Art3, CPTAC-3404 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Art3	N/A

Sequence Length (AA)	Molecular Weight (Da)
371	42015

Protein Name
Ecto-ADP-ribosyltransferase 3

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKMGHFEMVT	TLLAAAVLMD	IFQVKAEVLD	MAENAFDDEY	LKCKSRMESK
60	70	80	90	100
YIPQMKREEW	ANDALLRMVW	DNAEIQWEAR	KAQLFLPRNF	KDTYGIALTA
110	120	130	140	150
YVNEAQEQTS	FYHTFSSAVK	MAGLSRRRYI	YNFPFKAFHF	YLVRALQLLR
160	170	180	190	200
RPCEKSYKTV	VYSTSPDISF	TFGEQNQARL	GNFTLAYSAK	PETADNQRVL
210	220	230	240	250
TIQTCFGVAV	GKFLNKEDDS	VVLIPLSEVF	QVSRKGTSND	LVLQSINSTC
260	270	280	290	300
SYYECAFLGG	LKTENCIANA	EYIDPRYLYN	PDMDNQKLED	SGRNNLDPDR
310	320	330	340	350
MPEIKVLQTE	ENPLLPDEKP	DRSRGKANNP	TPGLVPGPKS	HPSASSGNTL
360	370	371
LPSVMASTIL	LVASAVNFIE	L

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3483 Collapse assay details

Data source: Panorama

Official Gene Symbol: Art3
Peptide Sequence: AQLFLPR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 82
Peptide End: 88

CPTAC ID: non-CPTAC-3483
Peptide Molecular Mass: 843.4967
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	4.4	9.4	9.9	9.5	26.4	33.9	10.5	28	35.3	15	15	15
y4 (1+)	1.6	3.3	4.7	6.6	12.2	15.8	6.8	12.6	16.5	15	15	15
y2 (1+)	4.2	3.7	3.8	7.2	6.3	4.4	8.3	7.3	5.8	15	15	15
b2 (1+)	3	2.7	3	7	4.4	4.2	7.6	5.2	5.2	15	15	15
b3 (1+)	5.7	2	2.2	10	6.3	5.5	11.5	6.6	5.9	15	15	15
sum	1.7	5.1	5.7	7.4	13.9	18.8	7.6	14.8	19.6	15	15	15

Additional Resources and Comments

Resources

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View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-3404 Collapse assay details

Data source: Panorama

Official Gene Symbol: Art3
Peptide Sequence: NNLDPDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 294
Peptide End: 300

CPTAC ID: non-CPTAC-3404
Peptide Molecular Mass: 842.3882
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers