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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hp N/A
Sequence Length (AA) Molecular Weight (Da)
347 38752
Protein Name
Haptoglobin
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRALGAVVTL LLWGQLFAVE LGNDAMDFED DSCPKPPEIA NGYVEHLVRY
60 70 80 90 100
RCRQFYRLRA EGDGVYTLND EKQWVNTVAG EKLPECEAVC GKPKHPVDQV
110 120 130 140 150
QRIIGGSMDA KGSFPWQAKM ISRHGLTTGA TLISDQWLLT TAKNLFLNHS
160 170 180 190 200
ETASAKDITP TLTLYVGKNQ LVEIEKVVLH PNHSVVDIGL IKLKQRVLVT
210 220 230 240 250
ERVMPICLPS KDYIAPGRVG YVSGWGRNAN FRFTDRLKYV MLPVADQDKC
260 270 280 290 300
VVHYENSTVP EKKNLTSPVG VQPILNEHTF CAGLTKYQED TCYGDAGSAF
310 320 330 340 347
AIHDMEEDTW YAAGILSFDK SCAVAEYGVY VRATDLKDWV QETMAKN

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-671 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP
Peptide Modified Sequence
VVLHPN[+1.0]YSQVDIGLIK
Modification Type
Deamidated (NQ)
Protein - Site of Modification
241
Peptide - Site of Modification
6
Peptide Start
236
Peptide End
251
CPTAC ID
CPTAC-671
Peptide Molecular Mass
1,794.9880
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 7.2 12.9 8.8 11 12.6 9.6 13.1 18 13 15 15 15
y6 (1+) 7 10.6 7.1 7.4 10.1 10.9 10.2 14.6 13 15 15 15
b4 (1+) 6.4 13.9 9.3 7.6 13.8 11.4 9.9 19.6 14.7 15 15 15
sum 6.6 11.7 7.9 7.9 11.6 10.4 10.3 16.5 13.1 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-5609 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hp
Peptide Sequence
AEGDGVYTLNDEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
60
Peptide End
72
CPTAC ID
non-CPTAC-5609
Peptide Molecular Mass
1,409.6311
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 15.5 6.7 4.4 16.7 10 5.2 22.8 12 6.8 15 15 15
b4 (1+) 19.8 7.7 4.9 29 10 7 35.1 12.6 8.5 15 15 15
b2 (1+) 17.2 7.1 5.8 15.6 8.8 6.8 23.2 11.3 8.9 15 15 15
y6 (1+) 21.8 7.7 6.9 22.4 8.5 7.5 31.3 11.5 10.2 15 15 15
y7 (1+) 14.1 6.1 3.7 14.4 8.7 4.4 20.2 10.6 5.7 15 15 15
sum 6.7 3.1 2.2 7.3 6.8 3.9 9.9 7.5 4.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2671 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP
Peptide Sequence
DIAPTLTLYVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
216
Peptide End
227
CPTAC ID
non-CPTAC-2671
Peptide Molecular Mass
1,289.7231
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 6.9 5.6 3.3 12 10.6 5.6 13.8 12 6.5 15 15 15
b3 (1+) 7 4 3.8 13.4 9.1 6.1 15.1 9.9 7.2 15 15 15
y10 (2+) 6.1 6.2 3.9 13.3 10.5 5.4 14.6 12.2 6.7 15 15 15
y6 (1+) 7.9 6.2 3.8 12.4 12.1 5.3 14.7 13.6 6.5 15 15 15
y3 (1+) 5.1 6.1 4.6 11.1 11.4 6.6 12.2 12.9 8 15 15 15
sum 6.2 5 3 12.4 9.9 5.2 13.9 11.1 6 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3406 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hp
Peptide Sequence
NQLVEIEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
169
Peptide End
176
CPTAC ID
non-CPTAC-3406
Peptide Molecular Mass
971.5288
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 6.7 4.5 7.4 13 5.7 11.9 14.6 7.3 14 15 15 15
b2 (1+) 7.3 3.6 7 14.5 6.8 8.3 16.2 7.7 10.9 15 15 15
y4 (1+) 7.9 6.5 7.3 15.3 8.1 7.1 17.2 10.4 10.2 15 15 15
y5 (1+) 5.5 4.8 6 11.1 7.9 8.2 12.4 9.2 10.2 15 15 15
y6 (1+) 6.9 7 5.6 15.9 9.9 5.8 17.3 12.1 8.1 15 15 15
sum 4.1 3.6 3.9 13.2 6.1 4.8 13.8 7.1 6.2 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1128 Collapse assay details

Data source: Panorama

Official Gene Symbol
HP
Peptide Sequence
VGYVSGWGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
219
Peptide End
227
CPTAC ID
non-CPTAC-1128
Peptide Molecular Mass
979.4876
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 4.9 2.9 3.9 7.4 5.9 4.3 8.9 6.6 5.8 15 15 15
y4 (1+) 5.3 3.4 3.8 11.9 8 6 13 8.7 7.1 15 15 15
y5 (1+) 3.6 5.6 4 10.7 6.6 4.8 11.3 8.7 6.2 15 15 15
y6 (1+) 3.1 5.3 4.3 9.8 7.1 4.3 10.3 8.9 6.1 15 15 15
y8 (1+) 7.6 4.9 4.1 10.9 6.1 3.9 13.3 7.8 5.7 15 15 15
sum 2.7 3 2 9.3 5.2 2.6 9.7 6 3.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3407 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hp
Peptide Sequence
VGYVSGWGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
219
Peptide End
227
CPTAC ID
non-CPTAC-3407
Peptide Molecular Mass
979.4876
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 3.6 3.7 4.4 17 6.2 6.8 17.4 7.2 8.1 15 14 15
y4 (1+) 5.4 2.6 4.6 15.9 7.6 6.6 16.8 8 8 15 14 15
y5 (1+) 6.4 4.6 4.6 13.8 5 7.3 15.2 6.8 8.6 15 14 15
y6 (1+) 2.3 4.8 2.6 14.6 5.4 7.3 14.8 7.2 7.7 15 14 15
y8 (1+) 5 4.2 5.2 12.7 6 7.7 13.6 7.3 9.3 15 14 15
sum 2.8 1.8 1.9 14.3 4.6 5.8 14.6 4.9 6.1 15 14 15

Additional Resources and Comments