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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Hsp90ab1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hsp90ab1 Hsp84, Hsp84-1, Hspcb
Sequence Length (AA) Molecular Weight (Da)
724 83281
Protein Name
Heat shock protein HSP 90-beta
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPEEVHHGEE EVETFAFQAE IAQLMSLIIN TFYSNKEIFL RELISNASDA
60 70 80 90 100
LDKIRYESLT DPSKLDSGKE LKIDIIPNPQ ERTLTLVDTG IGMTKADLIN
110 120 130 140 150
NLGTIAKSGT KAFMEALQAG ADISMIGQFG VGFYSAYLVA EKVVVITKHN
160 170 180 190 200
DDEQYAWESS AGGSFTVRAD HGEPIGRGTK VILHLKEDQT EYLEERRVKE
210 220 230 240 250
VVKKHSQFIG YPITLYLEKE REKEISDDEA EEEKGEKEEE DKEDEEKPKI
260 270 280 290 300
EDVGSDEEDD SGKDKKKKTK KIKEKYIDQE ELNKTKPIWT RNPDDITQEE
310 320 330 340 350
YGEFYKSLTN DWEDHLAVKH FSVEGQLEFR ALLFIPRRAP FDLFENKKKK
360 370 380 390 400
NNIKLYVRRV FIMDSCDELI PEYLNFIRGV VDSEDLPLNI SREMLQQSKI
410 420 430 440 450
LKVIRKNIVK KCLELFSELA EDKENYKKFY EAFSKNLKLG IHEDSTNRRR
460 470 480 490 500
LSELLRYHTS QSGDEMTSLS EYVSRMKETQ KSIYYITGES KEQVANSAFV
510 520 530 540 550
ERVRKRGFEV VYMTEPIDEY CVQQLKEFDG KSLVSVTKEG LELPEDEEEK
560 570 580 590 600
KKMEESKAKF ENLCKLMKEI LDKKVEKVTI SNRLVSSPCC IVTSTYGWTA
610 620 630 640 650
NMERIMKAQA LRDNSTMGYM MAKKHLEINP DHPIVETLRQ KAEADKNDKA
660 670 680 690 700
VKDLVVLLFE TALLSSGFSL EDPQTHSNRI YRMIKLGLGI DEDEVTAEEP
710 720 724
SAAVPDEIPP LEGDEDASRM EEVD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-6097 Collapse assay details

Data source: Panorama

Official Gene Symbol
HSP90AB1
Peptide Sequence
VVNVSSIMSVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
11
CPTAC ID
non-CPTAC-6097
Peptide Molecular Mass
1,189.6489
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
Bovine Serum Albumin
Submitting Laboratory
McGill University
Submitting Lab PI
Christoph H. Borchers, Gerald Batist

Publication

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mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6495B QQQ-MS
Internal Standard
Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Infinity 1290
Column Packing
commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions
4.6 mm × 150 mm, (5 µm)
Flow Rate
0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 5.9 3.2 3.3 5.7 3.6 5.1 8.2 4.8 6.1 16 16 17
y6 (1+) 6 3 4.3 5.2 4.6 4.9 7.9 5.5 6.5 16 16 17
y7 (1+) 5 4 3.5 4.9 4.1 4.8 7 5.7 5.9 16 16 17
y8 (1+) 9.9 4.4 3.3 6 5.1 5.4 11.6 6.7 6.3 16 16 17
y9 (1+) 4.1 4.8 3.7 4.7 4 4 6.2 6.2 5.4 16 16 17
sum 2.9 1.9 1.7 1.8 1.6 2.1 3.4 2.5 2.7 16 16 17

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-3376 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hsp90ab1
Peptide Sequence
IDIIPNPQER
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
73
Peptide End
82
CPTAC ID
non-CPTAC-3376
Peptide Molecular Mass
1,193.6404
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (1+) 9.9 6.5 7 16.7 10.8 7.7 19.4 12.6 10.4 15 15 15
b3 (1+) 7.2 5.7 5.8 12.6 8.6 5.5 14.5 10.3 8 15 15 15
y6 (2+) 8.1 5 7.1 16.1 9.8 10.4 18 11 12.6 15 15 15
y4 (1+) 23 6.4 6.9 30.1 9.9 8.2 37.9 11.8 10.7 15 15 15
y6 (1+) 6.4 3.2 4.3 12.6 7.6 6.1 14.1 8.2 7.5 15 15 15
sum 4.5 2.9 4.6 11.9 7.5 4.6 12.7 8 6.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3377 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hsp90ab1
Peptide Sequence
LGIHEDSTNR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
439
Peptide End
448
CPTAC ID
non-CPTAC-3377
Peptide Molecular Mass
1,140.5524
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
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view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (2+) 11 5 6 18.8 8.7 5.8 21.8 10 8.3 15 15 15
y8 (2+) 13.9 6.2 7.7 11.8 11 8.5 18.2 12.6 11.5 15 15 15
y9 (2+) 8.8 4.3 3 15.2 9 3.4 17.6 10 4.5 15 15 15
y4 (1+) 12.1 6.5 10.8 15.8 7 12.2 19.9 9.6 16.3 15 15 15
y6 (1+) 13.3 4.5 3.1 13.9 11 4.9 19.2 11.9 5.8 15 15 15
sum 5.5 3.2 2.6 13.9 8.4 3 14.9 9 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments