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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Masp2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Masp2 N/A
Sequence Length (AA) Molecular Weight (Da)
685 75517
Protein Name
Mannan-binding lectin serine protease 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRLLIFLGLL WSLVATLLGS KWPEPVFGRL VSPGFPEKYA DHQDRSWTLT
60 70 80 90 100
APPGYRLRLY FTHFDLELSY RCEYDFVKLS SGTKVLATLC GQESTDTEQA
110 120 130 140 150
PGNDTFYSLG PSLKVTFHSD YSNEKPFTGF EAFYAAEDVD ECRVSLGDSV
160 170 180 190 200
PCDHYCHNYL GGYYCSCRAG YVLHQNKHTC SALCSGQVFT GRSGYLSSPE
210 220 230 240 250
YPQPYPKLSS CTYSIRLEDG FSVILDFVES FDVETHPEAQ CPYDSLKIQT
260 270 280 290 300
DKGEHGPFCG KTLPPRIETD SHKVTITFAT DESGNHTGWK IHYTSTARPC
310 320 330 340 350
PDPTAPPNGS ISPVQAIYVL KDRFSVFCKT GFELLQGSVP LKSFTAVCQK
360 370 380 390 400
DGSWDRPMPE CSIIDCGPPD DLPNGHVDYI TGPEVTTYKA VIQYSCEETF
410 420 430 440 450
YTMSSNGKYV CEADGFWTSS KGEKLPPVCE PVCGLSTHTI GGRIVGGQPA
460 470 480 490 500
KPGDFPWQVL LLGQTTAAAG ALIHDNWVLT AAHAVYEKRM AASSLNIRMG
510 520 530 540 550
ILKRLSPHYT QAWPEEIFIH EGYTHGAGFD NDIALIKLKN KVTINGSIMP
560 570 580 590 600
VCLPRKEAAS LMRTDFTGTV AGWGLTQKGL LARNLMFVDI PIADHQKCTA
610 620 630 640 650
VYEKLYPGVR VSANMLCAGL ETGGKDSCRG DSGGALVFLD NETQRWFVGG
660 670 680 685
IVSWGSINCG AADQYGVYTK VINYIPWIEN IISNF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3722 Collapse assay details

Data source: Panorama

Official Gene Symbol
Masp2
Peptide Sequence
LVSPGFPEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
30
Peptide End
38
CPTAC ID
non-CPTAC-3722
Peptide Molecular Mass
972.5280
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 7.1 4.1 4 7.5 4.6 6.4 10.3 6.2 7.5 15 15 15
y7 (2+) 18.5 6.3 4.1 15.8 7.2 8.5 24.3 9.6 9.4 15 15 15
y3 (1+) 28.9 6.2 5.8 25.7 10.6 6.1 38.7 12.3 8.4 15 15 15
y6 (1+) 8.2 4.3 3.8 8.6 5 7.7 11.9 6.6 8.6 15 15 15
y7 (1+) 4.9 6.2 4.5 4.4 7.8 9 6.6 10 10.1 15 15 15
sum 2.9 3.5 2.2 2.4 4.9 7.1 3.8 6 7.4 15 15 15

Additional Resources and Comments

Resources
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Assay Details for non-CPTAC-2683 Collapse assay details

Data source: Panorama

Official Gene Symbol
MASP2
Peptide Sequence
WPEPVFGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
22
Peptide End
29
CPTAC ID
non-CPTAC-2683
Peptide Molecular Mass
986.4974
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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view larger image

Response Curves

Data source: Panorama

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view larger image

Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 6.3 4.7 9 11.9 8.5 10.4 13.5 9.7 13.8 15 15 15
y7 (1+) 8.4 5.8 9.9 11.3 7.1 9.8 14.1 9.2 13.9 15 15 15
y6 (1+) 9.8 6.6 9.1 17.1 9.4 10.8 19.7 11.5 14.1 15 15 15
y3 (1+) 30.3 7 8.6 40.8 12.8 11.2 50.8 14.6 14.1 15 15 15
y5 (2+) 22.3 8 6.9 28.3 11.1 7.3 36 13.7 10 15 15 15
sum 4.8 3.9 7.8 12.2 7.5 9.1 13.1 8.5 12 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3430 Collapse assay details

Data source: Panorama

Official Gene Symbol
Masp2
Peptide Sequence
WPEPVFGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
22
Peptide End
29
CPTAC ID
non-CPTAC-3430
Peptide Molecular Mass
986.4974
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 6 4.1 4.8 14 5.2 7.8 15.2 6.6 9.2 15 14 15
y6 (1+) 14.9 3.2 2.7 16.5 5.5 3.3 22.2 6.4 4.3 15 14 15
y5 (1+) 6.8 3 3 10.2 7.4 3.8 12.3 8 4.8 15 14 15
y3 (1+) 31.2 2.9 4.4 50.5 7.4 5.4 59.4 7.9 7 15 14 15
y5 (2+) 29.7 3.5 2.8 28.3 9.2 6.3 41 9.8 6.9 15 14 15
sum 5.2 1.8 1.5 10.2 5.9 3.1 11.4 6.2 3.4 15 14 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments