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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Npr1 Npra
Sequence Length (AA) Molecular Weight (Da)
1057 119109
Protein Name
Atrial natriuretic peptide receptor 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPGSRRVRPR LRALLLLPPL LLLRSGHASD LTVAVVLPLT NTSYPWSWAR
60 70 80 90 100
VGPAVELALG RVKARPDLLP GWTVRMVLGS SENAAGVCSD TAAPLAAVDL
110 120 130 140 150
KWEHSPAVFL GPGCVYSAAP VGRFTAHWRV PLLTAGAPAL GIGVKDEYAL
160 170 180 190 200
TTRTGPSHVK LGDFVTALHR RLGWEHQALV LYADRLGDDR PCFFIVEGLY
210 220 230 240 250
MRVRERLNIT VNHQEFVEGD PDHYTKLLRT VQRKGRVIYI CSSPDAFRNL
260 270 280 290 300
MLLALDAGLT GEDYVFFHLD VFGQSLQGAQ GPVPRKPWER DDGQDRRARQ
310 320 330 340 350
AFQAAKIITY KEPDNPEYLE FLKQLKLLAD KKFNFTMEDG LKNIIPASFH
360 370 380 390 400
DGLLLYVQAV TETLAQGGTV TDGENITQRM WNRSFQGVTG YLKIDRNGDR
410 420 430 440 450
DTDFSLWDMD PETGAFRVVL NFNGTSQELM AVSEHRLYWP LGYPPPDIPK
460 470 480 490 500
CGFDNEDPAC NQDHFSTLEV LALVGSLSLV SFLIVSFFIY RKMQLEKELV
510 520 530 540 550
SELWRVRWED LQPSSLERHL RSAGSRLTLS GRGSNYGSLL TTEGQFQVFA
560 570 580 590 600
KTAYYKGNLV AVKRVNRKRI ELTRKVLFEL KHMRDVQNEH LTRFVGACTD
610 620 630 640 650
PPNICILTEY CPRGSLQDIL ENESITLDWM FRYSLTNDIV KGMLFLHNGA
660 670 680 690 700
IGSHGNLKSS NCVVDGRFVL KITDYGLESF RDPEPEQGHT LFAKKLWTAP
710 720 730 740 750
ELLRMASPPA RGSQAGDVYS FGIILQEIAL RSGVFYVEGL DLSPKEIIER
760 770 780 790 800
VTRGEQPPFR PSMDLQSHLE ELGQLMQRCW AEDPQERPPF QQIRLALRKF
810 820 830 840 850
NKENSSNILD NLLSRMEQYA NNLEELVEER TQAYLEEKRK AEALLYQILP
860 870 880 890 900
HSVAEQLKRG ETVQAEAFDS VTIYFSDIVG FTALSAESTP MQVVTLLNDL
910 920 930 940 950
YTCFDAVIDN FDVYKVETIG DAYMVVSGLP VRNGQLHARE VARMALALLD
960 970 980 990 1000
AVRSFRIRHR PQEQLRLRIG IHTGPVCAGV VGLKMPRYCL FGDTVNTASR
1010 1020 1030 1040 1050
MESNGEALRI HLSSETKAVL EEFDGFELEL RGDVEMKGKG KVRTYWLLGE
1057
RGCSTRG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3386 Collapse assay details

Data source: Panorama

Official Gene Symbol
Npr1
Peptide Sequence
ITDYGLESFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
672
Peptide End
681
CPTAC ID
non-CPTAC-3386
Peptide Molecular Mass
1,199.5823
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.4 5.4 8 17.6 9.7 8.6 18.4 11.1 11.7 15 15 15
y6 (1+) 3.6 8.4 5.8 16.6 9.9 6.3 17 13 8.6 15 15 15
y7 (1+) 5.5 4 3.8 17.2 10.2 4.5 18.1 11 5.9 15 15 15
y8 (1+) 5.6 6.8 7 12.2 8.5 10.8 13.4 10.9 12.9 15 15 15
y9 (1+) 8.2 5.5 6.1 16.1 8.7 7.7 18.1 10.3 9.8 15 15 15
sum 2.8 3.5 3.6 14.1 7.9 5.2 14.4 8.6 6.3 15 15 15


Additional Resources and Comments