Angel1, CPTAC-3400 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Angel1	Kiaa0759

Sequence Length (AA)	Molecular Weight (Da)
667	75254

Protein Name
Protein angel homolog 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MIASCLYYLL	LPAARLFRFL	SDAFFTCRKN	ALLAKSSSPQ	VEGNFAMAPR
60	70	80	90	100
GPDQEECEGL	LQQWREEGWN	QTPSTASEGP	LADKGLAESS	LALLMDNSGE
110	120	130	140	150
QDAASEDKWS	SRQLSDLRAA	ENLNQPFPEV	LGEEPLAEVE	GPLWAAVPVQ
160	170	180	190	200
TGPQYADCAV	LPMGAMAAEQ	WEEDPAMVAW	SIAPEPMPQE	ETSMWPFEGL
210	220	230	240	250
EQLQPPPMEI	PYHEILWREW	EDFSTQPDAQ	GLEAGDGPQF	QFTLMSYNIL
260	270	280	290	300
AQDLMQQSSE	LYLHCHPDIL	NWNYRFANLM	QEFQHWDPDI	LCLQEVQEDH
310	320	330	340	350
YWEQLEPSLR	MMGFTCFYKR	RTGCKTDGCA	VCYKPTRFRL	LCASPVEYFR
360	370	380	390	400
PGLELLNRDN	VGLVLLLQPL	VPEGLGQVSV	APLCVANTHV	LYNPRRGDVK
410	420	430	440	450
LAQMAILLAE	VDKVARLSDG	SHCPIILCGD	LNSVPDSPLY	NFIRDGELQY
460	470	480	490	500
NGMPAWKVSG	QEDFSHQLYQ	RKLQAPLWPS	SLGITDCCQY	VTSCHPKRSE
510	520	530	540	550
RLKYGRDFLL	RFRFCDLACQ	RPVGLVLMEG	VTDTKPDRPA	GWAECIFEEE
560	570	580	590	600
ISELEPVFPR	TIGTIQHCLH	LTSVYTHFLP	QHGCPEVTTM	PLGLGMTVDY
610	620	630	640	650
IFFSAESCEN	ENRTDHRLDR	DGTLKLLGRL	SLLSEEILWA	ANGLPNPFYS
660	667
SDHLCLLASF	GMEVTAP

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3400 Collapse assay details

Data source: Panorama

Official Gene Symbol: Angel1
Peptide Sequence: NALLAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 30
Peptide End: 35

CPTAC ID: non-CPTAC-3400
Peptide Molecular Mass: 628.3908
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	23.8	7.2	9.8	25.9	9.5	9.2	35.2	11.9	13.4	15	15	15
y5 (2+)	40.7	24.8	16.1	37.3	21.6	16.5	55.2	32.9	23.1	15	15	15
y3 (1+)	10.3	4.8	8.8	13.3	8.4	9.8	16.8	9.7	13.2	15	15	15
y4 (1+)	7.1	5.4	3.7	11.9	12	8.7	13.9	13.2	9.5	15	15	15
y5 (1+)	7.6	3.9	8.6	11.9	6.9	9	14.1	7.9	12.4	15	15	15
sum	3.9	2	2.8	10.1	8.1	4.7	10.8	8.3	5.5	15	15	15

Angel1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3400 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Angel1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3400 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments