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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

F13b

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
F13b Cf13b
Sequence Length (AA) Molecular Weight (Da)
669 76195
Protein Name
Coagulation factor XIII B chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMTLRHLPFI LLLILSGELY AEEKQCDFPT VENGRIAQYY YTFKSFYFPM
60 70 80 90 100
SVDKKLSFFC LAGYATESGK QEEQIRCTAE GWSPNPRCYK KCLKPDLRNG
110 120 130 140 150
YVSNDKVLYK LQERMSYGCS SGYKTTGGKD EEVVHCLSAG WSSQPSCRKE
160 170 180 190 200
QETCLAPELE HGNYSTTQRT FKVKDIVAYT CTAGYYTTTG KQTGEAECQA
210 220 230 240 250
NGWSLTPQCN KLMCSSLRLI ENGYFHPVKQ TYEEGDVVQF FCHENYYLSG
260 270 280 290 300
SDLIQCYNFG WYPESPICEG RRNRCPPPPV PLNSKIQPHS TTYRHGERVH
310 320 330 340 350
IECELNFVIQ GSEELLCENG KWTEPPKCIE EKEKVACEQP PSVENGVAHP
360 370 380 390 400
HSEIYYSGDK VTYRCGGGYS LRGSSTITCN RGRWTLPPEC VENIENCKPP
410 420 430 440 450
PDIANGVVVD GLLASYTTGS SVEYRCNEYY LLKGSETSRC EQGAWSSPPV
460 470 480 490 500
CLEPCTIDVD HMNRNNIQLK WKYEGKILHG DLIDFVCKQG YNLSPSIPLS
510 520 530 540 550
EISAQCNRGD VRYPMCIRKE SKGMCASPPV IRNGDIVSSA ARTYENGSSV
560 570 580 590 600
EYRCFDNHFL QGSQNVYCVD GVWTTPPSCL EPCTLSFVEM DKNYLQLKWN
610 620 630 640 650
FDNRPLILHG EYIEFMCKRD AYISETSIAG SVLRVQCDRG RLKYPKCTPR
660 669
DRRLSFQEAL RTRRQMEKR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3537 Collapse assay details

Data source: Panorama

Official Gene Symbol
F13b
Peptide Sequence
IAQYYYTFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
36
Peptide End
44
CPTAC ID
non-CPTAC-3537
Peptide Molecular Mass
1,195.5914
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 9.2 5.7 7.1 14.3 6.1 7.5 17 8.3 10.3 15 15 15
y7 (2+) 9.8 10.6 4.1 19.6 10.9 4.6 21.9 15.2 6.2 15 15 15
y6 (1+) 8.8 7.4 9.9 15.7 8.6 13 18 11.3 16.3 15 15 15
y7 (1+) 9.6 7.3 9.7 15.7 11.8 12.9 18.4 13.9 16.1 15 15 15
y8 (1+) 7.5 9.8 8.6 17.8 9.4 9.3 19.3 13.6 12.7 15 15 15
sum 3.7 2.6 3.1 15.3 5.2 5.9 15.7 5.8 6.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3398 Collapse assay details

Data source: Panorama

Official Gene Symbol
F13b
Peptide Sequence
LIENGYFHPVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
219
Peptide End
229
CPTAC ID
non-CPTAC-3398
Peptide Molecular Mass
1,315.6925
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 8.6 8.9 10 9.9 15.2 14.7 13.1 17.6 17.8 15 15 15
y8 (2+) 9.8 5.1 8 16.6 11.8 9 19.3 12.9 12 15 15 15
y9 (2+) 4.4 4 5.2 16.4 9.6 8.2 17 10.4 9.7 15 15 15
y10 (2+) 9 4.3 5.5 12.1 6.8 8.9 15.1 8 10.5 15 15 15
y3 (1+) 6.7 4.6 7.3 15.4 9.4 8.5 16.8 10.5 11.2 15 15 15
sum 3.2 3.2 3.9 14.8 8.6 7.1 15.1 9.2 8.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments