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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ppfia1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ppfia1 N/A
Sequence Length (AA) Molecular Weight (Da)
1266 142676
Protein Name
Ppfia1 protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMCEVMPTIS EAEGPPGGGG SHGSGSPSQP DADSHFEQLM VSMLEERDRL
60 70 80 90 100
LDTLRETQET LALTQGKLHE VGHERDSLQR QLNTALPQEF AALTKELNVC
110 120 130 140 150
REQLLEREEE IAELKAERNN TRLLLEHLEC LVSRHERSLR MTVVKRQAQS
160 170 180 190 200
PAGVSSEVEV LKALKSLFEH HKALDEKVRE RLRVALERCS LLEEELGATH
210 220 230 240 250
KELMILKEQN NQKKTLTDGL LDGNHEQESA PSTNGKRSSD GSLSHEDLAK
260 270 280 290 300
VLELQEVIDR QAREQSQMKE RLASLSSHAA ELEEDLDTAR KDLIKSEEMN
310 320 330 340 350
TKLQREVREA MAQKEDMEER ITTLEKRYLA AQREATSVHD LNDKLENEIA
360 370 380 390 400
NKDSMHRQTE DKNRQLQERL ELAEQKLQQT LRKAETLPEV EAELAQRVAA
410 420 430 440 450
LSKSGPLSSG SSAAKEAKLL ELTSKLRKAE ERHGNIEERL RQMEAQLEEK
460 470 480 490 500
NQELQRARQR EKMNEEHNKR LSDTVDKLLS ESNERLQLHL KERMAALEDK
510 520 530 540 550
NSLLREVENA KKQLEETQHD KDQLVVTIEA LKAELEQMRL RGPSLHHGRP
560 570 580 590 600
HLGSVPDFRF SVADGHVDAY STSAVLRRPQ KGRLAALRDE PSKVQTLNEQ
610 620 630 640 650
DWERAQQASV LANVAQAFES DVDVSDGEDD RDTLLSSVDL LSPSGQADAQ
660 670 680 690 700
TLAMMLQEQL DAINKEIRLI QEEKENTEQR AEEIESRVGS GSLDNLGRFR
710 720 730 740 750
SMSSIPPYPA SSLAGSSPPG SGRSTPRRVP HSPAREVDRL GVMTLPSDLR
760 770 780 790 800
KHRRQLPASR EEVRDDKTTI KCETSPPSSP RPLRLDRMHK GAPHTVSHED
810 820 830 840 850
IRDLRNSTGS QDGPVSNPSS SNSSQDSLHK APKKKGIKSS IGRLFGKKEK
860 870 880 890 900
GRPGPLGKES PGQVGVSETE NSSQDALGLS KLGGQAEKNR KLQKKHELLE
910 920 930 940 950
EARRQGLPFA QWDGPTVVVW LELWVGMPAW YVAACRANVK SGAIMSALSD
960 970 980 990 1000
TEIQREIGIS NPLHRLKLRL AIQEIMSLTS PSAPPTSRTT TGNVWLTHEE
1010 1020 1030 1040 1050
METLTATPQT EDEEGSWAQT LAYGDMNHEW IGNEWLPSLG LPQYRSYFME
1060 1070 1080 1090 1100
CLVDARMLDH LTKKDLRGQL KMVDSFHRNS FQCGIMCLRR LNYDRKELER
1110 1120 1130 1140 1150
KREESQNETR DVLVWSNDRV IRWILSIGLK EYANNLIESG VHGALLALDE
1160 1170 1180 1190 1200
TFDFSALALL LQIPTQNTQA RAVLEREFNN LLITGTDRRF DEDDDKSFRR
1210 1220 1230 1240 1250
APSWRKKFRP KDIRGLASGS AETLPANFRV TSSMSSPSMQ PKKVQMDGSV
1260 1266
SGAQRLDSAT VRTYSC

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3436 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ppfia1
Peptide Sequence
LELAEQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
369
Peptide End
376
CPTAC ID
non-CPTAC-3436
Peptide Molecular Mass
829.4545
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b4 (2+) 57.4 48 38.1 121.1 54.9 51.9 134 72.9 64.4 15 15 15
b3 (1+) 13.2 8.3 10.7 17.2 7.8 11 21.7 11.4 15.3 15 15 15
y4 (1+) 3.9 6.5 4.8 14 8.9 4.4 14.5 11 6.5 15 15 15
y5 (1+) 4.2 3.5 3.4 14.1 7.5 6.3 14.7 8.3 7.2 15 15 15
y6 (1+) 4.3 5.5 5.5 12.5 9.7 7.1 13.2 11.2 9 15 15 15
sum 3.5 2.9 2.9 13.6 7.1 5.2 14 7.7 6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments