.5 9.4 9.6 13 15 15
y6 (1+) 8.6 8.2 5.3 19.1 7.9 5.2 20.9 11.4 7.4 4 15 15
y7 (1+) 14.1 6.8 5.1 20.8 9.1 6.4 25.1 11.4 8.2 13 15 15
sum 14.5 5 3.5 14.9 5 3.1 20.8 7.1 4.7 15 15 15

Additional Resources and Comments