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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ltbp4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ltbp4 N/A
Sequence Length (AA) Molecular Weight (Da)
1666 178642
Protein Name
Latent-transforming growth factor beta-binding protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRRPGLGGPC PLLLLLLLPA ATSASGSSPS PSPSPIEKAV VPSHQAGVAA
60 70 80 90 100
CHCCLDQTPK SSRCTRASCR VRNCPPAKCT GLEGCLTPTP SVPSPSRSPV
110 120 130 140 150
EKSQVSLNWQ PLTLQEARAL LRQRRPRGPW ARALLKRRPP HRAPAGQARV
160 170 180 190 200
LCPLICHNGG VCVKPDRCLC PPDFAGKFCQ LHSSGARPPA PAMPGLTRSV
210 220 230 240 250
YTMPLANHRD DEHGVASMVS VHVEHPQEAS VVVHQVERVS GPWEEANPEA
260 270 280 290 300
LARAEAAARA EAAAPYTVLA QSAPREDGYS DASGFGYCFR ELRGSECASP
310 320 330 340 350
LPGLRTQEVC CRGEGLAWGV HDCHPCAEHL RNSNQVSGPN GPCPPGFERV
360 370 380 390 400
NGSCVDVDEC ATGGRCQHGE CANTRGGYTC VCPDGFLLDS SRSSCISQHV
410 420 430 440 450
ISEAKGPCYR VLHDGGCSLP ILRNITKQIC CCSRVGKAWG RGCQLCPPYG
460 470 480 490 500
SEGFREICPA GPGYHYSASD LRYNTRPLNQ DPPRVTFNQP RVPPATPRPP
510 520 530 540 550
TGFLPTRRPE PRPDPGPQPE PRPRPEPRPR PESRPRPEPR PRPEPRPQPE
560 570 580 590 600
SQPRPESRPR PESQPWPEFP LPSIPAWTGP EIPESGPSSS MCQRNPQVCG
610 620 630 640 650
PGRCVPRPSG YTCACDPGFR LGPQGTRCID IDECRRVPTP CAPGRCENTP
660 670 680 690 700
GSFRCVCGTG FQAGPRATEC LDVDECRRVP PPCDRGRCEN TPGSFLCVCP
710 720 730 740 750
AGYQAAPHGA SCQDVDECTQ SPGLCGRGVC ENLPGSFRCV CPAGFRGSAC
760 770 780 790 800
EEDVDECAQQ PPPCGPGRCD NTAGSFHCAC PAGFRSRGPG APCQDVDECS
810 820 830 840 850
RSPSPCAYGR CENTEGSFKC VCPTGFQPNA AGSECEDVDE CENRLACPGQ
860 870 880 890 900
ECVNSPGSFQ CRACPVGHHL HRGRCTDVDE CSSGTPCGLH GQCTNTKGSF
910 920 930 940 950
HCSCSTGYRA PSGQPGPCAD INECLEGDFC FPHGECLNTD GSFTCTCAPG
960 970 980 990 1000
YRPGPRGASC LDVDECSEED LCQSGICTNT DGSFECICPP GHRAGPDLAS
1010 1020 1030 1040 1050
CLDIDECRER GPALCGSQRC ENSPGSYRCV RDCDPGYHPG PEGTCDDIDE
1060 1070 1080 1090 1100
CREYGSAICG AQRCENTPGS YRCTPACDPG YQPTPGGGCQ DVDECRNRSF
1110 1120 1130 1140 1150
CGAHAMCQNL PGSFQCVCDQ GYEGARDGRH CVDVNECETL QGVCGSALCE
1160 1170 1180 1190 1200
NVEGSFLCVC PNSPEEFDPM TGRCVPPRAP AGTFPGSQPQ APASPSLPAR
1210 1220 1230 1240 1250
PPAPPPPRRP SPPRQGPVSS GRRECYFDTA APDACDNILA RNVTWQECCC
1260 1270 1280 1290 1300
TVGEGWGSGC RIQQCPGTET AEYQSLCPHG RGYLVPSGDL SARRDVDECQ
1310 1320 1330 1340 1350
LFQDQVCKSG VCVNTAPGYS CYCSNGFYYH AHRLECVDND ECADEEPACE
1360 1370 1380 1390 1400
GGRCVNTVGS YHCTCEPPLV LDGSRRRCVS NESQSLDDNL GVCWQEVGPD
1410 1420 1430 1440 1450
LVCSRPRLDR QATYTECCCL YGEAWGMDCA LCPAQDSDDF EALCNVLRPP
1460 1470 1480 1490 1500
AYGPPRPGGF GIPYEYGPDI GPPYQSLPYG PDLYPPPVLP YDPYPPPPGP
1510 1520 1530 1540 1550
FARREAPYGA PPFDMPDFED DGGPYGESET PDPPSRGTGW PYRSRDTRGS
1560 1570 1580 1590 1600
FPEPEESSER GSYTGALSEP YEGLEAEECG ILDGCPHGRC VRVPEGFTCD
1610 1620 1630 1640 1650
CFDGYRLDIT RMSCVDVNEC DEAEATSPLC VNARCVNTDG SFRCICRPGF
1660 1666
APTHQPHHCA PARPRA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3427 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ltbp4
Peptide Sequence
VTFNQPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
485
Peptide End
491
CPTAC ID
non-CPTAC-3427
Peptide Molecular Mass
860.4505
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 4.6 6.7 6.7 12.9 8.7 7.7 13.7 11 10.2 15 15 15
y5 (2+) 10.2 9.5 8.9 14.3 8.7 9.7 17.6 12.9 13.2 15 15 15
y4 (1+) 5.6 8.4 4.9 8.8 12.8 7.3 10.4 15.3 8.8 15 15 15
y5 (1+) 6.6 6.4 7.3 13.5 9.3 8.2 15 11.3 11 15 15 15
y6 (1+) 8.8 7.1 5.8 15.6 11.3 11.4 17.9 13.3 12.8 15 15 15
sum 3.9 3.5 3.6 11.6 8.6 5.3 12.2 9.3 6.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments