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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Col12a1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Col12a1 N/A
Sequence Length (AA) Molecular Weight (Da)
3120 340214
Protein Name
Collagen alpha-1(XII) chain
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQTRLPRALA ALGVALLLSS IEAEVDPPSD LNFKIIDENT VHMSWERPVD
60 70 80 90 100
PIVGYRITVD PTTDGPTKEF TLAASTTETL LSDLIPETQY VVTITSYNEV
110 120 130 140 150
EESVPVIGQL TIQTGGPTKP GEKKPGKTEI QKCSVSAWTD LVFLVDGSWS
160 170 180 190 200
VGRNNFKYIL DFIVALVSAF DIGEEKTRVG VVQYSSDTRT EFNLNQYYRR
210 220 230 240 250
EDLLAAVKKI PYKGGNTMTG DAIDYLVKNT FTESAGSRAG FPKVAIIITD
260 270 280 290 300
GKSQDEVEIP ARELRNIGVE VFSLGIKAAD AKELKQIAST PSLNHVFNVA
310 320 330 340 350
NFDAIVDIQN EIISQVCSGV DEQLGELVSG EEVIEPPSNL VVTELSSKYI
360 370 380 390 400
RLSWDPSPSA VTGYKILLTP MAAGSRHHAL SVGPQTTTLN VRDLTADTEY
410 420 430 440 450
QISVFAMKGL TSSEPTSVME KTQPMKVQVE CSRGVDIKAD IVFLVDGSYS
460 470 480 490 500
IGIANFVKVR AFLEVLAKSF EISPNRVQIS LVQYSRDPHT EFTLKEFNRV
510 520 530 540 550
EDIIKAINTF PYRGGSTNTG KAMTYVREKI FVPNKGSRSN VPKVMILITD
560 570 580 590 600
GKSSDAFRDP AIKLRNSDVE IFAVGVKDAV RSELEAIASP PAETHVFTVE
610 620 630 640 650
DFDAFQRISF ELTQSICLRI EQELAAIKKK AYVPPKDLRF TQVTANSFKA
660 670 680 690 700
EWSPPGDNVF SYHVTYKDAN GDDEVTVVEP ASSTSVVLNN LRPETLYLVN
710 720 730 740 750
VTAEYEDGFS VPITGEETTA EVKGVPRNLK VTDETTDSFK LTWSQAPGRV
760 770 780 790 800
LRYRIRYRPV SGGESKEVST PANQRRKTLE NLTPDTKYEI SVIAEYSSGP
810 820 830 840 850
GSPLTGNAAT EEVRGNPRDL RVSDATTSTL KLSWSRAPGK VKQYLVTYTP
860 870 880 890 900
AAGGETQEVT VRGDTTTTML RKLKEGTQYD LSVTALYASG AGEALSGKGS
910 920 930 940 950
TLEERGSPQN LVTKDITDTS IGAYWTSAPG MVRGYRVSWK SLYDDIEAGE
960 970 980 990 1000
TTLPGDAIHT MIENLQPETK YKISVFATYS SGEGEPVTGD ATTELSQDSK
1010 1020 1030 1040 1050
ILRVDEETEH TMRVTWKAAP GKVVNYRVVY RPQGGGRQMV AKVPPTVTST
1060 1070 1080 1090 1100
VLKRLQPQTT YDITVLPMYK TGEGKLRQGS GTTASRFKSP RNLKTSDPTM
1110 1120 1130 1140 1150
SSFRVTWEPA PGEVKGYKVT FHPTGDDRRL GELVLGPYDN TVVLEELRAG
1160 1170 1180 1190 1200
TTYRVNVFGM FDGGESLPLV GQEMTTLSDT TVTPFLSSGM DCLTRAEADI
1210 1220 1230 1240 1250
VLLVDGSWSI GRANFRTVRS FISRIVEVFE IGPKRVQIAL AQYSGDPRTE
1260 1270 1280 1290 1300
WQLNAHRDKK SLLQAVANLP YKGGNTLTGM ALNFIRQQSF KTQAGMRPRA
1310 1320 1330 1340 1350
RKIGVLITDG KSQDDVEAPS KKLKDEGVEL FAIGIKNADE VELKMIATDP
1360 1370 1380 1390 1400
DDTHAYNVAD FESLSKIVDD LTINLCNSVK GPGDLEAPTN LVISERTHRS
1410 1420 1430 1440 1450
FRVSWTPPSD SVDRYKVEYY PVSGGKRQEF YVSRLDTSTV LKDLKPETDY
1460 1470 1480 1490 1500
VVNVYSVVED EYSEPLKGTE KTLPVPVVSL NIYDVGPTTM HVQWQPVGGA
1510 1520 1530 1540 1550
TGYTVSYQPT RSPEGTKPKE MRVGPTVNDV QLTGLLPNTE YEVTVQAVLY
1560 1570 1580 1590 1600
DLTSEPAKAR EVTLPLPRPQ DVKLRDVTHS TMNVVWEPVL GKVRKYIVRY
1610 1620 1630 1640 1650
KTPDEEFKEV EVDRSRASTI LKDLSSQTQY TVSVSAVYDE GTSPPATAYD
1660 1670 1680 1690 1700
TTRRVPAPTN LQFTEVTPES FRGTWDHGAS DVSLYRITWA PVGNPDKMET
1710 1720 1730 1740 1750
ILNGDENTLV FENLNPNTPY EVSITAIYPD ESESEDLSGT ERTLRLIPLT
1760 1770 1780 1790 1800
TQAPKSGPRN LQVYNATSNS LTVKWDPASG RVQKYRITYQ PSTGEGNEQT
1810 1820 1830 1840 1850
ITVGGRQNSV LLQKLKPDTP YTITVYSQYP DGEGGRMTGR GKTKPLNTVR
1860 1870 1880 1890 1900
NLRVYDPSTS SLSVRWDHAE GNPRQYKLFY APTSGGPEEL VPIPGNTNYA
1910 1920 1930 1940 1950
ILRNLQPDTP YTITVVPVYT EGDGGRTSDT GRTLVRGLAR NIQVYNPTPN
1960 1970 1980 1990 2000
SLDVRWDPAP GPVQQYRIVY SPVAGTRPSE SIVVPGNTRT VHLERLIPDT
2010 2020 2030 2040 2050
PYSVNIVALY SDGEGNPSPS QGRTLPRSGP RNIRVFGETT NSLSVAWDHA
2060 2070 2080 2090 2100
DGPVQQYRII YSPTVGDPID EYTTVPGRRN NVILQPLQPD TPYKITVIAI
2110 2120 2130 2140 2150
YEDGDGGHLT GNGRTVGLLP PQNIHIFDEW YTRFRVSWDP SPSPVLGYKI
2160 2170 2180 2190 2200
VYKPVGSNEP MEAFVGEVTS YTLHNLNPST TYDVSVYAQY DSGLSVPLTD
2210 2220 2230 2240 2250
QGTTLYLNVT DLKTYQVGWD TFCVKWSPHR AATSYRLKLS PADGTRGQEI
2260 2270 2280 2290 2300
TVRGSETSHC FTGLSPEAEY GVTVFVQTPN LEGPGVPIKE QTTVKPTEAP
2310 2320 2330 2340 2350
TEPPTPSPPP TIPPARDVCK GAKADIVFLT DASWSIGDDN FNKVVKFIFN
2360 2370 2380 2390 2400
TVGAFDEVNP AGIQVSFVQY SDEVKSEFKL NTYNDKALAL GALQNIRYRG
2410 2420 2430 2440 2450
GNTRTGKALT FIKEKVLTWE SGMRKNVPKV LVVVTDGRSQ DEVKKAAFVI
2460 2470 2480 2490 2500
QQSGFSVFVV GVADVDYNEL ANIASKPSER HVFIVDDFES FEKIEDNLIT
2510 2520 2530 2540 2550
FVCETATSSC PLIYLDGYTS PGFKMLEAYN LTEKNFASVQ GVSLESGSFP
2560 2570 2580 2590 2600
SYSAYRLQKN AFINQPTAEL HPNGLPPSYT IILLFRLLPE TPSDPFAIWQ
2610 2620 2630 2640 2650
ITDRDYRPQV GVIADPSSKT LSFFNKDTRG EVQTVTFDTD EVKTLFYGSF
2660 2670 2680 2690 2700
HKVHIVVTSK SVKIYIDCYE IIEKDIKEAG NITTDGYEIL GKLLKGERKS
2710 2720 2730 2740 2750
ATFQIQSFDI VCSPVWTSRD RCCDIPSRRD EAKCPALPNA CTCTQDSVGP
2760 2770 2780 2790 2800
PGPPGPAGGP GAKGPRGERG INGAVGPPGP RGDTGPPGPQ GPPGPQGPNG
2810 2820 2830 2840 2850
LSIPGEQGRQ GMKGDAGEPG LPGRTGTPGL PGPPGPMGPP GDRGFTGKDG
2860 2870 2880 2890 2900
AMGPRGPPGP PGSPGSPGVT GPSGKPGKPG DHGRPGQSGL KGEKGDRGDI
2910 2920 2930 2940 2950
ASQNMMRAVA RQVCEQLISG QMSRFNQMLN QIPNDYHSSR NQPGPPGPPG
2960 2970 2980 2990 3000
PPGSAGARGE PGPGGRPGFP GTPGMQGPPG ERGLPGEKGE RGTGSQGPRG
3010 3020 3030 3040 3050
PPGPPGPQGE SRTGPPGSTG SRGPPGPPGR PGNSGIRGPP GPPGYCDSSQ
3060 3070 3080 3090 3100
CASIPYNGQG YPEPYVPEGG AYLPEREPFI VPVEPERTAE YEDDYGADEP
3110 3120
DQQHPDHMRW RRALRPGPAE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3417 Collapse assay details

Data source: Panorama

Official Gene Symbol
Col12a1
Peptide Sequence
TPDEEFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1602
Peptide End
1608
CPTAC ID
non-CPTAC-3417
Peptide Molecular Mass
864.3865
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (2+) 6.6 4.8 5.7 15 8.1 7.4 16.4 9.4 9.3 15 15 15
y2 (1+) 4.2 3.6 7.8 16.8 11.9 8 17.3 12.4 11.2 15 15 15
y3 (1+) 54.9 23.6 13.8 85.8 78.5 73.8 101.9 82 75.1 15 15 15
y5 (1+) 5.4 6.5 3.7 17.1 6.5 3.8 17.9 9.2 5.3 15 15 15
y6 (1+) 6.7 4.9 5.6 15.6 6.6 5.8 17 8.2 8.1 15 15 15
sum 5.4 3.5 3.8 16.1 7.7 4.5 17 8.5 5.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments